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Caracterization of Aujeszky's disease virus isolated from South Brazil in the last twenty years by restriction enzyme analysis BJM
Schaefer,Rejane; Zanella,Janice Ciacci; Mores,Nelson; Pan,Kleitton Adolfo; Dambros,Régia Maria Feltrim; Schiochet,Marisete Fracasso; Coldebella,Michele.
Aujeszky's disease virus (ADV) belongs to Herpesviridae family and is an important etiological agent which infects pigs causing economic losses in swine producing countries worldwide and international trade restrictions to products of swine origin. An eradication program for ADV was established in Santa Catarina State since 2001. The last outbreak was reported in July 2004 and since then none has been reported. The disease has been controlled with the use of a genetic modified vaccine and elimination of seropositives. Aiming the characterization of ADV isolated in the South of Brazil in the last twenty years (1983-2003), a retrospective study based on the genomic analysis of the isolates through a digestion of viral genomic DNA with restriction enzyme Bam...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky's disease virus; Swine; Restriction endonuclease analysis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300035
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Cloning and expression of Aujeszky's disease virus glycoprotein E (gE) in a baculovirus system BJM
Dambros,Régia Maria Feltrin; Ribeiro,Bergman Moraes; Aguiar,Raimundo Wagner de S.; Schaefer,Rejane; Esteves,Paulo Augusto; Perecmanis,Simone; Simon,Neide Lisiane; Silva,Nayara Cavalcante; Coldebella,Michele; Ciacci-Zanella,Janice Reis.
Aujeszky' s disease (AD) is an infectious disease causing important economic losses to the swine industry worldwide. The disease is caused by an alpha-herpesvirus, Aujeszky' s disease virus (ADV), an enveloped virus with a double stranded linear DNA genome. The ADV genome encodes 11 glycoproteins, which are major targets for the immune system of the host in response to the infection. The glycoprotein E (gE) is a non-essential protein and deletion of the gE gene has been used for the production of marker vaccines. Aiming to develop molecular reagents for the production of a gE specific ELISA test, the gE gene was amplified by PCR, cloned and expressed into a baculovirus expression system. The recombinant DNA vector pFastBac-gE-ADV was used for site-specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky; Glycoprotein E; Baculovírus; Recombinant.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300021
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Structure analysis of capsid protein of Porcine circovirus type 2 from pigs with systemic disease BJM
Gava,Danielle; Serrão,Vitor Hugo Balasco; Fernandes,Lana Teixeira; Cantão,Mauricio Egídio; Ciacci-Zanella,Janice Reis; Morés,Nelson; Schaefer,Rejane.
Abstract Economic losses with high mortality rate associated with Porcine circovirus type 2 (PCV2) is reported worldwide. PCV2 commercial vaccine was introduced in 2006 in U.S. and in 2008 in Brazil. Although PCV2 vaccines have been widely used, cases of PCV2 systemic disease have been reported in the last years. Eleven nursery or fattening pigs suffering from PCV2 systemic disease were selected from eight PCV2-vaccinated farms with historical records of PCV2 systemic disease in Southern Brazil. PCV2 genomes were amplified and sequenced from lymph node samples of selected pigs. The comparison among the ORF2 amino acid sequences of PCV2 isolates revealed three amino acid substitutions in the positions F57I, N178S and A190T, respectively. Using molecular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Porcine circovirus type 2 (PCV2); Capsid protein; Molecular modeling and antigenic epitope.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200351
Registros recuperados: 3
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