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Aphotic N2 fixation along an oligotrophic to ultraoligotrophic transect in the Western Tropical South Pacific Ocean ArchiMer
Benavides, Mar; Shoemaker, Katyanne M.; Moisander, Pia H.; Niggemann, Jutta; Dittmar, Thorsten; Duhamel, Solange; Grosso, Olivier; Pujo-pay, Mireille; Hélias-nunige, Sandra; Bonnet, Sophie.
The western tropical South Pacific (WTSP) Ocean has been recognized as a global hotspot of dinitrogen (N2) fixation. Here, as in other marine environments across the oceans, N2 fixation studies have focused in the sunlit layer. However, studies have confirmed the importance of aphotic N2 fixation activity, although until now only one had been performed in the WTSP. In order to increase our knowledge of aphotic N2 fixation in the WTSP, here we measure N2 fixation rates and identify diazotrophic phylotypes in the mesopelagic layer along a transect spanning from New Caledonia to French Polynesia. Because non-cyanobacterial diazotrophs presumably need external dissolved organic matter (DOM) sources for their nutrition, we also identified DOM compounds using...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00420/53118/54070.pdf
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Diversity of total and active free-living vs. particle-attached bacteria in the euphotic zone of the NW Mediterranean Sea ArchiMer
Ghiglione, Jean Francois; Conan, Pascal; Pujo-pay, Mireille.
The structure of the total and metabolically active communities of attached and free-living bacteria were analysed in the euphotic zone in the NW Mediterranean Sea with the use of DNA- and RNA-derived capillary electrophoresis single-strand conformation polymorphism fingerprinting. More than half (between 52% and 69%) of the DNA- derived operational taxonomic units (OTUs) were common in both attached and free-living fractions in the euphotic layer, suggesting an exchange or co-occurrence between them. However, analysis targeting 16S rRNA showed that only some of them were found in the dominant active bacterial pool. Especially at the deep chlorophyll maximum, less than half of the attached bacterial populations were found to be active, with regard to the...
Tipo: Text Palavras-chave: Particle-attached bacteria; CE-SSCP; DNA/RNA.
Ano: 2009 URL: https://archimer.ifremer.fr/doc/00243/35432/33966.pdf
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Dynamics and controls of heterotrophic prokaryotic production in the western tropical South Pacific Ocean: links with diazotrophic and photosynthetic activity ArchiMer
Van Wambeke, France; Gimenez, Audrey; Duhamel, Solange; Dupouy, Cecile; Lefevre, Dominique; Pujo-pay, Mireille; Moutin, Thierry.
Heterotrophic prokaryotic production (BP) was studied in the western tropical South Pacific (WTSP) using the leucine technique, revealing spatial and temporal variability within the region. Integrated over the euphotic zone, BP ranged from 58 to 120 mg C m−2 d−1 within the Melanesian Archipelago, and from 31 to 50 mg C m−2 d−1 within the western subtropical gyre. The collapse of a bloom was followed during 6 days in the south of Vanuatu using a Lagrangian sampling strategy. During this period, rapid evolution was observed in the three main parameters influencing the metabolic state: BP, primary production (PP) and bacterial growth efficiency. With N2 fixation being one of the most important fluxes fueling new production, we explored relationships between...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00448/56007/57515.pdf
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Dynamics of particulate organic matter composition in coastal systems: a spatio-temporal study at multi-systems scale ArchiMer
Lienart, Camilla; Savoye, Nicolas; Bozec, Yann; Breton, Elsa; Conan, Pascal; David, Valerie; Feunteun, Eric; Grangere, Karine; Kerherve, Philippe; Lebreton, Benoit; Lefebvre, Sebastien; L'Helguen, Stephane; Mousseau, Laure; Raimbault, Patrick; Richard, Pierre; Riera, Pascal; Sauriau, Pierre-guy; Schaal, Gauthier; Aubert, Fabien; Aubin, Sebastien; Bichon, Sabrina; Boinet, Christophe; Bourasseau, Line; Breret, Martine; Caparros, Jocelyne; Cariou, Thierry; Charlier, Karine; Claquin, Pascal; Cornille, Vincent; Corre, Anne-marie; Costes, Laurence; Crispi, Olivier; Crouvoisier, Muriel; Czamanski, Marie; Del Amo, Yolanda; Derriennic, Herve; Dindinaud, Francois; Durozieri, Maia; Hanquiez, Vincent; Nowaczyk, Antoine; Devesa, Jeremy; Ferreira, Sophie; Fornier, Michel; Garcia, Fabrice; Garcia, Nicole; Geslin, Sandrine; Grossteffan, Emilie; Gueux, Aurore; Guillaudeau, Julien; Guillou, Gael; Joly, Orianne; Lachaussee, Nicolas; Lafont, Michel; Lamoureux, Jezabel; Lecuyer, Eric; Lehodey, Jean-paul; Lemeille, David; Leroux, Cedric; Mace, Eric; Maria, Eric; Pineau, Philippe; Petit, Franck; Pujo-pay, Mireille; Rimelin-maury, Peggy; Sultan, Emmanuelle.
In coastal systems, the multiplicity of sources fueling the pool of particulate organic matter (POM) leads to divergent estimations of POM composition. Eleven systems (two littoral systems, eight embayments and semi-enclosed systems and one estuary) distributed along the three maritime façades of France were studied for two to eight years in order to quantify the relative contribution of organic matter sources to the surface-water POM pool in coastal systems. This study was based on carbon and nitrogen elemental and isotopic ratios, used for running mixing models. The POM of the estuary is dominated by terrestrial material (93% on average), whereas the POM of the other systems is dominated by phytoplankton (84% on average). Nevertheless, for the latter...
Tipo: Text Palavras-chave: Particulate organic matter; Coastal systems; C and N stable isotopes; C:N ratio; Mixing model.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00374/48550/48871.pdf
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Dynamics of particulate organic matter composition in coastal systems: forcing of spatio-temporal variability at multi-systems scale ArchiMer
Lienart, Camilla; Savoye, Nicolas; David, Valerie; Ramond, Pierre; Tress, Paco Rodriguez; Hanquiez, Vincent; Marieu, Vincent; Aubert, Fabien; Aubin, Sebastien; Bichon, Sabrina; Boinet, Christophe; Bourasseau, Line; Bozec, Yann; Breret, Martine; Breton, Elsa; Caparros, Jocelyne; Cariou, Thierry; Claquin, Pascal; Conan, Pascal; Corre, Anne-marie; Costes, Laurence; Crouvoisier, Muriel; Del Amo, Yolanda; Derriennic, Herve; Dindinaud, Francois; Duran, Robert; Durozier, Maia; Devesa, Jeremy; Ferreira, Sophie; Feunteun, Eric; Garcia, Nicole; Geslin, Sandrine; Grossteffan, Emilie; Gueux, Aurore; Guillaudeau, Julien; Guillou, Gael; Jolly, Orianne; Lachaussee, Nicolas; Lafont, Michel; Lagadec, Veronique; Lamoureux, Jezabel; Lauga, Beatrice; Lebreton, Benoit; Lecuyer, Eric; Lehodey, Jean-paul; Leroux, Cedric; L'Helguen, Stephane; Mace, Eric; Maria, Eric; Mousseau, Laure; Nowaczyk, Antoine; Pineau, Philippe; Petit, Franck; Pujo-pay, Mireille; Raimbault, Patrick; Rimmelin-maury, Peggy; Rouaud, Vanessa; Sauriau, Pierre-guy; Sultan, Emmanuelle; Susperregui, Nicolas.
In costal systems, particulate organic matter (POM) results from a multiplicity of sources having their respective dynamics in terms of production, decomposition, transport and burial. The POM pool experiences thus considerable spatial and temporal variability. In order to better understand this variability, the present study employs statistical multivariate analyses to investigate links between POM composition and environmental forcings for a panel of twelve coastal systems distributed along the three maritime regions of France and monitored weekly to monthly for 1 to 8 years. At multi-system scale, two main gradients of POC composition have been identified: a ‘Continent-Ocean’ gradient associated with hydrodynamics, sedimentary dynamics and depth of the...
Tipo: Text Palavras-chave: Particulate organic matter; Coastal systems; Environmental forcing; Meta-analysis; France.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00433/54471/55848.pdf
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Expanding Tara Oceans Protocols for Underway, Ecosystemic Sampling of the Ocean-Atmosphere Interface During Tara Pacific Expedition (2016-2018) ArchiMer
Gorsky, Gabriel; Bourdin, Guillaume; Lombard, Fabien; Pedrotti, Maria Luiza; Audrain, Samuel; Bin, Nicolas; Boss, Emmanuel; Bowler, Chris; Cassar, Nicolas; Caudan, Loic; Chabot, Genevieve; Cohen, Natalie R.; Cron, Daniel; De Vargas, Colomban; Dolan, John R.; Douville, Eric; Elineau, Amanda; Flores, J. Michel; Ghiglione, Jean Francois; Haentjens, Nils; Hertau, Martin; John, Seth G.; Kelly, Rachel L.; Koren, Ilan; Lin, Yajuan; Marie, Dominique; Moulin, Clementine; Moucherie, Yohann; Pesant, Stephane; Picheral, Marc; Poulain, Julie; Pujo-pay, Mireille; Reverdin, Gilles; Romac, Sarah; Sullivan, Mathew B.; Trainic, Miri; Tressol, Marc; Trouble, Romain; Vardi, Assaf; Voolstra, Christian R.; Wincker, Patrick; Agostini, Sylvain; Banaigs, Bernard; Boissin, Emilie; Forcioli, Didier; Furla, Paola; Galand, Pierre E.; Gilson, Eric; Reynaud, Stephanie; Sunagawa, Shinichi; Thomas, Olivier P.; Thurber, Rebecca Lisette Vega; Zoccola, Didier; Planes, Serge; Allemand, Denis; Karsenti, Eric; Planes, S.; Banaig, B.; Boissin, E.; Iwankow, G.; Allemand, D.; Zoccola, D.; Reynaud, S.; Beraud, E.; Djerbi, N.; Forcioli, D.; Furla, P.; Gilson, E.; Mcmind, R.; Ottaviani, A.; Rottinger, E.; Rouan, A.; Zamoum, T.; Flume, B. C. C.; Pogoreutz, C.; Voolstra, C. R.; Rothig, T.; Ziegler, M.; Paoli, L.; Ruscheweyh, H-j; Salazar, G.; Sunagawa, S.; Flores, J. M.; Koren, I; Trainic, M.; Lang-yona, N.; Vardi, A.; Conan, P.; Ghiglione, J-f; Pujo-pay, M.; Galand, P. E.; Hochart, C.; Audrain, S.; Bourgois, E.; Hertau, M.; Lancelot, J.; Monmarche, D.; Moulin, C.; Moucherie, Y.; Trouble, R.; Boss, E.; Bourdin, G.; Haentjens, N.; Karp-boss, L.; Douville, Eric; Agostini, S.; Mitsuhashi, G.; Kitano, Y.; Da Silva, O.; Dolan, J. R.; Gorsky, G.; Lemee, R.; Lombard, F.; Pedrotti, M-l; Cronin, D.; Sullivan, M.; Armstrong, E.; Aury, J-m; Barbe, V; Belser, C.; Carradec, Q.; Labadie, K.; Le-hoang, J.; Noel, B.; Poulain, J.; Wincker, P.; Klinges, G.; Vega-thunder, R.; Bonnival, E.; De Vargas, C.; Henry, N.; Marie, D.; Romac, S.; Pesant, S.; Miguel-gorda, M.; Thomas, O. P.; Bowler, C.; Friedrich, R.; Cassar, N.; Lin, Y.; John, S. G.; Kelly, R. L.; Cohen, N. R.; Reverdin, G.; Filee, J..
Interactions between the ocean and the atmosphere occur at the air-sea interface through the transfer of momentum, heat, gases and particulate matter, and through the impact of the upper-ocean biology on the composition and radiative properties of this boundary layer. The Tara Pacific expedition, launched in May 2016 aboard the schooner Tara, was a 29-month exploration with the dual goals to study the ecology of reef ecosystems along ecological gradients in the Pacific Ocean and to assess inter-island and open ocean surface plankton and neuston community structures. In addition, key atmospheric properties were measured to study links between the two boundary layer properties. A major challenge for the open ocean sampling was the lack of ship-time available...
Tipo: Text Palavras-chave: Neuston/plankton genomics/taxonomy/imaging; Aerosols; NCP; IOP; Trace metals; Microplastic.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00599/71083/69390.pdf
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Nutrient availability and the ultimate control of the biological carbon pump in the Western Tropical South Pacific Ocean ArchiMer
Moutin, Thierry; Wagener, Thibaut; Caffin, Mathieu; Fumenia, Alain; Gimenez, Audrey; Baklouti, Melika; Bouruet-aubertot, Pascale; Pujo-pay, Mireille; Leblanc, Karine; Lefevre, Dominique; Nunige, Sandra Helias; Leblond, Nathalie; Grosso, Olivier; De Verneil, Alain.
Surface waters (0–200 m) of the western tropical South Pacific (WTSP) were sampled along a longitudinal 4000 km transect (OUTPACE cruise, 18 Feb., 3 Apr. 2015) during the stratified period between the Melanesian Archipelago (MA) and the western part of the SP gyre (WGY). Two distinct areas were considered for the MA, the western MA (WMA) and the eastern MA (EMA). The main carbon (C), nitrogen (N), phosphorus (P) pools and fluxes allow for characterization of the expected trend from oligotrophy to ultra-oligotrophy, and to build first-order budgets at the daily and seasonal scales (using climatology). Sea surface chlorophyll a reflected well the expected oligotrophic gradient with higher values obtained at WMA, lower values at WGY and intermediate values at...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00420/53113/54067.pdf
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Nutrient Fluxes Associated With Submarine Groundwater Discharge From Karstic Coastal Aquifers (Côte Bleue, French Mediterranean Coastline) ArchiMer
Bejannin, Simon; Tamborski, Joseph James; Van Beek, Pieter; Souhaut, Marc; Stieglitz, Thomas; Radakovitch, Olivier; Claude, Christelle; Conan, Pascal; Pujo-pay, Mireille; Crispi, Olivier; Le Roy, Emilie; Estournel, Claude.
Determination of submarine groundwater discharge (SGD) from karstic coastal aquifers is important to constrain hydrological and biogeochemical cycles. However, SGD quantification using commonly employed geochemical methods can be difficult to constrain under the presence of large riverine inputs, and is further complicated by the determination of the karstic groundwater endmember. Here, we investigated a coastal region where groundwater discharges from a karstic aquifer system using airborne thermal infrared mapping and geochemical sampling conducted along offshore transects. We report radium data (223Ra, 224Ra, 228Ra) that we used to derive fluxes (water, nutrients) associated with terrestrial groundwater discharge and/or seawater circulation through the...
Tipo: Text Palavras-chave: Submarine groundwater discharge; Mediterranean sea; Radium isotopes; Thermal infrared remote sensing; Nutrient fluxes; GEOTRACES.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00610/72171/70945.pdf
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Programmed cell death in diazotrophs and the fate of organic matter in the western tropical South Pacific Ocean during the OUTPACE cruise ArchiMer
Spungin, Dina; Belkin, Natalia; Foster, Rachel; Stenegren, Marcus; Caputo, Andrea; Pujo-pay, Mireille; Leblond, Nathalie; Dupouy, Cecile; Bonnet, Sophie; Berman-frank, Ilana.
The fate of diazotroph (N2 fixers) derived carbon (C) and nitrogen (N) and their contribution to vertical export of C and N in the Western Tropical South Pacific Ocean was studied in OUTPACE (Oligotrophy to UlTra-oligotrophy PACific Experiment). Our specific objective during OUTPACE was to determine whether autocatalytic programmed cell death (PCD) is an important mechanism affecting diazotroph mortality and a factor regulating the vertical flux of organic matter and thus the fate of the blooms. We sampled at three long duration (LD) stations of 5 days each (LDA, LDB, and LDC) where drifting sediment traps were deployed at 150, 325 and 500 m depths. LDA and LDB were characterized by high chlorophyll a (Chl a) concentrations (0.2–0.6 µg L−1) and dominated...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00419/53048/53985.pdf
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The Bouraké semi- enclosed lagoon (New Caledonia). A natural laboratory to study the life-long adaptation of a coral reef ecosystem to climate change-like conditions ArchiMer
Maggioni, Federica; Pujo-pay, Mireille; Aucan, Jérôme; Cerrano, Carlo; Calcinai, Barbara; Payri, Claude; Benzoni, Francesca; Letourneur, Yves; Rodolfo-metalpa, Riccardo.
According to current experimental evidence, coral reefs could disappear within the century if CO2 emissions remain unabated. However, recent discoveries of diverse and high cover reefs that already thrive under extreme conditions seem to contradict these projections. Volcanic CO2 vents, semi-enclosed lagoons and mangrove estuaries are unique study sites where one or more ecologically relevant parameters for life in the oceans are close or even worse than currently projected for the year 2100. These natural analogues of future conditions hold new hope for the future of coral reefs and provide unique natural laboratories to explore how reef species could keep pace with climate change. To achieve this, it is essential to characterize their environment as a...
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Ano: 2021 URL: https://archimer.ifremer.fr/doc/00693/80480/83620.pdf
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