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An in vitro study of two GAG-like marine polysaccharides incorporated into injectable hydrogels for bone and cartilage tissue engineering ArchiMer
Rederstorff, Emilie; Weiss, Pierre; Sourice, S.; Pilet, P.; Xie, F.; Sinquin, Corinne; Colliec-jouault, Sylvia; Guicheux, Jerome; Laib, S..
Natural polysaccharides are attractive compounds with which to build scaffolds for bone and cartilage tissue engineering. Here we tested two non-standard ones, HE800 and GY785, for the two-dimensional (2-D) and three-dimensional (3-D) culture of osteoblasts (MC3T3-E1) and chondrocytes (C28/I2). These two glycosaminoglycan-like marine exopolysaccharides were incorporated into an injectable silylated hydroxypropylmethylcellulose-based hydrogel (Si-HPMC) that has already shown its suitability for bone and cartilage tissue engineering. Results showed that, similarly to hyaluronic acid (HA) (the control), HE800 and GY785 significantly improved the mechanical properties of the Si-HPMC hydrogel and induced the attachment of MC3T3-E1 and C28/I2 cells when these...
Tipo: Text Palavras-chave: Glycosaminoglycan; Hydrogel; Polysaccharide; Bone and cartilage tissue engineering; In vitro test.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00030/14090/15747.pdf
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Anti-Metastatic Properties of a Marine Bacterial Exopolysaccharide-Based Derivative Designed to Mimic Glycosaminoglycans ArchiMer
Heymann, Dominique; Ruiz-velasco, Carmen; Chesneau, Julie; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia.
Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no...
Tipo: Text Palavras-chave: Exopolysaccharides; Glycosaminoglycan; Heparin-like; Derivatives; Sulfation; Bone metabolism; Bone remodeling; Lung mestatases; Osteosarcoma.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00333/44442/44112.pdf
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Antiviral Activities of Sulfated Polysaccharides Isolated from Sphaerococcus coronopifolius (Rhodophytha, Gigartinales) and Boergeseniella thuyoides (Rhodophyta, Ceramiales) ArchiMer
Bouhlal, Rhimou; Haslin, Camille; Chermann, Jean-claude; Colliec-jouault, Sylvia; Sinquin, Corinne; Simon, Gaelle; Cerantola, Stephane; Riadi, Hassane; Bourgougnon, Nathalie.
Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 mu g/mL. In addition, polysaccharides were capable of inhibiting the in vitro replication of Herpes simplex virus type 1 (HSV-1) on Vero cells values of EC(50) of 4.1 and 17.2 mu g/mL, respectively. The adsorption step of HSV-1 to the host cell seems to be the specific target for polysaccharide action. While for HIV-1, these results suggest a direct inhibitory effect on HIV-1 replication by controlling the appearance of the new generations of virus and...
Tipo: Text Palavras-chave: Herpes simplex virus; Human immunodeficiency virus; Antiviral activity; Sulfated polysaccharide; S. coronopifolius; B. thuyoides; Chemical composition.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00060/17120/14631.pdf
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Assembly of HE800 exopolysaccharide produced by a deep-sea hydrothermal bacterium into microgels for protein delivery applications ArchiMer
Zykwinska, Agata; Marquis, Melanie; Sinquin, Corinne; Cuenot, Stephane; Colliec-jouault, Sylvia.
Assembly of biopolymers into microgels is an elegant strategy for bioencapsulation with various potential biomedical applications. Such biocompatible and biodegradable microassemblies are developed not only to protect the encapsulated molecule but also to ensure its sustained local delivery. The present study describes the fabrication of microassemblies from a marine HE800 exopolysaccharide (EPS), which displays a glycosaminoglycan (GAG)-like structure and biological properties. HE800 EPS was assembled, through physical cross-linking with divalent ions, into microgel particles and microfibers using microfluidics. The microparticle morphology was highly affected by the polysaccharide concentration and its molecular weight. A model protein, namely Bovine...
Tipo: Text Palavras-chave: Marine exopolysaccharide; Microgel particles; Microfibers; Microfluidic; Microencapsulation; Protein release.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00311/42175/41475.pdf
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Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides ArchiMer
Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M.
To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is...
Tipo: Text Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf
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Bioprospecting for Exopolysaccharides from Deep-Sea Hydrothermal Vent Bacteria: Relationship between Bacterial Diversity and Chemical Diversity ArchiMer
Delbarre-ladrat, Christine; Salas, Marcia Leyva; Sinquin, Corinne; Zykwinska, Agata; Colliec-jouault, Sylvia.
Many bacteria biosynthesize structurally diverse exopolysaccharides (EPS) and excrete them into their surrounding environment. The EPS functional features have found many applications in industries such as cosmetics and pharmaceutics. In particular, some EPS produced by marine bacteria are composed of uronic acids, neutral sugars, and N-acetylhexosamines, and may also bear some functional sulfate groups. This suggests that they can share common structural features with glycosaminoglycans (GAG) like the two EPS (HE800 and GY785) originating from the deep sea. In an attempt to discover new EPS that may be promising candidates as GAG-mimetics, fifty-one marine bacterial strains originating from deep-sea hydrothermal vents were screened. The analysis of the...
Tipo: Text Palavras-chave: Exopolysaccharides; GAG; Marine bacteria; Glycopolymers; Sulfate; Production.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00399/51090/51873.pdf
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Characterization, chemical modifications and in vitro anticoagulant properties of an exopolysaccharide produced by Alteromonas infernus ArchiMer
Colliec-jouault, Sylvia; Chevolot, Lionel; Helley, Dominique; Ratiskol, Jacqueline; Bros, Andrée; Sinquin, Corinne; Roger, Olivier; Fischer, Anne-marie.
A new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. Data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. Two depolymerization processes were used to obtain low-molecular-weight (24-35 x 10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%), Nuclear magnetic resonance studies indicated that after sulfation (40%), the low-molecular-weight fraction obtained by free radical depolymerization was less sulfated in the 6-O-position than the fraction depolymerized by acid hydrolysis. The free radical depolymerized product also had sulfated residues in...
Tipo: Text Palavras-chave: Sulfation; Sulfated polysaccharide; Serpin; Heparin like; Depolymerization; Anticoagulant activity; Affinity co electrophoresis.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-2111.pdf
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Characterization of biofilm extracts from two marine bacteria ArchiMer
Passerini, Delphine; Fecamp, Florian; Marchand, Laetitia; Kolypczuk, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Verrez-bagnis, Veronique; Hervio Heath, Dominique; Colliec Jouault, Sylvia; Delbarre Ladrat, Christine.
In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were...
Tipo: Text Palavras-chave: Vibrio; Biofilm; Polysaccharide; Quorum-sensing; Activity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00592/70381/68461.pdf
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Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract ArchiMer
Akoumany, Katy; Zykwinska, Agata; Sinquin, Corinne; Marchand, Laetitia; Fanuel, Mathieu; Ropartz, David; Rogniaux, Hélène; Pipelier, Muriel; Delbarre Ladrat, Christine; Colliec Jouault, Sylvia.
Bacteria from deep-sea hydrothermal vents constitute an attractive source of bioactive molecules. In particular, exopolysaccharides (EPS) produced by these bacteria become a renewable source of both biocompatible and biodegradable molecules. The low molecular weight (LMW) derivatives of the GY785 EPS produced by the deep-sea hydrothermal vent strain Alteromonas infernus have previously displayed some biological properties, similar to those of glycosaminoglycans (GAG), explored in cancer and tissue engineering. These GAG-mimetic derivatives are obtained through a free radical depolymerization process, which could, however, affect their structural integrity. In a previous study, we have shown that A. infernus produces depolymerizing enzymes active on its own...
Tipo: Text Palavras-chave: Deep-sea bacterium; Alteromonas infernus; Wild-type strain; Exopolysaccharides; Glycosaminoglycan-mimetic; Enzymatic depolymerization; Structural analysis; Mass spectrometry.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00515/62644/67021.pdf
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Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities ArchiMer
Balti, Rafik; Le Balc'H, Romain; Brodu, Nicolas; Gilbert, Marthe; Le Gouic, Benjamin; Le Gall, Sophie; Sinquin, Corinne; Masse, Anthony.
Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or...
Tipo: Text Palavras-chave: Membrane filtration; Exopolysaccharides; Microalga; Concentration; Diafiltration.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00445/55694/57360.pdf
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Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides ArchiMer
Zykwinska, Agata; Marchand, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment. The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS). EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve. Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups. Such a particular composition ensures interesting functional properties, including biological activities mimicking...
Tipo: Text Palavras-chave: Exopolysaccharide; Glycosaminoglycan; Uronic acid; Hexosamine; Phylogenetic analysis.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00506/61756/65728.pdf
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Effect of an oversulfated exopolysaccharide on angiogenesis induced by fibroblast growth factor-2 or vascular endothelial growth factor in vitro ArchiMer
Matou, Sabine; Colliec-jouault, Sylvia; Galy Fauroux, Isabelle; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, Anne-marie; Helley, Dominique.
The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6)...
Tipo: Text Palavras-chave: Matrigel; Collagen gel; Alpha 6 integrin subunit; Angiogenic growth factor; Polysaccharide; Angiogenesis.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2118.pdf
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Effect of low-molecular-weight fucoidan on experimental arterial thrombosis in the rabbit and rat ArchiMer
Colliec-jouault, Sylvia; Millet, J; Helley, D; Sinquin, Corinne; Fischer, Am.
Tipo: Text Palavras-chave: Arterial thrombosis; Polyaccharide; Fucoidan.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-2116.pdf
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Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology ArchiMer
Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M..
The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the...
Tipo: Text Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf
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Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10 ArchiMer
Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...
Tipo: Text Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf
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Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10 ArchiMer
Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...
Tipo: Text Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00372/48356/48545.pdf
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Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment ArchiMer
Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened...
Tipo: Text Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf
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Exopolysaccharides produced by bacteria isolated from deep-sea hydrothermal vents: new agents with therapeutic potential ArchiMer
Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean.
Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new...
Tipo: Text Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf
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Exopolysaccharides produced by marine bacteria and their applications as glycosaminoglycan-like molecules ArchiMer
Delbarre-ladrat, Christine; Sinquin, Corinne; Lebellenger, Lou; Zykwinska, Agata; Colliec-jouault, Sylvia.
Although polysaccharides are ubiquitous and the most abundant renewable bio-components, their studies, covered by the glycochemistry and glycobiology fields, remain a challenge due to their high molecular diversity and complexity. Polysaccharides are industrially used in food products; human therapeutics fall into a more recent research field and pharmaceutical industry is looking for more and more molecules with enhanced activities. Glycosaminoglycans (GAGs) found in animal tissues play a critical role in cellular physiological and pathological processes as they bind many cellular components. Therefore, they present a great potential for the design and preparation of therapeutic drugs. On the other hand, microorganisms producing exopolysaccharides (EPS)...
Tipo: Text Palavras-chave: Marine-derivedpolysaccharide; Biodiversity; Biologicalactivity; Structure; Modification; GAG-mimetic; Biosynthesis.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00241/35250/33764.pdf
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Free-radical depolymerization with metallic catalysts of an exopolysaccharide produced by a bacterium isolated from a deep-sea hydrothermal vent polychaete annelid ArchiMer
Petit, Anne-celine; Noiret, N; Sinquin, Corinne; Ratiskol, Jacqueline; Guezennec, Jean; Colliec-jouault, Sylvia.
Free-radical depolymerization with metallic catalysts has been investigated to depolymerize an exopolysaccharide (EPS), with a high molecular weight (> 10(6) g/Mol), produced by the bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. Three metals (copper, zinc and manganese) were used either alone or mixed. These metals led to the depolymerization of the high molecular weight bacterial polysaccharide with different efficiency. This study specifies some conditions required to produce, with good reproducibility and yield, EPS derivatives of reduced molecular weight ranging from 100,000 to 20,000 g/mol. The more influential parameters are specified. This is a first study to find suitable industrial conditions in the presence of these metallic...
Tipo: Text Palavras-chave: Bacterial exopolysaccharide; Free radical depolymerization; H202; Metal.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2146.pdf
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