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15N-labed glycine synthesis Anais da ABC (AABC)
Tavares,Claudinéia R.O.; Bendassolli,José A.; Coelho,Fernando; Sant'ana Filho,Carlos R.; Prestes,Clelber V..
This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable 15NH3(aq) volumes in the reaction were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stable isotope; Glycine; Amino acid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300005
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