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| PAES, R. de C. da S.; FONSECA JUNIOR, A. A.; MONTEIRO, L. A. R. C.; JARDIM, G. C.; PIOVEZAN, U.; HERRERA, H. M.; MAURO, R. A.; VIEIRA-da-MOTTA, O.. |
| The feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Feral swine; PCR; Glycoprotein; ELISA; Seroneutralization assay. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/971371 |
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