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Registros recuperados: 8
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A new methodology for the improvement of diagnostic immunohistochemistry in canine veterinary pathology: automated system using human monoclonal and polyclonal antibodies Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Silva,P.; Rêma,A.; Gärtner,F.; Gobbi,H.; Tafuri,W.L.; Schmitt,F.C..
The authors describe their experience with an automated immunohistochemical system applied to canine tissue samples. Twenty human cellular markers specific monoclonal and polyclonal antibodies and two different antigen retrieval methods were used in normal and neoplastic breast tissue, as well as skin samples obtained from female dogs of pure and mixed breeds. The antibodies tested were the most frequently used in human and veterinary medicine studies, employed with diagnostic purposes in breast pathology, as well as in cancer research. Most of them may be used to study other normal and abnormal tissues and included cytokeratins, progesterone receptor, c-erbB2, p53, MIB-1, PCNA, EMA, vimentin, desmin, alpha-actin, S-100, pan-cadherin, and E-cadherin. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Immunohistochemistry; Automated system; Mammary gland.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000300010
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Assessment of cell proliferation and prognostic factors in canine mammary gland tumors Arq. Bras. Med. Vet. Zootec.
Dutra,A.P.; Azevedo Júnior,G.M.; Schmitt,F.C.; Cassali,G.D..
Three methods for the analysis of cell proliferation, mitotic index/10 high-power fields (10 HPF), mitotic index/four sets of 10 HPF (40 HPF), and MIB-1 index were evaluated in a series of canine mammary gland tumors, as well as the possible correlation between them. Fifty-six canine mammary gland tumors, including 23 benign and 33 malignant, were studied. In addition, the prognostic impact of mitotic index/10 HPF, and histological malignancy grade were evaluated in 17 malignant tumors, being seven ductal and 10 metaplastic carcinomas. The three methods used to evaluate cell proliferation were correlated with the prognostic impact of mitotic index/10 HPF and histological malignancy grade. The results showed a strong association between mitotic figure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine mammary tumors; MIB-1 index; Mitosis counting.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600015
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c-erbB-2 expression and nuclear pleomorphism in canine mammary tumors BJMBR
Dutra,A.P.; Granja,N.V.M.; Schmitt,F.C.; Cassali,G.D..
The objective of the present investigation was to study the expression of c-erbB-2 and MIB-1 and try to associate them with morphological features of the cell such as nuclear pleomorphism, mitotic count and histological grade in a series of 70 canine mammary gland tumors, 22 of them benign and 48 malignant. Tumors were collected at the Veterinary Hospital of UFMG (Brazil) and the Veterinary Faculty of Porto University (Portugal). c-erbB-2 expression was determined according to the guidelines provided by the manufacturer of the HercepTest system and nuclear pleomorphism, mitotic count and histological grade according the Elston and Ellis grading system. The HercepTest is the FDA-approved in vitro diagnostic test marketed by Dako. It is a semi-quantitative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine mammary tumors; Nuclear pleomorphism; Breast cancer; C-erbB-2 expression; MIB-1 expression.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100013
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Cytological diagnosis of a metastatic canine mammary tumor in pleural effusion Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Gärtner,F.; Vieira da Silva,M.J.; Schmitt,F.C..
Descrevem-se os achados citomorfológicos de um tumor maligno de mama em uma cadela Poodle de sete anos de idade, o qual foi observado inicialmente pelo exame citológico do derrame pleural. Comparam-se os aspectos citológicos do derrame pleural e punção aspirativa com agulha fina do tumor com aqueles descritos para o câncer de mama na espécie humana.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dog; Cytology; Pleural effusion; Mammary tumor.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400003
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DNA flow cytometry of canine mammary tumors: comparative aspects with human breast tumors Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Salvador,A.; Freitas,C.; Dutra,A.P.; Schmitt,F.C..
Flow cytometric analysis of DNA content was performed on 28 samples of canine mammary tumors. Nine of them were benign and 19 were malignant. All benign tumors and 11 malignant tumors (57.9%) were diploid (P<0.05). Form the aneuploid tumors, five (26.3%) were hyperdiploid, one (5.3%) hypodiploid, one (5.3%) near triploid and one (5.3%) multiploid. The analysis of the expression of the markers PR and CD31 revealed a significant difference between diploid and aneuploid tumors (P<0.05). The immunoreactivity of PR was higher in diploid tumors, while the immunoreactivity of CD31 was stronger in aneuploid tumors. No difference between the markers MIB-1, c-erbB2, p53 and Cyclin D1 was observed (P>0.05). Using the flow cytometry analysis and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Breast cancer; Neoplasm; DNA flow cytometry; Immunohistochemistry.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500011
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Invasive micropapillary carcinoma of the dog mammary gland: a case report Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Serakides,R.; Gärtner,F.; Schmitt,F.C..
This report describes the morphological and immunohistochemical findings of two cases of breast invasive micropapillary carcinoma occurring in dogs. Histologically, the tumors are characterized by the presence of numerous irregular cystic formations filled out with nests of epithelial cells that exhibit a micropapillary pattern. These morphological features are characteristic of invasive micropapillary carcinoma in woman, a breast tumor not previously described in dogs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Mammary carcinoma; Mammary gland; Invasive micropapillary carcinoma.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000400006
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Pleomorphic lobular carcinoma of the canine mammary gland: histopathologic and immunohistochemical features Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Gärtner,F.; Schmitt,F.C..
This report describes the morphological and immunohistochemical findings of a case of mammary gland pleomorphic lobular carcinoma occurring in the canine species. Histologically, it was characterized by the presence of tumor cells loosely dispersed in the stroma or arranged in a linear pattern showing relatively abundant eosinophilic cytoplasm with an eccentric nuclei. These morphological features, characteristic of pleomorphic mammary gland lobular carcinoma in woman, were not previously described in dogs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Mammary carcinoma; Mammary gland; Pleomorphic lobular carcinoma.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000600006
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Protocol for the anatomopathological examination of canine mammary tumors Arq. Bras. Med. Vet. Zootec.
Ferreira,E.; Bregunci,G.C.; Schmitt,F.C.; Cassali,G.D..
Foi elaborado um protocolo para exame anatomopatológico de tumores de mama em cães, constituído de três partes: requisição, exame clínico e laudo histopatológico. O exame clínico contém dados sobre a descrição macroscópica da lesão. O laudo histopatológico constituiu-se de campos para descrição microscópica pormenorizada das lesões e classificação da principal lesão observada. A elaboração do protocolo tem como objetivo estabelecer critérios para estudos e pesquisas sobre neoplasias mamárias em animais e auxiliar no diagnóstico e prognóstico de lesões mamárias.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neoplasia; Mammary gland; Histopathology; Protocol; Dog.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000100017
Registros recuperados: 8
Primeira ... 1 ... Última
 

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