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| TISKI, I.; PEREIRA, L. F. P.; POT, D.; MARRACCINI, P. R.; VIEIRA, L. G. E.. |
| Os diterpenos cafestol e caveol, presentes na fração lipídica em grãos de café, originam-se da via de síntese de isoprenóides. Estes compostos são sintetizados em todos os organismos, sendo abundantes em plantas com cerca de 10 mil componentes relatados. Apesar da diversidade de funções e estruturas todos os isoprenóides derivam de cinco comuns átomos de carbono, o isopentenil difosfato (IPP) e do isômero dimetilalil difosfato (DMAPP). Em vegetais superiores, duas vias localizadas em compartimentos intracelulares separados estão envolvidas na biossíntese de IPP e DMAPP. No citosol, IPP é derivado da via do ácido mevalônico (MVA) e no plastídeo, IPP é formado pela via do metileritritol fosfato (MEP ou não mevalonato). Com a disponibilidade das seqüências... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Coffee; Analysis; In silico; In vivo; MEP; MVA.; Análise; Café.; Coffea.. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/906049 |
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| TISKI, I.; MARRACCINI, P.; POT, D.; VIEIRA, L. G.; PEREIRA, L. F. P.. |
| In higher plants there are two independent pathways for isoprenoid biosynthesis, located in the cytosol (mevalonic acid or MVA pathway) or in the plastids (methylerythritol phosphate ? MEP pathway). The 3-hydroxy-3-methyglutaryl-CoA reductase (HMGR) is the first committed step in the MVA pathway. Using the information available from the Brazilian Coffee Genome Project, we found 13 ESTs that originated two isoforms, CaHMGR1 and CaHMGR2, for the enzyme HMGR of Coffea arabica. A complementary DNA encoding the isoform CaHMGR1 was cloned, and its complete nucleotide sequence determined. The full-length cDNA of CaHMGR1 was 2242 bp containing a 1812-bp ORF encoding 604 amino acids. Bioinformatic analyses revealed that the deduced CaHMGR1 had extensive homology... |
| Tipo: Artigo de periódico |
Palavras-chave: Ydroxy.; Molecular characterization; Expressed sequence tag (EST); Methyglutaryl-CoA reductase; Coffee; Gene expression; Sequence analysis; Isoprenoids.. |
| Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/902481 |
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| TISKI, I.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; TAKAHASHI, E. K.; FERREIRA, L. P.; MARRACCINI, P.; POT, D.; LEROY, T.; BENASSI, M. T.; DIAS, R. C. E.. |
| The most important lipids in coffee, the diterpenes khaweol and cafestol, are originated from the isoprenoid pathway. Despite their diversity in functions and structures, all isoprenoids derive from the common-five carbon building unit isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In higher plants, there are two independent pathways located in the cytosol (mevalonic acid or MVA pathway) and in the plastids (methylerythritol phosphate ? MEP ? or non mevalonic pathway). Throughout the data mining of the Brazilian Coffee Genome Project we studied the genes that code for the enzymes 3-hydroxy-3-methyglutaryl-CoA reductase (HMGR) and mevalonate diphosphate decarboxylase (MPDC) for the MVA pathway and 1-deoxy-D-xylulose... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Coffee; Lipid in coffee.. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/885763 |
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