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Lima bean (Phaseolus lunatus) seed coat phaseolin is detrimental to the cowpea weevil (Callosobruchus maculatus) BJMBR
Moraes,R.A.; Sales,M.P.; Pinto,M.S.P.; Silva,L.B.; Oliveira,A.E.A.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J..
The presence of phaseolin (a vicilin-like 7S storage globulin) peptides in the seed coat of the legume Phaseolus lunatus L. (lima bean) was demonstrated by N-terminal amino acid sequencing. Utilizing an artificial seed system assay we showed that phaseolin, isolated from both cotyledon and testa tissues of P. lunatus, is detrimental to the nonhost bruchid Callosobruchus maculatus (F) (cowpea weevil) with ED50 of 1.7 and 3.5%, respectively. The level of phaseolin in the seed coat (16.7%) was found to be sufficient to deter larval development of this bruchid. The expression of a C. maculatus-detrimental protein in the testa of nonhost seeds suggests that the protein may have played a significant role in the evolutionary adaptation of bruchids to legume seeds.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lima bean; Phaseolin (vicilin); 7S storage globulin; Seed coat; Cowpea weevil; Resistance to bruchids.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200005
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Vicilins (7S storage globulins) of cowpea (Vigna unguiculata) seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae) larvae BJMBR
Sales,M.P.; Pimenta,P.P.; Paes,N.S.; Grossi-de-Sá,M.F.; Xavier-Filho,J..
The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata) seed vicilins (7S storage proteins) bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bruchid beetles; Callosobruchus maculatus; Cowpea seeds; Vigna unguiculata; Vicilins (7S storage proteins); Immunolocalization; Midgut.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100003
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