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Registros recuperados: 7
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Cloning and characterization of a tyrosinase gene from the white-rot fungus Pycnoporus sanguineus, and overproduction of the recombinant protein in Aspergillus niger Inra
Halaouli, S.; Record, E.; Casalot, L.; Hamdi, M.; Sigoillot, J.C.; Lomascolo, A.; Asther, M..
A new tyrosinase-encoding gene (2,204 bp) andthe corresponding cDNA (1,857 nucleotides) from thewhite-rot fungus Pycnoporus sanguineus BRFM49 werecloned. This gene consisted of seven exons and six intronsand encoded a predicted protein of 68 kDa, exceeding themature tyrosinase by 23 kDa. P. sanguineus tyrosinasecDNA was over-expressed in Aspergillus niger, a particularlysuitable fungus for heterologous expression of proteinsof biotechnological interest, under the control ofthe glyceraldehyde-3-phosphate-dehydrogenase promoteras strong and constitutive promoter. The glucoamylasepreprosequence of A. niger was used to target the secretion.This construction enabled the production of recombinanttyrosinase in the extracellular medium of A. niger. Theidentity of...
Tipo: Journal Article Palavras-chave: FUNGAL TYROSINASE.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20078d7fe834&uri=/notices/prodinra1/2010/09/
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Comparison of the characteristics of fungal and plant tyrosinases Inra
Selinheimo, E.; NiEidhin, D.; Steffensen, C.; Nielsen, J.; Lomascolo, A.; Halaouli, S.; Record, E.; O’Beirne, D.; Buchert, J.; Kruus, K..
Enzymatic crosslinking provides valuable means for modifying functionality and structural properties of different polymers. Tyrosinases catalyze the hydroxylation of various monophenols to the corresponding o-diphenols, and the subsequent oxidation of o-diphenols to the corresponding quinones, which are highly reactive and can further undergo non-enzymatic reactions to produce mixed melanins and heterogeneous polymers. Tyrosinases are also capable of oxidizing protein- and peptide-bound tyrosyl residues, resulting in the formation of inter- and intra-molecular crosslinks. Tyrosinases from apple (AT), potato (PT), the white rot fungus Pycnoporus sanguineus (PsT), the filamentous fungus Trichoderma reesei (TrT) and the edible mushroom Agaricus bisporus (AbT)...
Tipo: Journal Article Palavras-chave: ENZYME; SPECTROSCOPIE; ACTIVITE ENZYMATIQUE; INHIBITION ENZYMATIQUE PYCNOPORUS SANGUINEUS; TRICHODERMA REESI.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c0091e56&uri=/notices/prodinra1/2008/06/
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Construction of engineered bifunctional enzymes and their overproduction in Aspergillus niger for improved enzymatic tools to degrade agricultural by-products Inra
Levasseur, A.; Navarro, D.; Punt, P.J.; Belaïch, J.P.; Asther, M.; Record, E..
Two chimeric enzymes, FLX and FLXLC, were designed and successfully overproduced in Aspergillus niger. FLX construct is composed of the sequences encoding the feruloyl esterase A (FAEA) fused to the endoxylanase B (XYNB) of A. niger. A C-terminal carbohydrate-binding module (CBM family 1) was grafted to FLX, generating the second hybrid enzyme, FLXLC. Between each partner, a hyperglycosylated linker was included to stabilize the constructs. Hybrid proteins were purified to homogeneity, and molecular masses were estimated to be 72 and 97 kDa for FLX and FLXLC, respectively. Integrity of hybrid enzymes was checked by immunodetection that showed a single form by using antibodies raised against FAEA and polyhistidine tag. Physicochemical properties of each...
Tipo: Journal Article Palavras-chave: SON DE CEREALE; ACTIVITE ENZYMATIQUE ASPERGILLUS NIGER.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20086808f0a6&uri=/notices/prodinra1/2008/08/
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FOLy: An integrated database for the classification and functional annotation of fungal oxidoreductases potentially involved in the degradation of lignin and related aromatic compounds Inra
Levasseur, A.; Piumi, F.; Coutinho, P.M.; Rancurel, C.; Asther, M.; Delattre, M.; Henrissat, B.; Pontarotti, P.; Asther, M.; Record, E..
The breakdown of lignin by fungi is a key step during carbon recycling in terrestrial ecosystems. This process is of great interest for green and white biotechnological applications. Given the importance of these enzymatic processes, we have classified the enzymes potentially involved in lignin catabolism into sequence-based families and integrated them in a newly developed database, designated Fungal Oxidative Lignin enzymes (FOLy). Families were defined after sequence similarity searches starting from protein sequences and validated by the convergence of results with biochemical experiments reported in the literature. The resulting database was applied as a tool for the functional annotation of genomes from different fungi, namely (i) the Basidiomycota...
Tipo: Journal Article Palavras-chave: COMPOSE AROMATIQUE; BASIDIOMYCETES; BASE DE DONNEES; ASCOMYCETES MELANIN SYNTHESIS; FUNCTIONAL CLASSIFICATION; LIGNIN DEGRADATION; FUNGAL GENOME; FUNCTIONAL ANNOTATION; DATABASE; GREEN BIOTECHNOLOGY.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20087e29edda&uri=/notices/prodinra1/2008/06/
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Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application Inra
Record, E.; Asther, M.; Sigoillot, J.C.; Pagès, S.; Punt, P.J.; Delattre, M.; Haon, M.; Van den Hondel, C.A.M.J.J.; Sigoillot, J.C.; Lesage-Meessen, L.; Asther, M..
A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l(-1)), improved FAEA activity 24.5-fold and a yield of 1 g l(-1) of the corresponding protein in the culture medium was achieved. The secreted FAEA was purified 3.5-fold to homogeneity in a two-step purification procedure with a recovery of 69%. The overproduced protein was characterised and presented properties in good agreement with those of native FAEA. The recombinant FAEA was tested for wheat straw pulp...
Tipo: Journal Article Palavras-chave: BLANCHIMENT FERULOYL ESTERASE; WHEAT-STRAW PULP; ENZYMATIC TREATMENT; LACCASE; XYLANASE.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20071bf46680&uri=/notices/prodinra1/2010/09/
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Oxidative degradation of model lipids representative for main paper pulp lipophilic extractives by the laccase-mediator system Inra
Molina, S.; Rencoret, J.; del Rio, J.C.; Lomascolo, A.; Record, E.; Martinez, A.T.; Gutierrez, A..
Different model lipids—alkanes, fatty alcohols,fatty acids, resin acids, free sterols, sterol esters, andtriglycerides—were treated with Pycnoporus cinnabarinuslaccase in the presence of 1-hydroxybenzotriazole asmediator, and the products were analyzed by gas chromatography.The laccase alone decreased the concentration ofsome unsaturated lipids. However, the most extensive lipidmodification was obtained with the laccase–mediatorsystem. Unsaturated lipids were largely oxidized and thedominant products detected were epoxy and hydroxy fattyacids from fatty acids and free and esterified 7-ketosterolsand steroid ketones from sterols and sterol esters. Theformer compounds suggested unsaturated lipid attack viathe corresponding hydroperoxides. The enzymatic...
Tipo: Journal Article Palavras-chave: ACTIVITE ENZYMATIQUE OXIDATIVE ENZYME; GAS CHROMATOGRAPHY; LACCASE-MEDIATOR SYSTEM; FUNGAL ENZYME; MODEL LIPIDS; REACTION MECHANISMS; PAPER PULP; PITCH DEPOSITS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c803f667&uri=/notices/prodinra1/2010/09/
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Transgenic rice as a novel production system for Melanocarpus and Pycnoporus laccases Inra
de Wilde, C.; Uzan, E.; Zhou, Z.; Kruus, K.; Andberg, M.; Buchert, J.; Record, E.; Asther, M.; Lomascolo, A..
Laccases have numerous biotechnological applications, among them food processing. The widespread use of laccases has increased the demand for an inexpensive and safe source of recombinant enzyme. We explored the use of a rice-based system for the production of two fungal laccases derived from the ascomycete Melanocarpus albomyces and the basidiomycete Pycnoporus cinnabarinus. High-expression levels of active recombinant laccases were achieved by targeting expression to the endosperm of rice seeds. The laccase cDNAs were fused to a plant-derived signal sequence for targeting to the secretory pathway, and placed under the control of a constitutive seed-specific promoter fused to an intron for enhanced expression. This construct enabled the recovery of on...
Tipo: Journal Article Palavras-chave: PLANTE TRANSGENIQUE; BIOTECHNOLOGIE INDUSTRIELLE; PROPRIETE BIOCHIMIQUE; ASCOMYCETE; BASIDIOMYCETE LACCASE; RECOMBINANT FUNGAL ENZYME; RICE; HETEROLOGOUS PROTEIN; TRANSGENIC PLANT; BIOCHEMICAL PROPERTIES.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c973f6c6&uri=/notices/prodinra1/2008/10/
Registros recuperados: 7
Primeira ... 1 ... Última
 

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