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A model of chronic IgE-mediated food allergy in ovalbumin-sensitized mice BJMBR
Saldanha,J.C.S.; Gargiulo,D.L.; Silva,S.S.; Carmo-Pinto,F.H.; Andrade,M.C.; Alvarez-Leite,J.I.; Teixeira,M.M.; Cara,D.C..
Food allergy is most frequently the result of IgE-mediated hypersensitivity reactions. Here, we describe a chronic model in which some of the intestinal and systemic consequences of continuous egg white solution ingestion by ovalbumin-sensitized eight-week-old BALB/c mice, 6 animals per group, of both sexes, were investigated. There was a 20% loss of body weight that began one week after antigen exposure and persisted throughout the experiment (3 weeks). The sensitization procedure induced the production of anti-ovalbumin IgG1 and IgE, which were enhanced by oral antigen exposure (129% for IgG1 and 164% for IgE, compared to sensitization values). Intestinal changes were determined by jejunum edema at 6 h (45% Evans blue extravasation) and by a significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food allergy; Ovalbumin; Murine model; IgE; Hypersensitivity.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600005
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Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases Arq. Bras. Med. Vet. Zootec.
Santos,F.G.A.; Vasconcelos,A.C.; Nunes,J.E.S.; Cassali,G.D.; Paixão,T.A.; Martins,A.S.; Silva,S.S.; Martins,R.F.; Moro,L..
Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Venereal tumor; Apoptosis; Cell death.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300013
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Cell proliferation markers in the transplanted canine transmissible venereal tumor Arq. Bras. Med. Vet. Zootec.
Santos,F.G.A.; Moro,L.; Cassali,G.D.; Paixão,T.A.; Campos,P.P.; Silva,S.S.; Vasconcelos,A.C..
Adult male mongrel dogs were subcutaneously transplanted with the canine transmissible venereal tumor (TVT) on the hypogastric region. Twelve specimens of tumors were collected, half during the proliferative phase and the other half during the regressive phase. Fragments of the tumor were fixed in 10% buffered formalin and routinely processed for light microscopy. Sections of 4µm were stained by Schorr or AgNOR or either immunostained for MIB1 (Ki67). Schorr stain, AgNOR and MIB1 showed an increased proliferative activity through mitotic index, nuclear argyrophilic protein stain and cycling tumoral cells in the growing tumors, respectively. All of the three cell proliferation markers were able to distinguish the TVT in both evolution phases. MIB1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Venereal tumors; MIB1; AgNOR.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600010
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Immune and hormonal activity in adults suffering from depression BJMBR
Nunes,S.O.V.; Reiche,E.M.V.; Morimoto,H.K.; Matsuo,T.; Itano,E.N.; Xavier,E.C.D.; Yamashita,C.M.; Vieira,V.R.; Menoli,A.V.; Silva,S.S.; Costa,F.B.; Reiche,F.V.; Silva,F.L.V.; Kaminami,M.S..
An association between depression and altered immune and hormonal systems has been suggested by the results of many studies. In the present study we carried out immune and hormonal measurements in 40 non-medicated, ambulatory adult patients with depression determined by CID-10 criteria and compared with 34 healthy nondepressed subjects. The severity of the condition was determined with the Hamilton Depression Rating Scale. Of 40 depressed patients, 31 had very severe and 9 severe or moderate depression, 29 (72.5%) were females and 11 (27.5%) were males (2.6:1 ratio). The results revealed a significant reduction of albumin and elevation of alpha-1, alpha-2 and ß-globulins, and soluble IL-2 receptor in patients with depression compared to the values obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Depression; Immunity; Psychoneuroimmunology; Cytokines; Acute phase proteins.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500011
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