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Inflammatory response in a rat model of gastroschisis is associated with an increase of NF-kappaB BJMBR
Sbragia,L.; Schmidt,A.F.; Moraes,S.; Bittencourt,D.G.; Gonçalves,F.L.L.; Pereira,L.A.V.D.; Velloso,L.A..
Babies with gastroschisis have high morbidity, which is associated with inflammatory bowel injury caused by exposure to amniotic fluid. The objective of this study was to identify components of the inflammatory response in the intestine and liver in an experimental model of gastroschisis in rats. The model was surgically created at 18.5 days of gestation. The fetuses were exposed through a hysterotomy and an incision at the right of the umbilicus was made, exposing the fetal bowel. Then, the fetus was placed back into the uterus until term. The bowel in this model had macro- and microscopic characteristics similar to those observed in gastroschisis. The study was conducted on three groups of 20 fetuses each: gastroschisis, control, and sham fetuses. Fetal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gastroschisis; Inflammation; Inflammatory mediators; Nuclear factor kappaB.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200006
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Insulin receptor has tyrosine kinase activity toward Shc in rat liver BJMBR
Páez-Espinosa,E.V.; Carvalho,C.R.O.; Velloso,L.A.; Saad,M.J.A..
Insulin induces tyrosine phosphorylation of Shc in cell cultures and in insulin-sensitive tissues of the intact rat. However, the ability of insulin receptor (IR) tyrosine kinase to phosphorylate Shc has not been previously demonstrated. In the present study, we investigated insulin-induced IR tyrosine kinase activity towards Shc. Insulin receptor was immunoprecipitated from liver extracts, before and after a very low dose of insulin into the portal vein, and incubated with immunopurified Shc from liver of untreated rats. The kinase assay was performed in vitro in the presence of exogenous ATP and the phosphorylation level was quantified by immunoblotting with antiphosphotyrosine antibody. The results demonstrate that Shc interacted with insulin receptor...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Tyrosine kinase activity; Insulin receptor; Shc; Insulin action.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100008
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