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Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran Scientia Agricola
Golombieski,Jaqueline Ineu; Marchesan,Enio; Camargo,Edinalvo Rabaioli; Salbego,Joseânia; Baumart,Joele Schmitt; Loro,Vania Lucia; Machado,Sérgio Luiz de Oliveira; Zanella,Renato; Baldisserotto,Bernardo.
Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758), grass carp (Ctenopharyngodon idella, Valenciennes, 1844), and bighead carp (Aristichthys nobilis, Richardson, 1845) were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50) of diafuran and the acetylcholinesterase (AChE) activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term): common...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyprinus carpio; Ctenopharyngodon idella; Aristichthys nobilis; Pesticide; Lethal concentration.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000400003
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Anesthesia and sedation of map treefrog (Hypsiboas geographicus) tadpoles with essential oils Ciência Rural
Salbego,Joseânia; Maia,Janna Laely dos Santos; Toni,Cândida; Rodrigues,Amanda Sousa Silva; Sousa,Elen Monique Oliveira; Silva,Lenise Vargas Flores da; Mourão,Rosa Helena Veras; Barata,Lauro Euclides Soares; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
ABSTRACT: The goal of this study was to investigate the sedative and anesthetic properties of essential oils (EOs) in map treefrog tadpoles (Hypsiboas geographicus) and to determine the sedation and deep anesthesia induction times as well as the recovery time. The tadpoles were exposed to one of the EOs from three plant species: Aniba rosaeodora (EOAR - 25, 50, 100 or 200µL L-1), Lippia origanoides (EOLO - 13, 25, 50, 100 or 200µL L-1), and Lippia alba (either chemotype citral [EOL-C - 25, 50, 100 or 200µL L-1] or linalool [EOL-L - 50, 75, 100 or 200µL L-1]) (n = 8 per replicate). The tadpoles exposed to 25 and 50µL L-1 EOL-C and EOL-L, respectively, were not anesthetized within 30min (the maximum time of observation), and those exposed to 200µL L-1 EOLO...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amphibians; Animal welfare; Natural anesthetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100403
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Different feeding habits influence the activity of digestive enzymes in freshwater fish Ciência Rural
Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia.
ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine. Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406
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Essential oils from Citrus x aurantium and Citrus x latifolia (Rutaceae) have anesthetic activity and are effective in reducing ion loss in silver catfish ( Rhamdia quelen ) Neotropical Ichthyology
Lopes,Jane Mello; Souza,Carine de Freitas; Schindler,Bianca; Pinheiro,Carlos Garrido; Salbego,Joseânia; Siqueira,Jefferson Costa de; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
ABSTRACT This study investigated the anesthetic effect of the essential oils (EOs) from the peel of Citrus x aurantium (EOCA) and Citrus x latifolia (EOCL) on silver catfish Rhamdia quelen. Fish were exposed to different concentrations of EOCA and EOCL to determine time of anesthesia induction and recovery. Induction of anesthesia was observed in all fish exposed to 400, 600 or 800 μL L−1 EOCA and 300, 400 or 500 μL L−1 EOCL. Another group of fish were exposed for 8 h to 50, 100, or 200 μL L−1 of either EOs. Overall, fish exposed to ethanol and both EOs presented higher ventilatory frequencies (VF) than the control group throughout the 8 h of exposure. Net ion (Na+, K+ and Cl−) effluxes and ammonia excretion were significantly lower in fish exposed to 50,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Ion fluxes; Lemon oil; Orange oil; Ventilatory frequency.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000200204
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Essential oils of Cunila galioides and Origanum majorana as anesthetics for Rhamdia quelen: efficacy and effects on ventilation and ionoregulation Neotropical Ichthyology
Cunha,Jessyka A. da; Scheeren,Cecília Á.; Salbego,Joseânia; Gressler,Luciane T.; Madaloz,Laurício M.; Bandeira-Junior,Guerino; Bianchini,Adriane E.; Pinheiro,Carlos G.; Bordignon,Sérgio A. L.; Heinzmann,Berta M.; Baldisserotto,Bernardo.
ABSTRACT This study evaluated anesthetic efficacy and possible effects of the essential oils (EOs) of Cunila galioides (EOC) and Origanum majorana (EOO) on ventilatory rate (VR) and ionoregulation in Rhamdia quelen. In the anesthesia assessments, 50, 100, 200 and 300 μL L-1 EOC and 50, 100, 200, 300, 400 and 500 μL L-1 EOO were tested, and time for induction to sedation and anesthesia stages, as well as recovery, were taken. A second trial employed lower concentrations of both EOs, 10, 25, 50 and 100 μL L-1, in order to verify VR and Na+, K+ and Cl- whole body net fluxes. Sedation was achieved with both oils at 100 µL L-1, and anesthesia at ≥ 200 µL L-1. There was no significant difference between control and EO-treated groups regarding VR, but all fish...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ionoregulatory balance; Marjoram; Pennyroyal; Silver catfish; Ventilatory rate.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100213
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Glyphosate on digestive enzymes activity in piava (Leporinus obtusidens) Ciência Rural
Salbego,Joseânia; Pretto,Alexandra; Silva,Vera Maria Machado da; Loro,Vania Lucia; Lazzari,Rafael; Gioda,Carolina Rosa; Baldisserotto,Bernardo.
The effects of glyphosate, a nonselective herbicide (1.0 or 5.0mg L-1) on digestive enzymes activity (stomach and intestine) were evaluated in juveniles of piava (Leporinus obtusidens) after 90 days of exposure. The activity of acid protease, trypsin, chymotrypsin and amylase increased with the increase of glyphosate concentration. These results indicate that glyphosate affects digestive enzyme activities in this species, and may be an indicator of poor nutrient availability when fish survive in herbicide-contaminated water.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Herbicide; Acid protease; Amylase; Trypsin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000901603
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Physiological responses of Rhamdia quelen (Siluriformes: Heptapteridae) to anesthesia with essential oils from two different chemotypes of Lippia alba Neotropical Ichthyology
Souza,Carine de Freitas; Baldissera,Matheus Dellaméa; Salbego,Joseânia; Lopes,Jane Mello; Vaucher,Rodrigo de Almeida; Mourão,Rosa Helena Veras; Caron,Braulio O.; Heinzmann,Berta Maria; Silva,Lenise Vargas Flôres da; Baldisserotto,Bernardo.
ABSTRACT The aim of this study was to evaluate if Lippia alba has different chemotypes according to the chemical composition of the essential oil (EO) considering collection site, and if the EO may have different effects on blood and plasma parameters in silver catfish, Rhamdia quelen, during and immediately after anesthesia. The citral (EO-C) and linalool (EO-L) chemotypes were identified, and both presented similar anesthetic effects for silver catfish. Fish were exposed to two concentrations of each EO, which induced slow and fast anesthesia (100 and 300 µL L-1, respectively). Blood ions did not change at any time of anesthesia induction and recovery and, therefore, the electrolyte balance was not altered. Blood gases oscillated through all exposure and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood gas; Cortisol; Glucose; Plasma ions; Silver Catfish.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100203
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Rhamdia quelen (Quoy & Gaimard, 1824), submitted to a stressful condition: effect of dietary addition of the essential oil of Lippia alba on metabolism, osmoregulation and endocrinology Neotropical Ichthyology
Souza,Carine de Freitas; Salbego,Joseânia; Gressler,Luciane T.; Golombieski,Jaqueline I.; Ferst,Juliana G.; Cunha,Mauro A.; Heinzmann,Berta M.; Caron,Braulio O.; Glanzner,Werner G.; Gonçalves,Paulo B. D.; Baldisserotto,Bernardo.
ABSTRACT The aim of this study was to evaluate the effect of the essential oil of Lippia alba (EOLA) as a feed additive on ionoregulatory and metabolic parameters and pituitary hormones expression in silver catfish, Rhamdia quelen , submitted to a stressful condition (stocking density of 10.6 kg m-3 and limited space). Fish were fed with different concentrations of EOLA (0.0 - control, 0.25 and 0.50 mL kg food-1) for 20 days. Metabolic parameters were not affected by the diet, with the exception of alanine aminotransferase, which was higher in the liver of fish fed 0.50 mL EOLA kg food-1. Plasma ions and activity of H+-ATPase did not change, but fish fed 0.25 mL EOLA kg food-1 presented higher Na+/K+-ATPase activity. Somatolactin expression in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cortisol; Enzymatic activity; Growth hormone; Somatolactin.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000400707
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The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen) after transportation Neotropical Ichthyology
Salbego,Joseânia; Becker,Alexssandro G.; Gonçalves,Jamile F.; Menezes,Charlene C.; Heldwein,Clarissa G.; Spanevello,Rosélia M.; Loro,Vania L.; Schetinger,Maria Rosa C.; Morsch,Vera M.; Heinzmann,Berta M.; Baldisserotto,Bernardo.
This study investigated the effects of the essential oil (EO) from Lippia alba on biochemical parameters related to oxidative stress in the brain and liver of silver catfish (Rhamdia quelen) after six hours of transport. Fish were transported in plastic bags and divided into three treatments groups: control, 30 µL L- 1 EO from L.alba and 40 µL L-1 EO from L.alba. Prior to transport, the fish were treated with the EO from L. alba (200 µL L -1 for three minutes), except for the control group. Fish transported in bags containing the EO did not have any alterations in acetylcholinesterase, ecto -nucleoside triphosphate diphosphohydrolase and 5'nucleotidase activity in the brain or superoxide dismutase activity in the liver. The hepatic catalase (CAT),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthetics; Antioxidant defenses; Biochemical parameters; Fish transport; Oxidative stress.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400811
Registros recuperados: 9
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