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	Provedor de dados BJMBR (2) Autor Valenzuela,M.A. (2) Alberti,C. (1) Cartier,L. (1) Espinosa,V. (1) Kettlun,A.M. (1) Rodriguez,L. (1) Zanocco,A. (1) Mais... Palavra-chave ATP-diphosphohydrolase (1) CTLA-4 (1) Desirée and Pimpernel isoapyrases (1) Foxp3 (1) GITR mRNA expression (1) HTLV-1-associated myelopathy/tropical spastic paraparesis (1) Intrinsic fluorescence (1) Potato apyrase (1) Quenching (1) Tax (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última In vivo fluctuation of Tax, Foxp3, CTLA-4, and GITR mRNA expression in CD4+CD25+ T cells of patients with human T-lymphotropic virus type 1-associated myelopathy BJMBR Cartier,L.; Rodriguez,L.; Alberti,C.; Valenzuela,M.A.. HTLV-1 Tax expression exerts an inhibitory effect on the Foxp3 transcription factor in CD4+CD25+ T-regulatory cells (Treg). For a better understanding of the role of Tax mRNA in the gene expression of cellular markers we measured Tax, Foxp3, CTLA-4, GITR, TGF-β, and IL-10 mRNA in Treg cells of 50 patients with human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP; 27 women and 23 men; mean age: 56.7 years). The control group consisted of 23 non-infected subjects (12 women and 11 men) with a mean age of 51.3 years. Real-time PCR was used to measure mRNA of Tax proteins and several cellular markers of Treg function. Determinations revealed a high level of Tax mRNA in HAM/TSP (124.35 copies/100 CD4+CD25+ T... Tipo: Info:eu-repo/semantics/article Palavras-chave: HTLV-1-associated myelopathy/tropical spastic paraparesis; Tax; Foxp3; CTLA-4; GITR mRNA expression. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100014 Studies on ATP-diphosphohydrolase nucleotide-binding sites by intrinsic fluorescence BJMBR Kettlun,A.M.; Espinosa,V.; Zanocco,A.; Valenzuela,M.A.. Potato apyrase, a soluble ATP-diphosphohydrolase, was purified to homogeneity from several clonal varieties of Solanum tuberosum. Depending on the source of the enzyme, differences in kinetic and physicochemical properties have been described, which cannot be explained by the amino acid residues present in the active site. In order to understand the different kinetic behavior of the Pimpernel (ATPase/ADPase = 10) and Desirée (ATPase/ADPase = 1) isoenzymes, the nucleotide-binding site of these apyrases was explored using the intrinsic fluorescence of tryptophan. The intrinsic fluorescence of the two apyrases was slightly different. The maximum emission wavelengths of the Desirée and Pimpernel enzymes were 336 and 340 nm, respectively, suggesting small... Tipo: Info:eu-repo/semantics/article Palavras-chave: Potato apyrase; ATP-diphosphohydrolase; Intrinsic fluorescence; Quenching; Desirée and Pimpernel isoapyrases. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700001 Registros recuperados: 2 Primeira ... 1 ... Última

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