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| Registros recuperados: 32 | |
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| Heymann, Dominique; Ruiz-velasco, Carmen; Chesneau, Julie; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia. |
| Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no... |
| Tipo: Text |
Palavras-chave: Exopolysaccharides; Glycosaminoglycan; Heparin-like; Derivatives; Sulfation; Bone metabolism; Bone remodeling; Lung mestatases; Osteosarcoma. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00333/44442/44112.pdf |
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| Faury, Nicole; Ratiskol, Jacqueline; Ratiskol, Gilles. |
| La surveillance bactériologique de 1989 à 1992 du bassin de Marennes-Oléron et de la rive droite de la Gironde met en évidence la bonne qualité du milieu. Les dépassements de la norme (>300 coliformes fécaux/100 g de chair et liquide intervalvaire) sont peu fréquents. Quelques pollutions sporadiques peuvent apparaître, le plus souvent en été, et sont liées au dysfonctionnement passager des systèmes d'assainissement (postes de relèvement, stations d'épuration) ou aux infiltrations d'eaux usées dans les réseaux pluviaux. L'application de la directive européenne de 1991 aurait une incidence limitée sur le classement des zones de production conchylicoles du bassin de Marennes-Oléron. |
| Tipo: Text |
Palavras-chave: Bactériologie; Surveillance; Coliformes fécaux; Marennes Oléron; Gironde. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00014/12497/9344.pdf |
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| Colliec-jouault, Sylvia; Chevolot, Lionel; Helley, Dominique; Ratiskol, Jacqueline; Bros, Andrée; Sinquin, Corinne; Roger, Olivier; Fischer, Anne-marie. |
| A new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. Data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. Two depolymerization processes were used to obtain low-molecular-weight (24-35 x 10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%), Nuclear magnetic resonance studies indicated that after sulfation (40%), the low-molecular-weight fraction obtained by free radical depolymerization was less sulfated in the 6-O-position than the fraction depolymerized by acid hydrolysis. The free radical depolymerized product also had sulfated residues in... |
| Tipo: Text |
Palavras-chave: Sulfation; Sulfated polysaccharide; Serpin; Heparin like; Depolymerization; Anticoagulant activity; Affinity co electrophoresis. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-2111.pdf |
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| Heurtebise, Serge; Ratiskol, Jacqueline. |
| Comme précédemment en 1985 et 1986, le suivi de la reproduction des huîtres et des moules a été assuré par le CSRU de La Tremblade pour les secteurs clefs de la production du naissain que sont l'embouchure de la Charente, la Seudre et le bassin de Marennes-Oléron. Ce suivi a nécessité 40 sorties en mer, dont 21 à partir du Chapus ou de La Tremblade (moyens nautiques IFREMER) et 19 au départ de Port des Barques avec la location des services d'un professionnel. Les différents paramètres physico-chimiques (T°, S %, 02) ont été mesurés à l'occasion de chaque pêche de larves pour appréhender au mieux l'évolution de la reproduction et fournir des indications complémentaires sur les bulletins de numération. Cette année, 30 bulletins sont parvenus à la profession... |
| Tipo: Text |
Palavras-chave: Reproduction; Larves; Crassostrea gigas; Mytilus edulis; Bassin de Marennes Oléron. |
| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00049/16071/13533.pdf |
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| Matou, Sabine; Colliec-jouault, Sylvia; Galy Fauroux, Isabelle; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, Anne-marie; Helley, Dominique. |
| The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6)... |
| Tipo: Text |
Palavras-chave: Matrigel; Collagen gel; Alpha 6 integrin subunit; Angiogenic growth factor; Polysaccharide; Angiogenesis. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2118.pdf |
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| Faury, Nicole; Ratiskol, Jacqueline. |
| La décontamination bactériologique des huitres Crassostrea gigas a été étudiée expérimentalement dans des bassins dégorgeoirs de 13 m3 de conception proche de la configuration normale d'une station de purification française. Les huîtres contaminées artificiellement ont été placées dans des conditions dites "naturelles" d'épuration. Il n'a pas été utilisé de procédé physique ou chimique de purification de l'eau. Elles ont été soumises sur trois périodes de l'année à trois conditions différentes de renouvellement d'eau : quotidien, biquotidien ou apport continu, le témoin étant caractérisé par l'absence de tout renouvellement d'eau. Les résultats permettent de dégager un ensemble de remarques sur l'état physiologique, sur l'activité métabolique des... |
| Tipo: Text |
Palavras-chave: Décontamination; Coliformes fécaux; Streptocoques fécaux; Bassins; Purification. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00050/16156/13639.pdf |
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| Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean. |
| Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new... |
| Tipo: Text |
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf |
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| Petit, Anne-celine; Noiret, N; Sinquin, Corinne; Ratiskol, Jacqueline; Guezennec, Jean; Colliec-jouault, Sylvia. |
| Free-radical depolymerization with metallic catalysts has been investigated to depolymerize an exopolysaccharide (EPS), with a high molecular weight (> 10(6) g/Mol), produced by the bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. Three metals (copper, zinc and manganese) were used either alone or mixed. These metals led to the depolymerization of the high molecular weight bacterial polysaccharide with different efficiency. This study specifies some conditions required to produce, with good reproducibility and yield, EPS derivatives of reduced molecular weight ranging from 100,000 to 20,000 g/mol. The more influential parameters are specified. This is a first study to find suitable industrial conditions in the presence of these metallic... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharide; Free radical depolymerization; H202; Metal. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2146.pdf |
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| Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine. |
| Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the... |
| Tipo: Text |
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf |
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| Faury, Nicole; Razet, Daniel; Soletchnik, Patrick; Goulletquer, Philippe; Ratiskol, Jacqueline; Garnier, Jacqueline. |
| An hydrobiological monitoring survey has been carried out in the Bay of Marennes-Oléron since 1977. Five stations, distributed over the Bay, were sam pied twice a month. Spatial and temporal trends of the physical characteristics (i.e., seawater, temperature, salinity, dissolved oxygen and suspended matter), nutrients (azote, nitrite, nitrate, silicate) and organic variables (chlorophyll a and pheopigments) were assessed by using a statistical time-series analysis mode\. It was used to distinguish seasonal variability from long term trends. Pluviometric data as weil as the Charente River fluxes were similary analyzed by the model. The seasonal variability showed an homogeneous pattern over the five stations for all parameters, but the site-dependent... |
| Tipo: Text |
Palavras-chave: Shellfish farming; Overall trends; Seawater seasonal variability; Marennes Oleron Bay; Hydrology; Conchyliculture; Tendances; Variations saisonnières; Marennes Oléron; Hydrologie. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/rapport-6379.pdf |
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| Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline. |
| Les agriculteurs aménageant quelques centaines d'hectares du marais doux de Hiers Brouage (mise à plat, drainage) afin de le mettre en culture, il était important de pouvoir mesurer l'impact que peuvent avoir les rejets de drainage-pompage dans le milieu marin par l'intermédiaire des chenaux ostréicoles. Ceci afin d'éviter d'éventuels conflits entre groupes socio-professionnels (agriculteurs et conchyliculteurs) en proposant des mesures et de solutions. Un suivi est donc effectué à la demande des administrations, en collaboration avec l'INRA et le CEMAGREF, lesquels assurent le suivi de la lagune de décantation des eaux de drainage. L'IFREMER prenant en compte le milieu récepteur (chenaux du Grand Garçon et de Brouage). A raison de cinq sériées de... |
| Tipo: Text |
Palavras-chave: Qualité des eaux; Agriculture; Rejets; Marais maritimes; Chenal de Brouage; Charente-Maritime. |
| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00087/19850/17500.pdf |
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| Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline. |
| L'étude entreprise en 1985 sur la qualité des eaux de rejet de la zone cultivée de l'Ilot des Tannes, dans le marais de Moeze, examine maintenant les résultats de la deuxième année de culture intensive. Rappelons que cette étude a pour but d'apprécier la qualité physico-chimique des eaux de ruissellement et le drainage arrivant dans le milieu ostréicole par l'intermédiaire du Chenal de Brouage. Les compagnes des deux années précédentes après un point zéro et une première année de mise en culture avaient fait apparaître, outre la présence d'insecticides rémanents, une dominance qualitative et quantitative des herbicides aussi bien dans l'eau que dans la matière vivante. Chaque année, des difficultés techniques différentes limitent l'information recueillie,... |
| Tipo: Text |
Palavras-chave: Qualité des eaux; Marais Maritime; Agriculture; Rejets agricoles; Chenal de Brouage; Charente-Maritime. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/00087/19849/17499.pdf |
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| Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline. |
| Le bassin de Marennes Oléron, comme toutes les zones conchylicoles, est susceptible d'être touché par les apports pollués. Dans ce domaine, le vecteur potentiel que constitue la Charente méritait d'être étudié plus particulièrement, la qualité bactériologique des eaux pouvant affecter par la suite la salubrité des coquillages. L'étude précédente (Masson et al. 1988) a fait apparaître que la pollution bactérienne apportée par la Charente (particulièrement en étiage) sédimentait rapidement, la quantité de germes devenant très faible à la sortie de l'estuaire. Toutefois, comme l'enjeu économique est important (perspectives européennes) il était nécessaire de confirmer ce résultat avec un système de suivi identique à celui utilisé en 1987. De plus, un certain... |
| Tipo: Text |
Palavras-chave: Bactériologie; Qualité eaux; Salubrité coquillages; Pollution bactérienne; Estuaire; Charente; Charente Maritime Dep.. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00087/19827/17469.pdf |
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| Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia. |
| The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the... |
| Tipo: Text |
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf |
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| Roger, Olivier; Colliec-jouault, Sylvia; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, A; Chevolot, Lionel. |
| Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science... |
| Tipo: Text |
Palavras-chave: Anticoagulant activity; Reductive amination; Fluorescent tagging; Fucan; GAG; Sulphated polysaccharide. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2113.pdf |
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| Registros recuperados: 32 | |
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