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Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. Biological Sciences
Santana, Gleuvânia Marques; Sousa, Josefa Janaína do Anjos; Peron, Ana Paula.
This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p < 0.05). From the results, we observed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tecnolologia de alimentos citogenética azo dye; Cell division; Cellular aberrations; Cytotoxicity.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119
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Toxicity in food flavorings at the cellular level associated with each other at different doses Biological Sciences
Silva, Sara Iolanda Oliveira da; Santana, Gleuvânia Marques; Sales, Ila Monize Sousa; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
This study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in combination, in which one of the three doses of a flavoring was combined with a different dose of one of the two other flavor additives studied. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and then analyzed, under light microscopy, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. All the treatments with combinations between the flavorings Chocolate/Strawberry and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aroma and flavor additives; Cytotoxicity; Genotoxicity A. cepa Citogenética.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475
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