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Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk BABT
Kaur,Hardeep; Kumar,Sachin; Verma,Neelam.
The aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples. Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6µm was achieved in the milk and the enzyme membranes were stable for more than two months at 4ºC. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 µm. Lead specificity was attained by means of masking agents 1,10 -...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosensor; Urease; Hydrosol- gel immobilization; Lead; Milk; Masking agent.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400613
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Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol BABT
Singh,Jagdish; Verma,Neelam.
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Glucose oxidase; Aqueous two phase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007
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Screening of efficient arbuscular mycorrhizal fungi for Azadirachta indica under nursery condition: a step towards afforestation of semi-arid region of western India BJM
Banerjee,K.; Gadani,M.H.; Srivastava,K.K.; Verma,Neelam; Jasrai,Y.T.; Jain,N.K..
To optimize nursery practices for efficient plant production procedures and to keep up to the ever growing demand of seedlings, identification of the most suitable species of arbuscular mycorrhizal fungi (AMF), specific for a given tree species, is clearly a necessary task. Sixty days old seedlings of Neem (Azadirachta indica A. Juss) raised in root trainers were inoculated with six species of AMF and a mixed inoculum (consortia) and kept in green house. Performances of the treatments on this tree species were evaluated in terms of growth parameters like plant height shoot collar diameter, biomass and phosphorous uptake capabilities. Significant and varied increase in the growth parameters and phosphorous uptake was observed for most of the AMF species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arbuscular mycorrhizal fungi; Neem; Glomus mosseae; Glomus intraradices.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200039
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