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Cross-amplification of heterologous microsatellite markers in Piracanjuba Ciência Rural
Castro,Pedro Luiz de; Ribeiro,Ricardo Pereira; Santos,Silvio Carlos Alves dos; Goes,Elenice Souza dos Reis; Souza,Felipe Pinheiro de; Poveda-Parra,Angela Rocio; Vargas,Lauro; Urrea-Rojas,Angela Maria; Lopera-Barrero,Nelson Mauricio.
ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brycon orbignyanus; Conservation; Molecular markers; Heterologous primers.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402
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Transferability of heterologous microsatellite primers in Brycon gouldingi Ciência Rural
Souza,Felipe Pinheiro de; Lima,Ed Christian Suzuki de; Leite,Natalia Gonçalves; Urrea-Rojas,Angela Maria; Yamachita,Andrei Lincoln; Pandolfi,Victor César Freitas; Lopera-Barrero,Nelson Mauricio.
ABSTRACT: Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Cross-amplification; Molecular markers; SSR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100406
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