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Lourenço,TC; Mendonça,EP; Nalevaiko,PC; Melo,RT; Silva,PL; Rossi,DA. |
The aim of this study was to evaluate the efficacy of turmeric (Curcuma longa), also known in Brazil as saffron, on the reduction of Staphylococcus aureus and Escherichia coli counts in chicken meat. Forty breast meat samples were divided in two groups (A and B). In group A, 10³-10(4)E. coli (ATCC 25922) cells were inoculated and group B samples were inoculated with 10(4)-10(5)S. aureus (ATCC 9801) cells, after which each group was divided in three samples. The first sample was analyzed immediately after inoculation. The second sample (control group) was stored at 4 ºC for 48 hours and turmeric at 1% (w/w) was added to the third sample, which was homogenized and then stored under the same conditions as the second sample. E. coli and S. aureus were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Turmeric; Bacterial growth; Escherichia coli; Staphylococcus aureus. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000200002 |
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Fonseca,BB; Ferreira Júnior,A; Santos,JP dos; Coelho,LR; Rossi,DA; Melo,RT; Mendonça,EP; Araújo,TG; Alves,RN; Beletti,ME. |
Abstract Campylobacteriosis is a worldwide foodborne zoonosis disease caused by Campylobacter jejuni. This microorganism is considered a commensal bacterium in chicken hosts. C. jejuni produces epithelial cell modifications and induces a cytokine gene transcription innate immunity repertoire. In the present study, we describe the invasiveness, morphological cellular modifications, and transcript level expressions of innate immune cytokines from C. jejuni-inoculated chicken ileum explants. C. jejuni was internalized by epithelial ileum cells at 15 minutes postinoculation (p.i.) and was detected intracellularly for 4hs (p.i.). Inoculated explants displayed significant increases in cell height. C. jejuni induced a significant elevation of Transforming Growth... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; Chicken; Explants; TGF-b3; IL-1b. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100063 |
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Fonseca,BB; Soncini,RA; Vieira,FL; Siqueira,MS; Guimarães,AR; Beletti,ME; Rossi,DA. |
Campylobacter sp is an important agent that causes foodborne infection, particularly in food of poultry origin. Therefore, the efficient control of the transmission routes in chicken farms is of outmost importance to prevent it from spreading. In chicken farms, the main transmission route of this microorganism is horizontal, as the vertical route continues to be the object of inconclusive researches. The objective of this study was to verify the presence of Campylobacter sp in breeder hens, meconium, and other organs of day-old chicks derived from these breeders in order to obtain information on vertical transmission. Microbiological analyses were performed, using cloacal swabs from 279 breeder hens. Positive breeders were segregated, and the presence of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter sp; Chickens; Vertical transmission; Epidemiology. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400010 |
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Raghiante,F; Rocha,TS; Rossi,DA; Silva,PL. |
This study aimed at determining the minimum time required for the penetration of Salmonella Heidelberg inside the eggs after contact with contaminated material. Recently-collected brown and white eggs from laying hens between 45-50 weeks of age, reared in a commercial poultry house, were artificially contaminated by contact with wood shavings moistened with liquid inoculum of Salmonella Heidelberg in stationary-growth phase (10³-10(4) CFU g-1). According to type (white or brown), eggs were distributed into three different groups, with four replicates each: negative control group (no artificial contamination), positive control group (analyzed externally immediately after contamination and internally after the maximum storage period of the test group) and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eggs; Penetration time; Salmonella Heidelberg. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000400009 |
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Ferreira,BC; Couto,RM; Ecco,R; Coelho,HE; Rossi,DA; Beletti,ME; Silva,PL. |
ABSTRACT This study aimed at proposing a new technical criteria for condemnation of turkey carcasses due to fowlpox in turkeys as a contribution for the work of the Brazilian Federal Meat Inspection Service. Skin samples from 30 carcasses of a flock of 840 turkeys (Meleagris gallopavo), previously vaccinated for fowlpox and slaughtered in June 2013, were collected. Samples were submitted to histological examination under light microscopy. The virus was identified using standard PCR techniques. The main histological findings were hyperplasia and hydropic degeneration of the epithelium and the presence of intracytoplasmic eosinophilic inclusion bodies. PCR results yielded 83.3% positive and 16.7% negative samples. Fowlpox virus is species specific, and there... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Condemnations; Fowlpox; Processing plant; SIF. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200225 |
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Coelho,LR; Melo,RT; Monteiro,GP; Reischak,D; Mendonça,AO; Tavares,AAS; Rossi,DA. |
ABSTRACT The analysis of Salmonella in the feces and the birds’ environment is a way of monitoring the colonization in the flocks and verifying the need for the introduction of stricter controls, in such a way that the results of the tests should be known before being sent for slaughter. The polymerase chain reaction (PCR), as well as other rapid methods represent alternatives increasingly used to detect enteric pathogens, but they need proof of effectiveness for their wide use. The aim of this study was to evaluate the equivalence between the results obtained by the methods: real-time PCR (BAX® System), Modified Rappaport-Vassiliadis Semi-solid Medium (MSRV) (ISO 6579) and the traditional method of official reference in Brazil for research of S.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BAX® System; MSRV; Salmonella Enteritidis; S. Typhimurium; Molecular biology. |
Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200303 |
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