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| Ngoc Bui, Van; Thu Huyen Nguyen, Thi; Thanh Mai, Chi; Bettarel, Yvan; Yen Hoang, Thi; Thuy Linh Trinh, Thi; Hai Truong, Nam; Ha Chu, Hoang; Thanh Thanh Nguyen, Vu; Duc Nguyen, Huu; Woelfl, Stefan. |
| In Vietnam, a great number of toxic substances, including carcinogens and procarcinogens, from industrial and agricultural activities, food production, and healthcare services are daily released into the environment. In the present study, we report the development of novel yeast-based biosensor systems to determine both genotoxic carcinogens and procarcinogens by cotransformation with two plasmids. One plasmid is carrying human CPR and CYP (CYP3A4, CYP2B6, or CYP2D6) genes, while the other contains the RAD54-GFP reporter construct. The three resulting coexpression systems bearing both CPR-CYP and RAD54-GFP expression cassettes were designated as CYP3A4/CYP2B6/CYP2D6 + RAD54 systems, respectively and used to detect and evaluate the genotoxic potential of... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00625/73740/74114.pdf |
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