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	Provedor de dados 58 (2) Autor Rodrigues,Maria Filomena de Andrade (2) Castillo,Boris Gutarra (1) Fernández,Rubén Cuervo (1) Maiorano,Alfredo Eduardo (1) Maresma,Beatriz Guilarte (1) Matias,Fernanda (1) Silva,Elda Sabino da (1) Mais... Palavra-chave Aspergillus oryzae (1) Burkholderia (1) Burkholderia cepacia complex (1) Fructooligosaccharides (1) Fructosyltransferase (1) Genomovar-type (1) Mcl-PHA (1) Mutagenesis (1) Polyhydroxyalkanoates (1) Polyhydroxybutyrate (1) UV irradiation (1) Β-fructofuranosidase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase 58 Maresma,Beatriz Guilarte; Castillo,Boris Gutarra; Fernández,Rubén Cuervo; Silva,Elda Sabino da; Maiorano,Alfredo Eduardo; Rodrigues,Maria Filomena de Andrade. Aspergillus oryzae IPT-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using UV irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. Strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in Petri dish as the enzyme source. Seven mutants with fructofuranosidase activity values relative to the parent culture between 140 -190% were selected from survivors grown at temperature of 40ºC or 0.018% (w/v) sodium dodecyl sulfate concentration. They were cultivated on a rotary shaker to characterize mycelium and extracellular fructosyltransferase activities. Three mutants named IPT-745, IPT-746 and IPT-748... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus oryzae; Mutagenesis; Β-fructofuranosidase; Fructosyltransferase; Fructooligosaccharides; UV irradiation. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100027 New PHA products using unrelated carbon sources 58 Matias,Fernanda; Rodrigues,Maria Filomena de Andrade. Polyhydroxyalkanoates (PHA) are natural polyesters stored by a wide range of bacteria as carbon source reserve. Due to its chemical characteristics and biodegradability PHA can be used in chemical, medical and pharmaceutical industry for many human purposes. Over the past years, few Burkholderia species have become known for production of PHA. Aside from that, these bacteria seem to be interesting for discovering new PHA compositions which is important to different industrial applications. In this paper, we introduce two new strains which belong either to Burkholderia cepacia complex (Bcc) or genomovar-type, Burkholderia cepacia SA3J and Burkholderia contaminans I29B, both PHA producers from unrelated carbon sources. The classification was based on 16S... Tipo: Info:eu-repo/semantics/article Palavras-chave: Burkholderia; Burkholderia cepacia complex; Genomovar-type; Mcl-PHA; Polyhydroxyalkanoates; Polyhydroxybutyrate. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400017 Registros recuperados: 2 Primeira ... 1 ... Última

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