Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 28
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) ArchiMer
Taris, Nicolas; Baron, S; Sharbel, Tim; Sauvage, Christopher; Boudry, Pierre.
The analysis of parentage using microsatellite markers is of increasing importance, notably in aquaculture genetic research where communal rearing of mixed families can be used to reduce unwanted environmental variance. We present here an optimization of parental genotype assessment for larvae or adults of the Pacific oyster, Crassostrea gigas, using a multiplex system of three microsatellite loci. In conjunction with a simple DNA extraction protocol, this method enables high throughput analyses of parentage in C. gigas. Using this method, we successfully determined the parentage of 93% (1224 out of 1318) of the progeny in a factorial cross between 3 females and 10 males. The inability to genotype the remaining 7% was due to DNA degradation of larvae...
Tipo: Text Palavras-chave: Oysters; Aquaculture; Parental assignment; High throughput; Larval DNA extraction; Multiplex.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1814.pdf
Imagem não selecionada

Imprime registro no formato completo
Comparative study of shell shape and muscle scar pigmentation in the closely related cupped oysters Crassostrea angulata, C. gigas and their reciprocal hybrids ArchiMer
Batista, Frederico; Ben-hamadou, Radhouan; Fonseca, Vera; Taris, Nicolas; Ruano, Francisco; Reis-henriques, Maria; Boudry, Pierre.
The taxonomic status of the cupped oysters Crassostrea angulata and C. gigas has received considerable attention in the last decades. Based on larval shell morphology, experimental hybridization, allozymes and nuclear DNA studies several authors have considered these two taxa as being synonymous. However, mitochondrial data showed clear genetic differences between the two taxa. In addition, microsatellite-based studies and cytogenetic studies have also provided evidence that supports their differentiation. Considerable differences have also been observed at the phenotypic level in terms of growth rate and ecophysiological parameters. In the present study, C. angulata from Sado estuary (Portugal) and C. gigas from Seudre estuary (France) were collected and...
Tipo: Text Palavras-chave: Crassostrea gigas; Crassostrea angulata; Hybridization; Shell pigmentation; Shell morphology.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3904.pdf
Imagem não selecionada

Imprime registro no formato completo
Conséquences génétiques de la production de larves d'huîtres en écloserie : étude des processus de dérive et de sélection ArchiMer
Taris, Nicolas; Sauvage, Christopher; Batista, Frederico; Baron, Sophie; Ernande, Bruno; Haffray, Pierrick; Boudry, Pierre.
Previous studies have shown heritable variation in larval developmental traits in the Pacific oyster Crassostrea gigas. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors, specific to hatcheries, were examined: the effect of discarding the smallest larvae (i.e. culling) and the effect of temperature (20°C versus 26°C). A mixed-family approach was used in order to infer the genetic composition of larval populations and family assignment, limiting possible environmental bias and allowing the study of a relatively large number of families using a limited number of larval tanks. Our results show that three multiplexed highly polymorphic microsatellite markers are a powerful tool for family assignment and,...
Tipo: Text Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-1505.pdf
Imagem não selecionada

Imprime registro no formato completo
Conséquences génétiques de la production intensive de larves d'huîtres en écloserie : étude des processus de dérive et de sélection liés aux pratiques d'élevage. ArchiMer
Taris, Nicolas.
In order to study the genetic influence of intensive production of the larval stage in commercial hatcheries, two types of specific selective processes were examined: the effects of discarding the smallest larvae and temperature. A mixed-family approach was used in order to infer the genetic composition of the larvae. The results show that our mixed-family approach, combined with microsatellite-based family assignment, is a powerful tool for the study of bivalve larvae genetics. Selective sieving is an advantageous practice at a phenotypic scale, but also represents a substantial risk for diversity loss. The culled larvae are important for the minimization of the variability in reproductive success and the maximization of broodstock genetic diversity....
Tipo: Text Palavras-chave: Hatchery; Oyster; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Huître; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-976.pdf
Imagem não selecionada

Imprime registro no formato completo
Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae ArchiMer
Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Tipo: Text Palavras-chave: DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2916.pdf
Imagem não selecionada

Imprime registro no formato completo
Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes) ArchiMer
Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,...
Tipo: Text Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf
Imagem não selecionada

Imprime registro no formato completo
Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L. ArchiMer
Lapegue, Sylvie; Boudry, Pierre; Mira, Sara; Taris, Nicolas; Bonhomme, Francois.
In order to understand better some fundamental aspects of the bivalve biology, one has to pay attention to the variance of their breeding success, the time structure of the populations and their effective size. An abundant literature shows the existence of a heterozygote deficit as well as positive relationships between heterozygoty and fitness components. This last point is the subject of explanatory hypotheses which have never been clearly decided between until now. One of these hypotheses is the possible existence of a certain degree of inbreeding among those populations. This is only possible in the case of separated colonies coming from a small amount of spawners, thus creating blood relations between individuals recruited from the same area, and...
Tipo: Text Palavras-chave: Reproducteur; Variabilité génétique; Génétique population; Diversité génétique; Ostrea edulis; Huître plate.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-3494.pdf
Imagem não selecionada

Imprime registro no formato completo
Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L. 2002 ArchiMer
Taris, Nicolas.
Cette étude aborde la diversité génétique d'une espèce de bivalve marin, Ostrea edulis et plus particulièrement les interactions existant entre génétique (flux de gènes) et démographie (dynamique de reproduction) à l'échelle de la population. Elle repose sur l'utilisation de marqueurs microsatellites pour tenter de comprendre les facteurs participant à la mise ne place d'une structuration génétique au sein de laquelle peuvent être observés des phénomènes de déficit en hétérozygotes et de ration positive entre hétérozygotie et valeur sélective. L'un des objectifs est en particulier de tester l'hypothèse de consanguinité à l'échelle locale. Pour cela, nous avons étudier la structure génétique de cohortes d'huîtres plates au cours d'une saison de reproduction...
Tipo: Text Palavras-chave: Ostrea edulis; Génétique des Populations; Microsatellites; Diversité génétique; Dynamique de recrutement.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00033/14419/11714.pdf
Imagem não selecionada

Imprime registro no formato completo
Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L. (2003) ArchiMer
Lapegue, Sylvie; Taris, Nicolas; Boudry, Pierre; Langlade, Aime; Bonhomme, Francois.
Le milieu marin est un milieu difficile à appréhender car composé d'un ensemble d'organismes et d'écosystèmes caractérisés par des conditions de fonctionnement tout à fait originales et souvent difficiles d'accès. Ces spécificités peuvent être attribuées à la structure spatiale du milieu marin, qui à bien des égards, diffère du milieu terrestre. En effet si les notions de barrières géographiques sont une réalité concrète pour les organismes terrestres, elles restent rarement identifiées en milieu marin (PALUMBI 1994). Dans un tel environnement, les organismes présentent des cycles de vie souvent mal connus, notamment ceux qui associent phase benthique et phase planctonique. L'étude de la structure génétique des populations, dans un espace aussi ouvert aux...
Tipo: Text Palavras-chave: Diversité génétique; Génétique; Recrutement; Génotypage; Variabilité génétique; Huître plate; Ostrea edulis.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00044/15478/12857.pdf
Imagem não selecionada

Imprime registro no formato completo
Effects of genetic drift and selection at larval stage resulting from hatchery practices in the Pacific oyster (Crassostrea gigas) ArchiMer
Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Batista, Frederico; Boudry, Pierre.
Hatchery techniques are now well handled in an increasing number of aquaculture species. However, unintentional effects of domestication often remain poorly documented. In this context, we studied how current hatchery practices may genetically influence larval traits for which significant genetic variation had previously been reported in the Pacific oyster. Two main factors were studied: culling (i.e. discarding the smallest larvae) and rearing temperature (26°C versus 20°C). In parallel, we estimated genetic diversity of broodstock populations sampled in French hatcheries. Finally, we compared larval development and settlement success in the progeny of one of these hatchery broodstock, which had been selected for seven generations, with those of wild...
Tipo: Text Palavras-chave: Genetic drift; Selection; Genetic; Crassostrea gigas; Oysters; Hatchery techniques.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3362.pdf
Imagem não selecionada

Imprime registro no formato completo
Etude de la reproduction de l'huitre creuse dans le Bassin d'Arcachon - Année 2001 ArchiMer
Auby, Isabelle; Maurer, Daniele; Masson, Nadine; Durand, Nathalie; Sautour, Benoit; Taris, Nicolas; D'Amico, Florence; Guillard, Fabien; Guegen, Cathy; Trut, Gilles; Cantin, Christian; Pellier, Claude.
Tipo: Text
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00083/19395/16988.pdf
Imagem não selecionada

Imprime registro no formato completo
Evidence of response to unintentional selection for faster development and inbreeding depression in Crassostrea gigas larvae ArchiMer
Taris, Nicolas; Batista, F; Boudry, Pierre.
Underlying consequences of domestication and artificial selection still remain largely unexplored in most aquacultured species. For species with a two phase life cycle, including the Pacific oyster Crassostrea gigas, most genetic studies have focused on the post-metamorphosis juvenile and adult stages, but relatively few considered the larval stage. To assess the consequence of hatchery practices on larval characters, especially growth, we performed a phenotypic study on larval progenies derived from crosses between Pacific oysters from natural beds and farmed Pacific oysters selected for desirable production traits such as rapid growth, for over seven generations. A set of three microsatellite loci was used to compare the genetic variability between the...
Tipo: Text Palavras-chave: Crassostrea gigas; Inbreeding depression; Selection; Domestication; Larval stage.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3848.pdf
Imagem não selecionada

Imprime registro no formato completo
Genetic effect of domestication selective pressures on Pacific oyster at larval stage ArchiMer
Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Among bivalve species, the Pacific oyster, Crassostrea gigas, is the most economically important bivalve production over the world. Today, C. gigas is subject to an important production effort that leads to an intensive artificial selection. Larval stage is relatively unknown, specifically in a domestication context. Genetic consequence of artificial selection is still at a preliminary study. We aimed to tackle the consequence of inconscient domestication on the variance reproductive success focusing on larval stage, keystone of the life cycle. We studied two kinds of specific selective processes that common hatchery rearing practices exert : the effect of discarding the smallest larvae on genetic diversity and the artificial environment rearing effect via...
Tipo: Text Palavras-chave: Selection; Genetic correlations; Genetic variability; Genetic; Domestication selective; Crassostrea gigas; Pacific oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3441.pdf
Imagem não selecionada

Imprime registro no formato completo
Genetic impact of the reproduction dynamics in the European flat oyster Ostrea edulis ArchiMer
Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual...
Tipo: Text Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf
Imagem não selecionada

Imprime registro no formato completo
Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) ArchiMer
Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
The most significant genetic improvement for production of Pacific oyster (Crassastrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have slowed the development of selective breeding programs. Recently, in the USA, Australia and New Zealand, family-based selective breeding programs have been initiated to improve growth and yield. In Europe, where both natural and...
Tipo: Text Palavras-chave: Microsatellites; Quantitative genetic; Genetic variability; Crassostrea gigas; Oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2861.pdf
Imagem não selecionada

Imprime registro no formato completo
Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) (slides) ArchiMer
Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective...
Tipo: Text Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf
Imagem não selecionada

Imprime registro no formato completo
Hybridization and morphological differences between the two closely related oyster taxa Crassostrea angulata and C. gigas ArchiMer
Batista, Frederico; Fonseca, Vera; Ben-hamadou, Radhouan; Taris, Nicolas; Henriques, Maria; Boudry, Pierre.
Unlike in some crops, no large heterotic effects have been observed in most farmed animals. Interestingly, significant hybrid vigour for some traits has been reported in the Pacific oyster, Crassostrea gigas. Crossbreeding between closely related taxa can be seen as an efficient way to exploit the dominance component of the genetic variance of a trait. Factorial crosses between the Portuguese oyster C. angulata (from Sado estuary, Portugal) and C. gigas (from Seudre estuary, France) were done to evaluate the aquaculture potential of hybrids. Juveniles of the different progenies were reared in Ria Formosa (Portugal) under usual farming conditions. The genetic confirmation of all progenies was done using mitochondrial and nuclear markers in order to detect...
Tipo: Text Palavras-chave: Hybridization; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3429.pdf
Imagem não selecionada

Imprime registro no formato completo
Impact génétique de la production intensive d'huîtres en écloserie : processus de dérive et de sélection liés aux pratiques d'élevage larvaire ArchiMer
Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Oysters, like many other marine bivalves, are characterized by high fertility and low survival rates during the precocious stages. Hatchery reproduction, realised in very different conditions than in their natural habitat (density, food, temperature, etc.), tends to optimize the production of larvae and juveniles. As a consequence, slow growing larvae are systematically culled. Previous results suggest the existence of a genetic variability linked to precocious development. Our approach consists in studying the evolutionary process (genetic drift and selection) linked with hatchery reproduction. Thanks to microsatellite markers enabling us to analyse the relatedness in "mixed" cultivated families, we studied the variability of breeding success in...
Tipo: Text Palavras-chave: Crassostrea gigas; Huîtres; Marqueurs microsatellites; Reproduction; Sélection; Dérive génétique; Variabilité génétique; Génétique.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3481.pdf
Imagem não selecionada

Imprime registro no formato completo
Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (Poster) ArchiMer
Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Early-life history of oysters, like in most marine bivalves, is characterized by high fecundity and low survival rate. In hatcheries, which are ecoming more and more important for the production of juveniles for aquaculture production, rearing conditions are optimised (density, feeding, temperature...), and contrast with those observed in the wild. Additionally, slow growing larvae are culled by sieving.
Tipo: Text Palavras-chave: Crassostrea gigas; Juvenile; Genetic variability; Selection; Genetic; Hatcheries; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3444.pdf
Imagem não selecionada

Imprime registro no formato completo
Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (slides) ArchiMer
Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Oysters, like many marine bivalves, are characterized by very high fecundity and low survival rate at early stages of life. Rearing conditions in hatcheries (i.e. density, feeding, temperature...) are different from those observed in the wild. Additionally, slow growing larvae are culled by sieving. As previous studies have shown that genetic variability exists for several early development traits, we aimed to assess if common hatchery rearing practices exert specific selective processes at the larval stage. In order to avoid possible bias and to increase the number of families studied, we used a mixed-family approach with subsequent parentage analysis using a recently developed set of three multiplexed microsatellite markers. We studied the variance in...
Tipo: Text Palavras-chave: Hatcheries; Selection; Genetic drift; Genetic; Crassotrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3483.pdf
Registros recuperados: 28
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional