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Biochemical characterization and cytotoxic effect of the skin secretion from the red-spotted Argentina frog Argenteohyla siemersi (Anura: Hylidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Fusco,Luciano S.; Cajade,Rodrigo; Piñeiro,Jose M.; Torres,Ana M.; Silva,Igor R. F. da; Hyslop,Stephen; Leiva,Laura C.; Pimenta,Daniel C.; Bustillo,Soledad.
Abstract Background: Argenteohyla siemersi (red-spotted Argentina frog) is a casque-headed tree frog species belonging to the Hylidae family. This species has a complex combination of anti-predator defense mechanisms that include a highly lethal skin secretion. However, biochemical composition and biological effects of this secretion have not yet been studied. Methods: The A. siemersi skin secretion samples were analyzed by mass spectrometry and chromatographic analysis (MALDI-TOF/MS, RP-HPLC and GC-MS). Proteins were also studied by SDS-PAGE. Among the biological activities evaluated, several enzymatic activities (hemolytic, phospholipase A2, clotting, proteolytic and amidolytic) were assessed. Furthermore, the cytotoxic activity (cytolysis and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Argenteohyla siemersi; Hylidae; Skin secretion; Tree frog; Cytotoxicity.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100306
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