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Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean. Repositório Alice
STOLF-MOREIRA, R.; LEMOS, E. G. de M.; ABDELNOOR, R. V.; BENEVENTI, M. A.; ROLLA, A. A. P.; PEREIRA, S. dos S.; OLIVEIRA, M. C. N. de; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C..
The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: GmB-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to...
Tipo: Artigo de periódico Palavras-chave: Estabilidade de expressão; Expressão gênica; RT-qPCR; Soja; Biotecnologia; Deficiência hídrica; Soybeans; Gene expression; Plant-water relations; Agricultural biotechnology.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/882641
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Introdução de genes por biobalística em soja visando tolerância à seca. Repositório Alice
NEPOMUCENO, A. L.; FARIAS, J. R. B.; YAMAGUCHI-SHINOZAKI, K.; BENEVENTI, M. A.; NEUMAIER, N.; YAMANAKA, N.; NAKASHIMA, K.; BINNECK, E.; MARIN, S. R. R.; STOLF, R.; FUGANTI, R.; SILVEIRA, C. A. da; OLIVEIRA, M. C. N. de; ABDELNOOR, R. V.; ROLLA, A. A. P.; POLIZEL, A. M..
Tipo: Parte de livro Palavras-chave: Estresse abiótico; Aquecimento global; Elevação de temperatura; Soja; Resistência à seca; Condição ambiental; Climatologia; Deficiência hídrica; Clima; Produção agrícola; Soybeans; Drought tolerance.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/469118
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Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance. Repositório Alice
POLIZEL, A. M.; MEDRI, M. E.; NAKASHIMA, K.; YAMANAKA, N.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; MARIN, S. R. R.; ABDELNOOR, R. V.; MARCELINO, F. C.; FUGANTI, R.; RODRIGUES, F. A.; STOLF-MOREIRA, R.; BENEVENTI, M. A.; ROLLA, A. A. P.; NEUMAIER, N.; YAMAGUCHI-SHINOZAKI, K.; CARVALHO, J. F. C.; NEPOMUCENO, A. L..
We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four randomized blocks of soybean plants, with each treatment performed in triplicate. Seeds from the non-transformed soybean cultivar BR16 and from the genetically modified soybean P58 line (T2 generation) were grown at 15% gravimetric humidity for 31 days. To induce water deficit, the humidity was reduced to 5% gravimetric humidity (moderate stress) for 29 days and then to 2.5% gravimetric humidity (severe stress). AtDREB1A gene expression...
Tipo: Separatas Palavras-chave: Soja; Resistência a seca; Fisiologia vegetal; Soybeans; Drought tolerance; Plant physiology.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/916042
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Molecular characterization of genetically modified soybean lines developed aiming to obtain drought tolerance. Repositório Alice
ROLLA, A. A. P.; KANAMORI, N.; FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; ENGLES, C.; YAMAGUCHI-SHINOZAKI; FARIAS, J. R. B.; NEUMAIER, N.; ABDELNOOR, R. V.; MARCELINO, F. C.; NEPOMUCENO, A. L..
Tipo: Parte de livro Palavras-chave: Gene expression..
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/913815
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Obtenção de plantas de soja geneticamente modificadas via Agrobacterium tumefaciens. Repositório Alice
LEITE, J. P.; KANAMORI, N.; FUGANTI, R.; GIROTTO, L.; ROLLA, A. A. P.; ENGELS, C.; MARINHO, J. P.; FARIAS, J. R. B.; NEUMAIER, N.; ABDELNOOR, R. V.; MARCELINO, F. C.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L..
Tipo: Parte de livro Palavras-chave: Soja; Planta transgênica; Resistência à seca; Soybeans; Transgenic plants; Drought tolerance.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/574898
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Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis. Repositório Alice
GOMES, D. F.; BATISTA, J. S. S.; ROLLA, A. A. P.; SILVA, L. P. da; TARASAWA, L. V. G.; HUNGRIA, M..
Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available.
Tipo: Separatas Palavras-chave: Two-dimensional proteomics; RT-qPCR; Bradyrhizobium; Nitrogen fixation; Symbiosis.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/997388
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Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis Repositório Alice
GOMES, D. F.; BATISTA, J. S. da S.; ROLLA, A. A. P.; SILVA, L. P. da; BLOCH, C.; GALLI-TARASAWA, L. V.; HUNGRIA, M..
Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol...
Tipo: Separatas Palavras-chave: Two-dimensional proteomics; RT-qPCR.; Bradyrhizobium; Nitrogen fixation; Symbiosis..
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/993458
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Transcription factors expressed in soybean roots under drought stress. Repositório Alice
PEREIRA, S. S.; GUIMARÃES, F. C. M.; CARVALHO, J. F. C.; STOLF-MOREIRA, R.; OLIVEIRA, M. C. N.; ROLLA, A. A. P.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L..
To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcription factors and analyzed their expression levels under drought stress. The NCBI software was used to find the predicted amino acid sequences of the transcription factors, and the Clustal X software was used to align soybean and other plant species sequences. Phylogenetic trees were built using the Mega 4.1 software by neighbor joining and the degree of confidence test by Bootstrap. Expression level studies were carried out using...
Tipo: Separatas Palavras-chave: Soja; Resistência a seca; Soybeans; Drought tolerance.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/916065
Registros recuperados: 8
Primeira ... 1 ... Última
 

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