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Comparison of agglutinating and neutralizing antibodies to serovar hardjo in sows immunized with two commercial whole culture polivalent anti-leptospira bacterins BJM
Soto,Francisco Rafael Martins; Pinheiro,Sônia Regina; Morais,Zenaide Maria; Gonçales,Amane Paldês; Azevedo,Sérgio Santos de; Bernardi,Fernanda; Camargo,Sebastião Rodrigues; Vasconcellos,Silvio Arruda.
It was performed the comparison of the intensity and duration of agglutinating and neutralizing antibodies to serovar Hardjo in swines vaccinated with two commercial anti-leptospira bacterins. Sows no reactive to 24 Leptospira sp serovars in the microscopic agglutination test (MAT) were divided in three groups: Group A (n=08): received two vaccine A doses with 30 days interval, Group B (n=08) two vaccine B doses with 30 days interval and Group C (n=08): control no vaccinated against leptospirosis.Blood samples were collected each 30 days during six months following the first vaccination. The sera were tested by MAT and growth inhibition test (GIT) to serovar Hardjo in order to evaluate respectively agglutinating and neutralizing antibodies. It was found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Leptospirosis; Immunity; Bacterines; Vaccination.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300013
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Comparison of four antibiotics for inactivating leptospires in bull semen diluted in egg yolk extender and experimentally inoculated with Leptospira santarosai serovar guaricura BJM
Miraglia,Fabiana; Morais,Zenaide Maria; Cortez,Adriana; Melville,Priscilla Anne; Marvullo,Maria Fernanda Vianna; Richtzenhain,Leonardo José; Visintin,José Antonio; Vasconcellos,Silvio Arruda.
Inactivation of leptospires in pools of semen from three Holstein Friesian bulls, collected in an artificial vagina, was investigated. Spermatic concentration was adjusted in egg yolk citrate extender, submitted to the following treatments: A (control; without antibiotics); B (penicillin, 1,000 UI/mL - streptomycin, 1,000 µg/mL); C (amoxicillin, 1,000 µg/mL); D (ceptiofur sodium, 1,000 µg/mL); E (amoxicillin 1,000 µg/mL - ceptiofur sodium 1,000 µg/mL). Leptospires (2.0 x 10(6) leptospires/mL) were added into the diluted semen. Recovery of leptospires was obtained in modified EMJH semi-solid medium with and without antibiotics. The antibiotics in the concentrations used did not affect means of percentage of progressive motility and individual progressive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospires; Semen; Antibiotics; Extender.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200011
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Cross neutralizing antibodies in hamsters vaccinated with leptospiral bacterins produced with three serovars of serogroup Sejroe BJM
Tabata,Rosana; Scanavini Neto,Hugo; Zuanaze,Marcelo Alexandre Fagnani; Oliveira,Eugenia Márcia de Deus; Dias,Ricardo Augusto; Morais,Zenaide Maria de; Ito,Fumio Honma; Vasconcellos,Silvio Arruda.
Three leptospiral bacterins, produced with different serovars of Serogroup Sejroe, namely the hardjo (bacterin A), wolffi (bacterin B) and guaricura (bacterin C), were evaluated in male hamsters (Mesocricetus auratus) by comparing the agglutinating and neutralizing antibodies titers using microscopic agglutination (MAT) and in vitro growth inhibition (GIT) tests. The immunization schedule was based on two 1.0 mL doses of non-diluted formalininactivated whole culture bacterin given through subcutaneous route with 10-day interval. The challenge was performed ten days after the second vaccine dose, when the animals were inoculated with 0.2 mL of non-inactivated cultures of each serovar through intraperitoneal route. On the 21st post-challenge day (PCD), all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Bacterins; Hamsters; Neutralizing antibodies; Serogroup Sejroe.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300016
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Cross-protection between experimental anti-leptospirosis bacterins BJM
Dib,Cristina Corsi; Gonçales,Amane Paldês; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Miraglia,Fabiana; Abreu,Patricia Antonia Estima; Vasconcellos,Silvio Arruda.
We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pomona; Hamsters; Bacterin; Cross-protection.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300042
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EMJH medium with 5-fluorouracil and nalidixic acid associated with serial dilution technique used to recover Leptospira spp from experimentally contaminated bovine semen BJM
Miraglia,Fabiana; Moraes,Zenaide Maria de; Melville,Priscilla Anne; Dias,Ricardo Augusto; Vasconcellos,Silvio Arruda.
Bovine semen experimentally contaminated with Leptospira santarosai serovar Guaricura was submitted to the modified EMJH medium with 5-fluorouracil (300mg/L) and nalidixic acid (20mg/L), named as "selective medium" and using the serial dilution technique, in order to evaluate the percentage of recovery of the added microorganism. The selective EMJH medium was found with higher percentage of recovery of leptospiras and minor losses of samples due to contamination with opportunistic microorganisms than the non-selective EMJH medium: 151/376 (40.0%) of positive growth; and 38/376 (10.0%) contamination and 58/376 (15%) and 129/376 (34.0%), respectively. These results were statistically significant (p<0. 0001; Fisher). Differences were found when the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospires; Culture media; Antimicrobial; Isolation; Semen.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100033
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Epidemiologia da Leptospirose em animais silvestres na Fundação Parque Zoológico de São Paulo Braz. J. Vet. Res. Anim. Sci.
Corrêa,Sandra Helena Ramiro; Vasconcellos,Silvio Arruda; Morais,Zenaide; Teixeira,Antoninho de Assis; Dias,Ricardo Augusto; Guimarães,Marcelo Alcindo de Barros Vaz; Ferreira,Fernando; Ferreira Neto,José Soares.
A Leptospirose é uma doença bacteriana de caráter zoonótico que afeta os animais domésticos, silvestres e o homem. Levantamentos sorológicos têm demonstrado o envolvimento de diferentes espécies sinantrópicas e silvestres na epidemiologia da doença. Com o objetivo de conhecer melhor a epidemiologia da Leptospirose dentro da Fundação Parque Zoológico de São Paulo, foi realizado um estudo sorológico nos animais silvestres mantidos em cativeiro, no período de 1996 a 1999. Foram colhidas amostras de sangue de 302 animais atendidos na rotina da Divisão de Veterinária, das quais 59 apresentaram resultado positivo (19.5%) para a prova de Soroaglutinação Microscópica. Os sorovares mais prováveis para o conjunto total de resultados foram : copenhageni...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal silvestre; Cativeiro; Zoológico; Leptospirose; Zoonose.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000300007
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Evaluation of the counterimmunoelectrophoresis reaction as a serodiagnosis test for swine leptospirosis BJM
Genovez,Margareth Élide; Yasuda,Paulo Hideki; Vasconcellos,Silvio Arruda; Scarcelli,Eliana; Cardoso,Maristela Vasconcellos; Girio,Raul da Silva.
The Counterimmunoelectrophoresis reaction (CIE) was tested as a genus specific test for swine leptospirosis diagnosis using three soluble extracts obtained from Leptospira sp, serovars pomona, icterohaemorrhagiae and patoc. The extracts were treated with hot Triton X-100 and applied to serum samples of animals divided in three groups: Group 1, 10 swines experimentally infected with the Pomona strain; Group 2, 50 naturally infected swines and Group 3, 10 swines control. These animals were serologically evaluated by CIE and by the Microscopic Agglutination Test (MAT), the WHO reference technique. Groups 1 and 3 were monitored during a period of 93 days after infection (a.i.). Group 1 serum convertion took place around the 10th day a.i. by MAT but ocurred...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Counterimmunoelectrophoresis; Swine leptospirosis; Seradiagnosis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200016
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Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry BJM
Karcher,Daniel; Grenfell,Rafaella C.; Moreno,Andrea Micke; Moreno,Luisa Zanolli; Vasconcellos,Silvio Arruda; Heinemann,Marcos B.; Almeida Junior,Joao N. de; Juliano,Luiz; Juliano,Maria A..
ABSTRACT Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Brazil; Identification; Mass spectrometry; MALDI-TOF.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400900
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Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil BJM
Miraglia,Fabiana; Moreno,Andréa Mike; Gomes,Cleise Ribeiro; Paixão,Renata; Liuson,Esequiel; Morais,Zenaide Maria; Maiorka,Paulo; Seixas,Fabiana Kömmling; Dellagostin,Odir Antonio; Vasconcellos,Silvio Arruda.
With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pomona; Swine; VNTR; Culture; PCR; Genital Tract.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017
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Isolation of leptospira Serovars Canicola and Copenhageni from cattle urine in the state of Paraná, Brazil BJM
Zacarias,Francielle Gibson da Silva; Vasconcellos,Silvio Arruda; Anzai,Eleine Kuroki; Giraldi,Nilson; Freitas,Julio Cesar de; Hartskeerl,Rudy.
In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were similar to Canicola (LO-14) and Copenhageni (LO-10). Microscopic agglutination test results show that Hardjo is the most common serovar in cattle in Brazil. Rats and dogs are the common maintenance hosts of serovars Copenhageni and Canicola. The excretion of highly pathogenic serovars such as Copenhageni and Canicola by cattle can represent an increasing risk for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Culture; Leptospirosis; Diagnosis; Cattle.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400028
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Leptospires detection in kidney, liver and uterus of cows slaughtered in Paraná State, Brazil BJM
Silva,Francielle Gibson da; Freitas,Julio Cesar de; Anzai,Eleine Kuroki; Hashimoto,Vanessa Yumi; Giraldi,Nilson; Delbem,Ádina Cléia B.; Bracarense,Ana Paula F.R.L.; Reis,Antônio Carlos F. dos; Vasconcellos,Silvio Arruda.
The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14), from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct examination detected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospire; Indirect immunoperoxidase; Immunohistochemistry; Cows; Culture.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100008
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Ocorrência de anticorpos anti-Brucella abortus e anti-Brucella canis em cães rurais e urbanos do Município de Monte Negro, Rondônia, Brasil Ciência Rural
Aguiar,Daniel Moura de; Cavalcante,Guacyara Tenório; Vasconcellos,Silvio Arruda; Megid,Jane; Salgado,Vanessa Riesz; Cruz,Tais Fukuta; Labruna,Marcelo Bahia; Pinter,Adriano; Silva,Jean Carlos Ramos da; Moraes,Zenaide Maria; Camargo,Luís Marcelo Aranha; Gennari,Solange Maria.
Foram avaliados 304 cães de ambiente rural e urbano do município de Monte Negro, Rondônia, através do Antígeno Acidificado Tamponado (AAT), Soroaglutinação Lenta em Tubos (SAL) e 2-Mercaptoetanol (2-ME) para a pesquisa de anticorpos anti-Brucella abortus e da Imunodifusão em gel de ágar (IDGA) e Imunodifusão em gel de ágar com soro tratado com 2-Mercaptoetanol (IDGA-ME) para Brucella canis. Foram consideradas positivas as amostras reagentes nas provas confirmatórias do 2-ME e IDGA-ME. Verificaram-se 56 (18,4%) animais reagentes ao AAT e 12 (4,0%) reagentes a SAL. Apenas um cão (0,3%) foi considerado positivo, confirmado pela prova do 2-ME. Foram observadas 11 (3,6%) reações á IDGA, porém não houve confirmação na prova do IDGA-ME. Ressalta-se a baixa...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brucella abortus; Brucella canis; Cães; Amazônia; Sorologia.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000500039
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Sorovares de leptospiras predominantes em exames sorológicos de bubalinos, ovinos, caprinos, eqüinos, suínos e cães de diversos estados brasileiros Ciência Rural
Favero,Andrea Cecília Mercaldi; Pinheiro,Sônia Regina; Vasconcellos,Silvio Arruda; Morais,Zenáide Maria; Ferreira,Fernando; Ferreira Neto,José Soares.
Em estudo retrospectivo abrangendo os anos de 1984 a 1997, foram realizados 15.558 exames sorológicos para leptospirose (SAM, com coleção de 24 sorovares), que incluíram: 284 ovinos, 879 bubalinos, 983 cães, 1.941 caprinos, 2.903 eqüinos e 8.568 suínos, distribuídos percentualmente por estado da seguinte forma: ovinos - SP (100%); bubalinos - SP (100%); cães - SP (80,7%), RS (0,10%), SC (0,10%) e PI (19,0%); caprinos - SP (33,1%), PB (63,7%) e CE (3,2%); eqüinos - SP (79,3%), RS (9,98%), SC (0,62%), PR (2,5%), RJ (0,17%), MG (1,96%), MT (3,99%), PB (1,3%) e PI (0,03%); suínos - SP (61,91%), RS (0,3%), SC (5,95%), PR (3,67%), RJ (0,88%), MG (24,38%), GO (1,12%), SE (0,2%), PE (0,90%), CE (0,34%) e MA (0,1%). A distribuição temporal dos animais examinados...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorologia; Aglutinação; Leptospirose; Bubalinos; Ovinos; Caprinos; Eqüinos; Suínos; Cães.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000400011
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Teste de tuberculinização em caprinos (Capra hircus) experimentalmente sensibilizados Ciência Rural
Silva,Paulo Eduardo Gomes da; Pinheiro,Sônia Regina; Leal,Marta Lizandra do Rego; Bertagnon,Heloísa Godoi; Motta,Pedro Moacyr Pinto Coelho; Sinhorini,Idércio Luiz; Vasconcellos,Silvio Arruda; Benesi,Fernando José.
Foram estabelecidos critérios de interpretação do teste de tuberculinização aplicado ao diagnóstico da tuberculose em grupos de caprinos experimentalmente sensibilizados. Dos 30 animais utilizados, dez foram sensibilizados com Mycobacterium avium - amostra D4 (grupo A) e dez com Mycobacterium bovis - amostra AN5 (grupo B). Dez caprinos não foram sensibilizados e constituíram o grupo controle (grupo C). No teste cervical simples, realizado com tuberculina bovina (M. bovis) e lido às 72 horas pós-tuberculinização (p.t.), as reações positivas foram aquelas em que houve aumento da espessura da dobra de pele igual ou superior a 3,9mm; reações inconclusivas, quando situadas entre 1,8 e 3,8mm, e negativas quando iguais ou menores que 1,7mm. A análise dos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculose; Teste de tuberculina; Caprinos.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000300023
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Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis BJM
Rosário,Tatiana Reis do; Dib,Cristina Corsi; Roxo,Eliana; Pinheiro,Sônia Regina; Vasconcellos,Silvio Arruda; Benites,Nilson Roberti.
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Mycobacterium bovis; Thin layer; PCR; Cattle and buffalo organs.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100031
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