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CRYOAQUA. Centralisation de la cryoconservation et du stockage des ressources génétiques aquacoles françaises 5
Labbe, Catherine; Suquet, Marc; Michel, Gildas; Duclos, Delphine; Lemarchand, Alain; Le Hellec, Catherine; Vandeputte, Marc; Quittet, Benjamin; Goardon, Lionel; Pincent, Cedric; Haffray, Pierrick.
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00099/20979/18602.pdf
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Cryopreservation of Pacific oyster ( Crassostrea gigas ) larvae: Revisiting the practical limitations and scaling up the procedure for application to hatchery 5
Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc.
Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing...
Tipo: Text Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf
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Genetic parameters of in-vivo prediction of carcass, head and fillet yields by internal ultrasound and 2D external imagery in large rainbow trout (Oncorhynchus mykiss) 5
Haffray, Pierrick; Bugeon, Jerome; Rivard, Quentin; Quittet, Benjamin; Puyo, Sophie; Allamelou, Jean Michel; Vandeputte, Marc; Dupont-nivet, Mathilde.
Selection to improve processing yields relies on sib selection, in which live candidates are ranked according to their family breeding value. This approach limits genetic progress, as it only exploits genetic variability between families and not within them. Indirect criteria measured on live candidates could overcome this limitation. The present study (1) proposes a procedure to identify indirect criteria to predict processing yields in rainbow trout (head, carcass and fillet yields), (2) estimates genetic parameters of these indirect criteria, and (3) predicts relative genetic gains in processing yields using full-sib selection or indirect individual selection on those indirect criteria. DNA-pedigreed all-female rainbow trout Oncorhynchus mykiss (n =...
Tipo: Text Palavras-chave: Salmonids; Heritability; Fillet yield; Selection; Aquaculture; Morphometry.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26847/25249.pdf
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Survival, Growth and Reproduction of Cryopreserved Larvae from a Marine Invertebrate, the Pacific Oyster (Crassostrea gigas) 5
Suquet, Marc; Labbe, Catherine; Puyo, Sophie; Mingant, Christian; Quittet, Benjamin; Boulais, Myrina; Queau, Isabelle; Ratiskol, Dominique; Diss, Blandine; Haffray, Pierrick.
This study is the first demonstration of successful post-thawing development to reproduction stage of diploid cryopreserved larvae in an aquatic invertebrate. Survival, growth and reproductive performances were studied in juvenile and adult Pacific oysters grown from cryopreserved embryos. Cryopreservation was performed at three early stages: trochophore (13 +/- 2 hours post fertilization: hpf), early D-larvae (24 +/- 2 hpf) and late D-larvae (43 +/- 2 hpf). From the beginning (88 days) at the end of the ongrowing phase (195 days), no mortality was recorded and mean body weights did not differ between the thawed oysters and the control. At the end of the growing-out phase (982 days), survival of the oysters cryopreserved at 13 +/- 2 hpf and at 43 +/- 2 hpf...
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00190/30092/28635.pdf
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