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The use of biomarkers in <i>Daphnia magna</i> testing: 3. Rapid toxicity testing of pure chemicals and sediment pore waters using ingestion and digestive enzyme activity 27
De Coen, W.M.; Vangheluwe, M.L.; Janssen, C.R..
In this study, 4 rapid (90 min) toxicity tests were developed using ingestion and digestive enzyme activity of <i>Daphnia magna</i> juveniles. Ingestion activity was assessed using fluorescent labelled latex micro-beads and digestive enzyme activity (trypsin, beta-galactosidase and esterase) was measured in homogenates using chromogenic (N-benzoyl-L-arginine-4-nitroanilide) and fluorogenic (4-methylumbelliferyl-B-D-galactoside and fluorescein diacetate) substrates.All assays and toxicity endpoints were evaluated for their potential use as routine toxicity testing tools for pure chemicals and sediment pore waters. The observed high correlation coefficients (r(2)>0.9) between the short-term toxicity values and the acute toxicity endpoint (24h...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Assimilation; Assimilation; Exposure; Exposure; Filtration; Respiration; Zooplankton; Daphnia magna; Rotifera.
Ano: 1998 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=7488
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