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Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in two metropolitan areas of São Paulo State, southeast Brazil 55
Cury,Gisele Gentile; Mobilon,Cristiane; Stehling,Eliana Guedes; Lancellotti,Marcelo; Ramos,Marcelo de Carvalho; Martinez,Roberto; Brocchi,Marcelo; Silveira,Wanderley Dias da.
One hundred and fifty-one methicillin-resistant z (MRSA) strains have been isolated from patients admitted in tertiary care hospitals in two metropolitan areas (Campinas City and Ribeirão Preto City) in the southeast region of Brazil and analyzed through PCR-based techniques [(PCR amplification of spa, coa, and housekeeping genes (arcC, aroE, gmk, pta, tpi, yqiL)] and further restriction fragment typing of coa and of housekeeping genes. The heterogeneity of spa gene was determined directly by agarose gel electrophoresis migration. The results obtained indicate the existence of three (A, B, C) main clusters. Since the strain distribution in these three clusters is much characteristic, it denotes the existence of three main clones. All strains isolated in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Nosocomial; Molecular characterization; PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300002
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Serum markers as an aid in the diagnosis of pulmonary fungal infections in AIDS patients 55
Passos,Ana Isabela Morsch; Dertkigil,Rachel Polo; Ramos,Marcelo de Carvalho; Busso-Lopes,Ariane Fidelis; Tararan,Cibele; Ribeiro,Erivan Olinda; Schreiber,Angélica Zaninelli; Trabasso,Plinio; Resende,Mariangela Ribeiro; Moretti,Maria Luiza.
ABSTRACT Introduction: The etiology of pulmonary infections in HIV patients is determined by several variables including geographic region and availability of antiretroviral therapy. Materials and methods: A cross-sectional prospective study was conducted from 2012 to 2016 to evaluate the occurrence of pulmonary fungal infection in HIV-patients hospitalized due to pulmonary infections. Patients’ serums were tested for (1-3)-β-D-Glugan, galactomannan, and lactate dehydrogenase. The association among the variables was analyzed by univariate and multivariate regression analysis. Results: 60 patients were included in the study. The patients were classified in three groups: Pneumocystis jirovecii pneumonia (19 patients), community-acquired pneumonia (18...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pulmonary infection; HIV/AIDS; (1-3)-β-D-Glugan; LDH; LAMP; Pneumocystosis; Fungal infection.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600606
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