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An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. Repositório Alice
OLIVEIRA, P. S. N. de; COUTINHO, L. L.; TIZIOTO, P. C.; CESAR, A. S. M.; OLIVEIRA, G. B. de; DINIZ, W, J. da S.; LIMA, A. O. de; REECY, J. M.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REGITANO, L. C. de A..
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21,...
Tipo: Artigo de periódico Palavras-chave: RFI; MiRNA; Gado Nelore; Beef cattle; Nellore; Skeletal muscle.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099755
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An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. Repositório Alice
OLIVEIRA, P. S. N. de; COUTINHO, L. L.; TIZIOTO, P. C.; CESAR, A. S. M.; OLIVEIRA, G. B. de; DINIZ, W, J. da S.; LIMA, A. O. de; REECY, J. M.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REGITANO, L. C. de A..
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21,...
Tipo: Artigo de periódico Palavras-chave: MicroRNAs; Residual Feed Intake; Gado Nelore; Beef cattle; Nellore; Skeletal muscle.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102149
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Association of skeletal muscle transcripts with fatty acid content in Nellore cattle. Repositório Alice
CESAR, A. S. M.; REGITANO, L. C. de A.; POLETI, M. D.; ANDRADE, S. C. da S.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; LANNA, D. P. D.; TULLIO, R. R.; NASSU, R. T.; KOLTES, J. E.; WATERS, E. F.; REECY, J. M.; COUTINHO, L. L..
Tipo: Separatas Palavras-chave: Differentially expressed genes.; Conjugated linoleic acid; Oleic acid..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1060623
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Co-expression networks reveal potential regulatory roles of miRNAs in fatty acid composition of nelore cattle. Repositório Alice
OLIVEIRA, P. S. N. de; COUTINHO, L. L.; CESAR, A. S. M.; DINIZ, W. J. da S.; SOUZA, M. de S.; ANDRADE, B. G.; KOLTES, J. E.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; REGITANO, L. C. de A..
Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were...
Tipo: Artigo de periódico Palavras-chave: Ácido linoleico conjugado; Ácido oleico; Ácidos graxos; Genômica; Redes de co-expressão; Integrative genomics; MRNA; MiRNA; Bos Indicus; Gado de Corte; Beef cattle; Conjugated linoleic acid; Oleic acid.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110820
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Comparative untargeted metabolome analysis of ruminal fuid and feces of Nelore steers (Bos indicus). Repositório Alice
MALHEIROS, J. M.; CORREIA, B. S. B.; CERIBELI, C.; CARDOSO, D. R.; COLNAGO, L. A.; BOGUSZ JUNIOR, S.; REECY, J. M.; MOURÃO, G. B.; COUTINHO, L. L.; PALHARES, J. C. P.; BERNDT, A.; REGITANO, L. C. de A..
We conducted a study to identify the fecal metabolite profle and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fuid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented signifcantly higher concentrations of the polar compounds when compared to feces (P< 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fuid metabolites. All fatty acids present in the ruminal fuid were also...
Tipo: Artigo de periódico Palavras-chave: Metabolomics analysis; Fecal metabolites.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1132495
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Comparative untargeted metabolome analysis of ruminal fuid and feces of Nelore steers (Bos indicus). Repositório Alice
MALHEIROS, J. M.; CORREIA, B. S. B.; CERIBELI, C.; CARDOSO, D. R.; COLNAGO, L. A.; BOGUSZ JUNIOR, S.; REECY, J. M.; MOURÃO, G. B.; COUTINHO, L. L.; PALHARES, J. C. P.; BERNDT, A.; REGITANO, L. C. de A..
We conducted a study to identify the fecal metabolite profle and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fuid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented signifcantly higher concentrations of the polar compounds when compared to feces (P< 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fuid metabolites. All fatty acids present in the ruminal fuid were also...
Tipo: Artigo de periódico Palavras-chave: Fecal metabolite profile; Ruminal metabolism of Nelore; Rumen fluid; Acetate; Propionate; Butyrate; Degradation of ketone bodies; Aspartate; Glutamate; Alanine; Animal production.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137652
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Detection of quantitative trait loci for mineral content of Nelore longissimus dorsi muscle Repositório Alice
TIZIOTO, P. C.; TAYLOR, J. F.; DECKER, J. E.; GROMBONI, C. F.; MUDADU, M. de A.; SCHNABEL, R. D.; COUTINHO, L. L.; MOURÃO, G. B.; OLIVEIRA, P. S. N.; SOUZA, M. M.; REECY, J. M.; NASSU, R. T.; BRESSANI, F. A.; THOLON, P.; SONSTEGARD, T. S.; ALENCAR, M. M. de; TULLIO, R. R.; NOGUEIRA, A. R. de A.; REGITANO, L. C. de A..
Genome-wide association studies (GWAS) can be used to identify quantitative trait loci (QTL) associated with complex traits and to better understand which genes and biological mechanisms underlie phenotypic variation.
Tipo: Artigo de periódico Palavras-chave: Trait loci; Race Nellore; Longissimus dorsi; Mineral.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1017292
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Differences in the skeletal muscle transcriptome profile associated with extreme values of fatty acids content. Repositório Alice
CESAR, A. S. M.; REGITANO, L. C. de A.; POLETI, M. D.; ANDRADE, S. C. da S.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; FELICIO, A. M.; NASCIMENTO, M. L. do; CHAVES, A. S.; LANNA, D. P. D.; TULLIO, R. R.; NASSU, R. T.; KOLTES, J. E.; WATERS, E. F.; MOURAO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; COUTINHO, L. L..
Tipo: Artigo de periódico Palavras-chave: Bos Indicus; Human health; Lipids.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1060542
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Effects of quantitative trait loci on iron content of bovine longissimus dorsi muscle. Repositório Alice
TIZIOTO, P. C.; TAYLOR, J. F.; DECKER, J. E.; GROMBONI, C. F.; MUDADU, M. de A.; SCHNABEL, R. D.; COUTINHO, L. L.; MOURÃO, G. B.; NASSU, R. T.; DONATONI, F. A. B.; THOLON, P.; SONSTEGARD, T. S.; ALENCAR, M. M. de; TULLIO, R. R.; REECY, J. M.; NOGUEIRA, A. R. de A.; REGITANO, L. C. de A..
Tipo: Separatas Palavras-chave: Fe; GWAS.; Bos Indicus..
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/997999
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Expression quantitative trait loci (eQTL) hotspot regions from whole genome analysis of Nellore steers. Repositório Alice
CESAR, A. S. M.; REGITANO, L. C. de A.; LANNA, D. P. D.; POLETI, M. D.; KOLTES, J. E.; REECY, J. M.; FRITZ-WATERS, E.; KRAMER, L.; GARRICK, D.; MUDADU, M. de A.; TULLIO, R. R.; COUTINHO, L. L..
Tipo: Anais e Proceedings de eventos Palavras-chave: Nelore.; Gado nelore; Gado de Corte.; Nellore.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057056
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Gene co-expression analysis indicates potential pathways and regulators of beef tenderness in Nellore cattle. Repositório Alice
GONÇALVES, T. M.; REGITANO, L. C. de A.; KOLTES, J. E.; CESAR, A. S. M.; ANDRADE, S. C. da S.; MOURAO, G. B.; GASPARIN, G.; MOREIRA, G. C. M.; FRITZ-WATERS, E.; REECY, J. M.; COUTINHO, L. L..
Beef tenderness, a complex trait affected by many factors, is economically important to beef quality, industry, and consumer?s palatability. In this study, RNA-Seq was used in network analysis to better understand the biological processes that lead to differences in beef tenderness. Skeletal muscle transcriptional profiles from 24 Nellore steers, selected by extreme estimated breeding values (EBVs) for shear force after 14 days of aging, were analyzed and 22 differentially expressed transcripts were identified. Among these were genes encoding ribosomal proteins, glutathione transporter ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4), and synaptotagmin IV (SYT4). Complementary co-expression analyses using Partial Correlation with Information...
Tipo: Artigo de periódico Palavras-chave: Qualidade da carne; Maciez da carne; Carne; Gado de Corte; Beef; Beef cattle; Beef quality.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106256
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Genome-wide association study for intramuscular fat deposition and composition in Nellore cattle. Repositório Alice
CESAR, A. S. M.; REGITANO, L. C. de A.; MOURÃO, G. B.; TULLIO, R. R.; LANNA, D. P. D.; NASSU, R. T.; MUDADU, M. de A.; OLIVEIRA, P. S. N.; NASCIMENTO, M. L. do; CHAVES, A. S.; ALENCAR, M. M. de; SONSTEGRAD, T. S.; GARRICK, D. J.; REECY, J. M.; COUTINHO, L. L..
Meat from Bos taurus and Bos indicus breeds are an important source of nutrients for humans and intramuscular fat (IMF) influences its flavor, nutritional value and impacts human health. Human consumption of fat that contains high levels of onounsaturated fatt y acids (MUFA) can reduce the concentration of undesirable cholesterol (LDL) in circulating blood. Different feeding practices and genetic variation within and between breeds influences the amount of IMF and fatty acid (FA) composition in meat. However, it is difficult and costly to determine fatty acid composition, which has precluded beef cattle breeding programs from selecting for a healthier fatty acid profile. In this study, we employed a high-density single nucleotide polymorphism (SNP) chip to...
Tipo: Artigo de periódico Palavras-chave: Fatty acid; GWAS; Positional candidate gene; Bos Indicus; Beef.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/984717
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Identification of genomic regions associated with feed efficiency in Nelore cattle. Repositório Alice
OLIVEIRA, P. S. N.; CESAR, A. S. M.; NASCIMENTO, M. L. do; CHAVES, A. S.; TIZIOTO, P. C.; TULLIO, R. R.; LANNA, D. P. D.; ROSA, A. do N.; SONSTEGARD, T. S.; MOURÃO, G. B.; REECY, J. M.; GARRICK, D. J.; MUDADU, M. de A.; COUTINHO, L. L.; REGITANO, L. C. de A..
Feed efficiency is jointly determined by productivity and feed requirements, both of which are economically relevant traits in beef cattle production systems. The objective of this study was to identify genes/QTLs associated with components of feed efficiency in Nelore cattle using Illumina BovineHD BeadChip (770 k SNP) genotypes from 593 Nelore steers. The traits analyzed included: average daily gain (ADG), dry matter intake (DMI), feed-conversion ratio (FCR), feed efficiency (FE), residual feed intake (RFI), maintenance efficiency (ME), efficiency of gain (EG), partial efficiency of growth (PEG) and relative growth rate (RGR). The Bayes B analysis was completed with Gensel software parameterized to fit fewer markers than animals. Genomic windows...
Tipo: Artigo de periódico Palavras-chave: Candidate gene; Residual feed intake; Bos Indicus; Single nucleotide polymorphism.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002799
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Identification of putative regulatory regions and transcription factors associated with intramuscular fat content traits. Repositório Alice
CESAR, A. S. M.; REGITANO, L. C. de A.; REECY, J. M.; POLETI, M. D.; OLIVEIRA, P. S. N.; OLIVEIRA, G. B. de; MOREIRA, G. C. M.; MUDADU, M. de A.; TIZIOTO, P. L.; KOLTES, J. E.; Fritz-Waters, E.; KRAMER, L.; GARRICK, D.; BEIKI, H.; GEISTLINGER, L.; MOURÃO, G. B.; ZERLOTINI NETO, A.; COUTINHO, L. L..
Background: Integration of high throughput DNA genotyping and RNA-sequencing data allows for the identification of genomic regions that control gene expression, known as expression quantitative trait loci (eQTL), on a whole genome scale. Intramuscular fat (IMF) content and carcass composition play important roles in metabolic and physiological processes in mammals because they influence insulin sensitivity and consequently prevalence of metabolic diseases such as obesity and type 2 diabetes. However, limited information is available on the genetic variants and mechanisms associated with IMF deposition in mammals. Thus, our hypothesis was that eQTL analyses could identify putative regulatory regions and transcription factors (TFs) associated with...
Tipo: Artigo de periódico Palavras-chave: Expressão gênica; EQTL; Ácidos graxos; Doenças metabólicas; Expression quantitative trait loci; Gene expression; Fatty acids; Mammals; Metabolic diseases.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102203
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Integration of GWAS, CNV and sele ction signature reveals candidate genes for abdominal fat regulation in chickens. Repositório Alice
MOREIRA, G. C. M.; GODOY, T. F.; BOSCHIERO, C.; CESAR, A. S. M.; REECY, J. M.; LEDUR, M. C.; GARRICK, D.; SILVA, V. H. da; CROOIJAMANS, R. P.; GROENEN, M. A. M.; COUTINHO, L. L..
Abstract: Carcass fat content is an economically important trait in commercial chickens. The use of genome-wide high density SNPs may improve the power and resolution to identify QTLs, putative candidate genes and copy number variations (CNVs), for selection programs. The main goal of this study was to identify genomic windows and putative candidate genes for carcass fat content. We checked the overlap of QTL with regions demonstrating signatures of selection and inherited CNVs identified in the same population. A total of 497 42 day-old chickens from the EMBRAPA F2 Chicken Resource Population developed for QTL studies were genotyped with the 600K SNP genotyping array (Affymetrix®), and phenotyped for carcass fat content weight (CFCW) and carcass fat...
Tipo: Separatas Palavras-chave: Genotyping by sequencing; Analyses were performed with GenSel; Selection signatures; 600k snp genotyping array; Melhoramento Genético Animal; Polimorfismo Genético; Frango de Corte; Carcaça; Gordura; Genoma; Animal genetic resources; Broiler chickens; Carcass characteristics; Abdominal fat; Genetic polymorphism; Single nucleotide polymorphism.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093261
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miRNAs related to fatty acids composition in Nellore cattle. Repositório Alice
OLIVEIRA, P. S. N. de; CESAR, A. S. M.; OLIVEIRA, G. B. de; TIZIOTO, P. C.; POLETI, M. D.; DINIZ, W. J. da S.; LIMA, A. O. de; REECY, J. M.; COUTINHO, L. L.; REGITANO, L. C. de A..
Tipo: Separatas Palavras-chave: Gene regulation.; Bos Indicus.; Adipogenesis..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1066405
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Positional candidate genes for residual intake and gain in Nelore beef cattle. Repositório Alice
OLIVEIRA, P. S. N.; CESAR, A. S. M.; NASCIMENTO, M. L. do; SOUZA, M. M.; TULLIO, R. R.; LANNA, D. P.; SONSTEGARD, T.; MOURÃO, G. B.; REECY, J. M.; GARRICK, D. J.; MUDADU, M. de A.; REGITANO, L. C. de A.; COUTINHO, L. L..
Tipo: Separatas Palavras-chave: Feed efficiency; SNPs.; Bos Indicus..
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/998065
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Stool and ruminal microbiome components associated with methane emission and feed efficiency in Nelore beef cattle. Repositório Alice
ANDRADE, B. G. N.; DONATONI, F. A. B.; CUADRAT, R. R.; CARDOSO, T. F.; MALHEIROS, J. M.; OLIVEIRA, P. S. N. DE; PETRINI, J.; MOURÃO, G. B.; COUTINHO, L. L.; REECY, J. M.; KOLTES, J. E.; ZERLOTINI NETO, A.; MEDEIROS, S. R. de; BERNDT, A.; PALHARES, J. C. P.; AFLI, H.; REGITANO, L. C. de A..
Background: The impact of extreme changes in weather patterns on the economy and human welfare is one of the biggest challenges our civilization faces. From anthropogenic contributions to climate change, reducing the impact of farming activities is a priority since it is responsible for up to 18% of global greenhouse gas emissions. To this end, we tested whether ruminal and stool microbiome components could be used as biomarkers for methane emission and feed efficiency in bovine by studying 52 Brazilian Nelore bulls belonging to two feed intervention treatment groups, that is, conventional and by-product-based diets. Results: We identified a total of 5,693 amplicon sequence variants (ASVs) in the Nelore bulls? microbiomes. A Differential abundance analysis...
Tipo: Artigo de periódico Palavras-chave: Association; Feed efficiency; Methane emission; Bactéria; Bos Indicus; Archaea; Biomarkers.
Ano: 2022 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1145925
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The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen. Repositório Alice
ANDRADE, B. G. N.; DONATONI, F. A. B.; CUADRAT, R. R. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; MOURÃO, G. B.; COUTINHO, L. L.; REECY, J. M.; KOLTES, J. E.; WALSH, P.; BERNDT, A.; PALHARES, J. C. P.; REGITANO, L. C. de A..
The success of different species of ruminants in the colonization of a diverse range of environments is due to their ability to digest and absorb nutrients from cellulose, a complex polysaccharide found in leaves and grass. Ruminants rely on a complex and diverse microbial community, or microbiota, in a unique compartment known as the rumen to break down this polysaccharide. Changes in microbial populations of the rumen can affect the host?s development, health, and productivity. However, accessing the rumen is stressful for the animal. Therefore, the development and use of alternative sampling methods are needed if this technique is to be routinely used in cattle breeding. To this end, we tested if the fecal microbiome could be used as a proxy for the...
Tipo: Artigo de periódico Palavras-chave: Microbiota; Metabarcoding; Bactéria; Bos Indicus; Gado Nelore; Archaea; Methanobrevibacter; Microbiome.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121594
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Transcriptome profile in Longissimus dorsi muscle from Nellore steers with extreme GEBV for intramuscular fat percentage. Repositório Alice
CESAR, A. S. M.; REGITANO, L. C. de A.; KOLTES, J.; FRITZ-WATERS, E. R.; TULLIO, R. R.; LANNA, D. P. D.; REECY, J. M.; MOURÃO, G. B.; GASPARIN, G.; COUTINHO, L. L..
Tipo: Separatas Palavras-chave: GEBV; Transcriptome profile.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/988784
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