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Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil Trop. Plant Pathol.
Dianese,Érico de C.; Ramalho,Eduardo D.; Cerqueira,Daniela M.; Lopes,Daniela B.; Fajardo,Thor V.M.; Ferreira,Marisa A.S.V.; Martins,Cláudia R.F..
Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GLRaV-3; Vitis spp.; Ampelovirus; RNA dependent RNA polymerase.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200012
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