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Evaluation of DNA extraction protocols for Brucella abortus pcr detection in aborted fetuses or calves born from cows experimentally infected with strain 2308 BJM
Matrone,M.; Keid,L.B.; Rocha,V.C.M.; Vejarano,M.P.; Ikuta,C.Y.; Rodriguez,C.A.R.; Ferreira,F.; Dias,R.A.; Ferreira Neto,J.S.
The objective of the present study was to improve the detection of B. abortus by PCR in organs of aborted fetuses from infected cows, an important mechanism to find infected herds on the eradication phase of the program. So, different DNA extraction protocols were compared, focusing the PCR detection of B. abortus in clinical samples collected from aborted fetuses or calves born from cows challenged with the 2308 B. abortus strain. Therefore, two gold standard groups were built based on classical bacteriology, formed from: 32 lungs (17 positives), 26 spleens (11 positives), 23 livers (8 positives) and 22 bronchial lymph nodes (7 positives). All samples were submitted to three DNA extraction protocols, followed by the same amplification process with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Brucellosis; Abortion; PCR; DNA extraction.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300010
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Isolation of rotavirus from asymptomatic dogs in Brazil Arq. Bras. Med. Vet. Zootec.
Ruiz,V.L.A.; Brandão,P.E.; Gregori,F.; Rodriguez,C.A.R.; Souza,S.L.P.; Jerez,J.A..
Tipo: Info:eu-repo/semantics/article
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000400031
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Tuberculosis determined by Mycobacterium bovis in captive waterbucks (Kobus ellipsiprymnus) in São Paulo, Brazil BJM
Rocha,V.C.M.; Corrêa,S.H.R.; Oliveira,E.M.D.; Rodriguez,C.A.R.; Fedullo,J.D.; Matrone,M.; Setzer,A.; Ikuta,C.Y.; Vejarano,M.P.; Figueiredo,S.M.; Ferreira Neto,J.S..
Two waterbucks from São Paulo Zoo Foundation exhibited respiratory symptoms in July 2004. After euthanasia, granulommas in lungs and mediastinic lymph nodes were observed. Acid-fast bacilli isolated were identified as Mycobacterium bovis spoligotype SB0121 by PRA and spoligotyping. They were born and kept in the same enclosure with the same group, without any contact to other species housed in the zoo. This is the first detailed description of M. bovis infection in Kobus ellipsiprymnus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Mycobacterium bovis; Kobus ellipsiprymnus; Waterbuck; Zoo.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200040
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