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A new methodology for the improvement of diagnostic immunohistochemistry in canine veterinary pathology: automated system using human monoclonal and polyclonal antibodies Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Silva,P.; Rêma,A.; Gärtner,F.; Gobbi,H.; Tafuri,W.L.; Schmitt,F.C..
The authors describe their experience with an automated immunohistochemical system applied to canine tissue samples. Twenty human cellular markers specific monoclonal and polyclonal antibodies and two different antigen retrieval methods were used in normal and neoplastic breast tissue, as well as skin samples obtained from female dogs of pure and mixed breeds. The antibodies tested were the most frequently used in human and veterinary medicine studies, employed with diagnostic purposes in breast pathology, as well as in cancer research. Most of them may be used to study other normal and abnormal tissues and included cytokeratins, progesterone receptor, c-erbB2, p53, MIB-1, PCNA, EMA, vimentin, desmin, alpha-actin, S-100, pan-cadherin, and E-cadherin. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Immunohistochemistry; Automated system; Mammary gland.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000300010
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Acoustic radiation force impulse (ARFI) elastography of testicular disorders in dogs: preliminary results Arq. Bras. Med. Vet. Zootec.
Feliciano,M.A.R.; Maronezi,M.C.; Simões,A.P.R.; Maciel,G.S.; Pavan,L.; Gasser,B.; Silva,P.; Uscategui,R.R.; Carvalho,C.F.; Canola,J.C.; Vicente,W.R.R..
The aim of this study was to describe the use of acoustic radiation force impulse (ARFI) elastography in the evaluation of testicular disorders in dogs. Eighteen dogs with testicular disorders (thirty-six testicles) were assessed. Echotexture, size, contours and margins of testes were analysed by ultrasonography. Deformities and tissue stiffness (greyscale and homogenous or heterogeneous) were evaluated by qualitative elastography and shear velocity was determined quantitatively. Subsequent to orchiectomy, testicular samples were collected for histopathology analysis and thirty-six disorders were identified. Qualitative elastography revealed that normal healthy testicular tissues were homogenous and not pliable while the affected testicles had alterations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Stiffness; Testicular disease.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200283
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Carbohydrate antigen 125 predicts pulmonary congestion in patients with ST-segment elevation myocardial infarction BJMBR
Falcão,F.J.A.; Oliveira,F.R.A.; Cantarelli,F.; Cantarelli,R.; Brito-Júnior,P.; Lemos,H.; Silva,P.; Camboim,I.; Freire,M.C.; Carvalho,O.; Sobral-Filho,D.C..
Carbohydrate antigen 125 (CA125) has long been used as an ovarian cancer biomarker. However, because it is not specific for ovarian cells, CA125 could also be used to monitor congestion and inflammation in heart disease. Acute heart failure (HF) is used to identify patients with a worse prognosis in ST-segment elevation myocardial infarction (STEMI). We aimed to determine the association of CA125 with acute HF in STEMI and to compare CA125 with N-terminal pro brain natriuretic peptide (NTproBNP) with a cross-sectional study. At admission, patients were examined to define Killip class and then underwent coronary angioplasty. Blood samples, preferably taken in the hemodynamic ward, were centrifuged (1500 g for 15 min at ambient temperature) and stored at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute coronary syndrome; Pulmonary edema; CA125 antigen; Natriuretric peptide; Myocardial infarction.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200611
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Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity BJMBR
Mota,A.; Silva,P.; Neves,D.; Lemos,C.; Calhau,C.; Torres,D.; Martel,F.; Fraga,H.; Ribeiro,L.; Alçada,M.N.M.P.; Pinho,M.J.; Negrão,M.R.; Pedrosa,R.; Guerreiro,S.; Guimarães,J.T.; Azevedo,I.; Martins,M.J..
Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the p-nitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 ± 3.43 nmol p-nitrophenol·mg protein-1·min-1): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heart; Alkaline phosphatase; Polyphenol-rich beverages; Steroid hormones; Methylxanthines.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700009
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Dietary intake and eating behavior in depot medroxyprogesterone acetate users: a systematic review BJMBR
Silva,P.; Qadir,S.; Fernandes,A.; Bahamondes,L.; Peipert,J.F..
Because of weight gain, women often discontinue hormonal contraception, especially depot medroxyprogesterone acetate (DMPA). Our objective was to conduct a systematic review of studies describing dietary intake or eating behavior in DMPA users to understand whether the use of DMPA is associated with changes in dietary habits and behaviors leading to weight gain. We searched the PubMed, POPLINE, CENTRAL Cochrane, Web of Science, and EMBASE databases for reports published in English between 1980 and 2017 examining dietary intake or eating behavior in healthy women in reproductive age and adolescents using DMPA (150 mg/mL). Of the 749 publications screened, we excluded 742 due to duplicates (96), not addressing the key research question (638), not reporting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dietary intake; Eating behavior; Body weight; Female; Depot medroxyprogesterone acetate; Contraception.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600301
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Epitelização de enxertos cutâneos em feridas recentes de coelhos tratados com membrana amniótica canina e/ou laserterapia Arq. Bras. Med. Vet. Zootec.
Reis Filho,N.; Ferreira,M.; Pascoli,A.; Viéra,R.; Pazzini,J.; Oliveira,R.; Salardani,I.; Silva,P.; Rosseto,L.; Albernaz,S.; Vasconcelos,R.O.; Ferraudo,A.; Moraes,P.C.; Nardi,A..
RESUMO A enxertia cutânea é uma técnica cirúrgica simples e bastante útil para o reparo de feridas, principalmente aquelas onde existe dificuldade da aplicação do fechamento primário ou de outras técnicas reconstrutivas. Entretanto, para a sobrevivência do enxerto, é necessário que o leito da ferida esteja saudável e com presença de tecido de granulação exuberante. O objetivo do presente trabalho foi avaliar a aplicação da membrana amniótica e da laserterapia como potenciais estimulantes da cicatrização em enxertos aplicados em feridas sem tecido de granulação. Foram utilizados 42 coelhos, divididos em quatro grupos: grupo controle (GC), grupo membrana (GM), grupo laser (GL) e grupo membrana e laser (GML), submetidos a avaliações macro e microscópicas. Na...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epitelização; Enxerto cutâneo; Laserterapia; Membrana amniótica.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300603
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Pulmonar ARFI elastography and ultrasonography of canine fetal hydrops: case report Arq. Bras. Med. Vet. Zootec.
Maronezi,M.C.; Madruga,G.M.; Uscategui,R.A.R.; Simões,A.P.R.; Silva,P.; Rodrigues,M.G.K.; Cintra,C.A.; Assis,A.R.; Vicente,W.R.R.; Feliciano,M.A.R..
ABSTRACT This case report describes diagnostic and prognostic applicability of pulmonary acoustic radiation force impulse elastography and ultrasonography in canine hydrops fetalis. We also explore these methods’ potential in prediction of postnatal respiratory dysfunction. Two pregnant bitches (English bulldog [case 1] and French bulldog [case 2]) were referred for sonographic evaluation in their last week of pregnancy. Ultrasound showed that in each bitch, one fetus presented with lung alterations (hyperechogenicity, irregular surface, and pleural effusion) and anasarca. The other fetuses of the litter were normal, and they were observed as light gray and dark blue on pulmonary elastography. Their shear-wave velocity was 0.75m/s. Fetuses with hydrops...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Canine; Gestational ultrasonography; Fetal abnormalities.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501409
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Variabilidad del gen RYR1 y efecto en el pH de la carne en cerdos híbridos argentinos InVet
Marini,S.J.; Vanzetti,L.S.; Borelli,V.S.; Villareal,A.O.; Denegri,G.D.; Cottura,G.A.; Panichelli,D.; Silva,P.; Campagna,D.; Spiner,N.; Brunori,J.C.; Franco,R..
El gen RYR1 es la principal causa del síndrome de estrés porcino (PSS) y la presencia de carne pálida, blanda y exudativa (PSE) en los cerdos. En Argentina no existen informes sobre la variabilidad de dicho gen en cerdos híbridos y su efecto sobre la calidad de la canal. Por PCR-HRM se analizaron 403 cerdos híbridos de Argentina y el 65,0% eran homocigotos NN, 30.8% heterocigotos Nn y el 4,2% homocigotos nn. Además, se detectó un efecto significativo (p <0,0001) del gen RYR1 en el pH de la canal a los 45 minutos post faena. Los genotipos NN mostraron los mayores valores de pH a los 45 minutos después del sacrificio (6.27), los heterocigotos Nn mostraron valores intermedios de pH (5,94) y los homocigotos nn presentaron los valores más bajos de pH (5,64)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerdos; RYR1; Marcadores moleculares; PSE.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982012000100002
Registros recuperados: 8
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