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Effect of somatotropin on survival and diameter of bovine preantral follicles Arq. Bras. Med. Vet. Zootec.
Silva,T.F.; Costa,S.L.; Costa,E.P.; Guimarães,J.D.; Queiroz-Castro,V.L.D..
ABSTRACT The aim of this study was to evaluate the effect of Recombinant bovine somatotropin (rbST) on survival and diameter of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovaries were collected from adult cows and fragments of ovarian cortex were immediately fixed (non-cultured control) or cultured in vitro in α-MEM+ alone or containing 10, 50, 100 or 1,000ng/mL rbST. The fragments were processed for Classical Histology and Transmission Electron Microscopy. After one and seven days of culture, the percentage of normal follicles in the non-cultured control was superior (P< 0.05) to the follicles cultured in α-MEM+ alone or with different rbST concentrations. The oocyte and follicular mean diameter did not increase during the culture for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; In vitro culture; Somatotropin; Preantral follicles.
Ano: 2019 URL:
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