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Tatry, M.V.; El Kassis, E.; Lambilliotte, R.; Corratgé, C.; van Aarle, I.; Amenc, L.; Alary, R.; Zimmermann, S.; Sentenac, H.; Plassard, C.. |
Ectomycorrhizal symbiosis markedly improves plant phosphate uptake, but the molecular mechanisms underlying this benefit are still poorly understood. We identified two ESTs in a cDNA library prepared from the ectomycorrhizal basidiomycete Hebeloma cylindrosporum with significant similarities to phosphate transporters from the endomycorrhizal fungus Glomus versiforme and from non-mycorrhizal fungi. The full-length cDNAs corresponding to these two ESTs complemented a yeast phosphate transport mutant (Dpho84). Measurements of 33P-phosphate influx into yeast expressing either cDNA demonstrated that the encoded proteins, named HcPT1 and HcPT2, were able to mediate Pi:H+ symport with different affinities for Pi (Km values of 55 and 4 lM, respectively). Real-time... |
Tipo: Journal Article |
Palavras-chave: PHOSPHATE TRANSPORTER; HETEROLOGOUS CHARACTERIZATION; EXPRESSION; SOIL P AVAILABILITY; PHOSPHATE UPTAKE; ECTOMYCORRHIZAL SYMBIOSIS. |
Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20095ee82dc1&uri=/notices/prodinra1/2010/03/ |
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