|
|
|
Registros recuperados: 18 | |
|
|
PEREIRA, M. M; MOUTON, L.; YÉPRÉMIAN, C.; COUTÉ, A.; LO, J.; MARCONCINI, J. M.; LADEIRA, L. O.; RAPOSO, N. R. B.; BRANDAO, H. de M.; BRAYNER, R.. |
Tipo: Separatas |
Palavras-chave: Bioindicador; Nanopartícula; Nanotoxicologia; Uptake.; Microalga.. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010348 |
| |
|
| |
|
|
MUNK, M.; LADEIRA, L. O.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; RAPOSO, N. R. B.; SERAPIÁO, R. V.; QUINTAO, C. C. R.; SILVA, S. R.; SOARES, J. S.; JORIO, A.; BRANDAO, H. de M.. |
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher... |
Tipo: Separatas |
Palavras-chave: Gene delivery; Pellucid zone.; In vitro fertilization.. |
Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1064560 |
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
POLONINI, H. C.; BRANDAO, H. de M.; RAPOSO, N. R. B.; BRANDÃO, M. A. F.; MOUTON, L.; COUTÉ, A.; YÉPRÉMIAN, C.; SIVRY, Y.; BRAYNER, R.. |
Studies have been demonstrating that smaller particles can lead to unexpected and diverse ecotoxicological effects when compared to those caused by the bulk material. In this study, the chemical composition, size and shape, state of dispersion, and surface's charge, area and physicochemistry of micro (BT MP) and nano barium titanate (BT NP) were determined. Green algae Chlorella vulgaris grown in Bold's Basal (BB) medium or Seine River water (SRW) was used as biological indicator to assess their aquatic toxicology. Responses such as growth inhibition, cell viability, superoxide dismutase (SOD) activity, adenosine-5-triphosphate (ATP) content and photosynthetic activity were evaluated. Tetragonal BT (~170 nm, 3.24 m(2) g(-1) surface area) and cubic BT (~60... |
Tipo: Separatas |
Palavras-chave: Seine river water; Characterization.; Barium titanate; Ecotoxicology; Nanotechnology.. |
Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037622 |
| |
|
| |
Registros recuperados: 18 | |
|
|
|