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| Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek. |
| A flow cytometry protocol was applied for the detection of neoplasia in Macoma balthica L. from the Gulf of Gdansk (Baltic Sea, Poland). A simple method, based on an osmotic shock, was used to permeabilise gill cells. The cytometric pattern of normal clams consisted of 2 peaks, a major peak B and a smaller peak C. The cytometric pattern of affected clams consisted of 2 peaks named B' and C'. Two parameters were used to define the stages of abnormalities in M. balthica clams based on the percentage of cells in peaks B, C, B' and C' and on the ratio between the fluorescence value of peaks B, C, B' and C' in all individuals. Three stages of neoplasia were clearly distinguished by flow cytometry considering peak C'. Stage 1 was characterised by a major... |
| Tipo: Text |
Palavras-chave: Baltic Sea; Macoma balthica; Neoplasia; Cytogenetics; Histology; Flow cytometry. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2918.pdf |
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| Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek. |
| Using flow cytometry, 234 Macoma balthica were examined during a survey to determine frequency of neoplasia in the Gulf of Gdansk (Poland). Clams were collected in 4 locations and DNA content in gill tissue cells was determined by flow cytometry using propidium iodide staining. Cell permeabilization was induced by osmotic shock. Prevalence of neoplasia ranged from 9.6 to 26.7 % depending on location. DNA content in aneuploid cells was higher than in normal dividing cells. The fluorescence value for aneuploid cells corresponded to tetraploid/pentaploid cells. Three stages of neoplasia were defined, based on the percentage of aneuploid cells determined by flow cytometry. Histopathological and cytogenetic analyses were also carried out on the same clams for... |
| Tipo: Text |
Palavras-chave: Prevalence; Macoma balthica; Flow cytometry; Gill neoplasia. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2919.pdf |
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| Smolarz, Kasia; Renault, Tristan; Wolowicz, Maciej. |
| In the Baltic clam Macoma balthica from the Gulf of Gdansk, neoplasia is considered as a serious epizootic linked to bad environmental conditions and high levels of pollutants. Previous research was focused on the diagnosis, prevalence, seasonality and histopathological characteristic of the cancer. This study is focused on electron microscopy analyses describing cell ultrastructure abnormalities related to neoplasia. Examinations using the electron microscopy highlighted changes confined to anatomic ultrastructures, shapes and functions of neoplastic cells. The lobulated appearance of the nucleus, changes in cellular matrix and the occurrence of large granular cells with hyperchromatic nuclei, atypical Golgi structures and deterioration of rough... |
| Tipo: Text |
Palavras-chave: Gulf of Gdansk; Macoma balthica; Neoplasia; Myofibroblast like cells; Cell ultrastructures. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1689.pdf |
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