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Santos,Leonardo Silva; Soriano,Maria del Pilar Camarantin; Mirabal-Gallardo,Yaneris; Carrasco-Sanchez,Veronica; Nachtigall,Fabiane Manke; Pereira,Iris; Pereira,Eugenia. |
The aim of this work was to study a fast, new, sensitive, and simple method for the chemotaxonomic classification of Chilean lichens (Teloschistes chrysophthalmus, Ramalina farinacea, Usnea pusilla, Ramalina chilensis and Stereocaulon ramulosum) using MALDI-TOF-MS and UPLC-ESI(-)-MS data. Lichens soluble proteins fingerprints were acquired by MALDI-TOF-MS and they were analyzed by chemometric (PCA). Lichens organic extracts fingerprints were obtained by UPLC-ESI(-)-MS. MALDI-TOF-MS associated with chemometric analysis was used to detect new m/z patterns of soluble proteins that were compared with Protein Data Bank of UnitPro. These data also permitted the satisfactory distinction among the families and species. UPLC-ESI(-)-MS fingerprints analyses of the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chilean lichens; Chemotaxonomic; Fingerprint; MALDI-TOF-MS; UPLC-ESI(-)-MS. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200244 |
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