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Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina BJM
Lucero Estrada,Cecilia S.M.; Alcaráz,Lucia E.; Satorres,Sara E.; Manfredi,Eduardo; Velázquez,Lidia del C..
An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Enterotoxigenic strains; Fruit salads; Antimicrobial compounds; Multiplex PCR.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400018
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Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens BJM
Alcaráz,Lucía E.; Satorres,Sara E.; Lucero,Rodolfo M.; Centorbi,Olga N. Puig de.
Ninety-two coagulase negative staphylococci (CNS) (forty-five of clinical origin and forty-seven of environmental origin), collected in a hospital in San Luis, Argentina, from March to June, 1999, were identified to species level by the ID 32 Staph and API Staph System (bioMérieux). Slime production was investigated by the quantitative and qualitative methods. Oxacillin susceptibility was determined by the disk diffusion test (1 µg), the agar dilution method (0.125 to 4 mg/ml) and agar screen (6 µg/ml). The presence of mecA gene was investigated by PCR. The clinical CNS species most commonly isolated were S. epidermidis, S. haemolyticus, S. hominis and S. saprophyticus. The frequency of slime production by clinical and environmental isolates was similar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coagulase-negative staphylococci; Slime; MecA; Oxacillin-R.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100010
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