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| Butot, Sophie; Le Guyader, Soizick; Krol, Joanna; Putallaz, Thierry; Amoroso, Richard; Sanchez, Gloria. |
| Human noroviruses (NoVs) are the most common viruses causing acute gastroenteritis in humans. Performance characteristics of two commercial quantitative NoV RT-PCR assays, the Norovirus real-time RT-PCR Kit (AnDiaTec) and the Type I and Type II kits (Generon), and the international assay as selected by the CEN/TC/WG6/TAG4 group were evaluated for the specific detection and quantitation of 59 NoV samples, including different subtypes of NoV genogroup I and II. The results showed that the method proposed by the CEN/TC/WG6/TAG4 group was 100% specific since it was able to detect all samples tested. The commercialized kits evaluated failed to detect a vast majority of NoV GI strains. Additionally the Generon kit did not succeed to detect strains from GII.3,... |
| Tipo: Text |
Palavras-chave: Norovirus; Real-time RT-PCR; Commercial kit. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00006/11760/8473.pdf |
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