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A shortcut method for sexing juvenile European sea bass, Dicentrarchus labrax L. ArchiMer
Menu, Bruno; Peruzzi, Stefano; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice.
The objective of this study was to develop a rapid and effective method of sexing juvenile sea bass with minimum labour and material. To this end, the gonad squash mount technique was applied along with macroscopic techniques for sexing a large number of experimental fish at the age of 215-275 days post fertilization (p.f.). At this age, 90% of the 3894 fish could be unambiguously sexed by macroscopic examination of their gonads, whereas the remaining proportion was identified using squash mount preparations (10%). The accuracy of the observations was measured up to classical histology procedures. Undifferentiated fish accounted for 0.1% of the total population only. The application of the squash mount technique holds out the prospect of considerable...
Tipo: Text Palavras-chave: Histology; Squash; Juvenile; Gonads; Seabass; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-378.pdf
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Early individual electronic identification of sea bass using RFID microtags: A first example of early phenotyping of sex-related growth ArchiMer
Ferrari, Sebastien; Chatain, Beatrice; Cousin, Xavier; Leguay, Didier; Vergnet, Alain; Vidal, Marie-odile; Vandeputte, Marc; Begout, Marie-laure.
Although individual electronic tagging using PIT (Passive Integrated Transponder) tags is well established, it is mainly used for fish > 60 mm in length. Since electronic tagging is an ideal identification method, we used RFID (Radio Frequency Identification) microtags (6 mm in length, 1 mm in diameter, 10 mg in mass) to characterize individual fish from the early stages of their development and throughout their lifecycles. We used sea bass, Dicentrarchus labrax, (105 day-old fish weighing between 100 and 1100 mg) and studied the effects of intra-coelomic tagging in half the population using different endpoints including survival and tag reading, growth over 6 months and swimming responses. Dead fish were counted daily, biometric data were collected at...
Tipo: Text Palavras-chave: Tagging effects; Swimming behavior; Sexual dimorphism; Dicentrarchus labrax.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00175/28645/27072.pdf
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Estimation of body weight of European sea bass (Dicentrarchus labrax) and Nile tilapia (Oreochromis niloticus) larvae by image analysis ArchiMer
De Verdal, Hugues; Vandeputte, Marc; Pepey, Elodie; Vidal, Marie-odile; Ouedraogo, C; Canonne, Marc; D'Cotta, Helena; Baroiller, Jean Francois; Baras, E; Chatain, Beatrice.
The present study aimed to develop nondestructive methods to accurately follow the growth of fish larvae and post-larvae. European sea bass (Dicentrarchus labrax) aged 71-100 days post fertilization (dpf; 20-419 mg) and Nile tilapia (Oreochromis niloticus) aged 14 dpf (10-25 mg) were used as model species. Several measurements were performed on digital pictures of larvae to model body weight. Using body surface area, contour, length and depth, it was possible to estimate accurately the body weight of young European sea bass and Nile tilapia (r² of 0.98 and 0.95, respectively).
Tipo: Text Palavras-chave: Image analysis; Body weight; Surface area; European sea bass; Nile tilapia.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00205/31619/30045.pdf
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Genetic variation for carcass quality traits in cultured sea bass (Dicentrarchus labrax) ArchiMer
Saillant, Eric; Dupont-nivet, Mathilde; Sabourault, Marie; Haffray, Pierrick; Laureau, Stanislas; Vidal, Marie-odile; Chatain, Beatrice.
Genetic parameters for carcass quality traits were estimated in 27 families of sea bass (3 dams x 9 sires factorial mating design), raised mixed in the same tanks starting before hatching. O. spring parentage was determined a posteriori using 6 microsatellite loci. Carcass quality traits were recorded at 818 days post fertilization (mean standard length: 32.6 +/- 3.1 cm). Genetic parameters were estimated from the sire half sib variance and covariance components. Heritability of body weight (BW) and carcass processing traits (standardized to body weight) percent head weight (Head%), percent viscera weight (Viscera%) and percent visceral fat weight (VisceFat%) were relatively high ranging from 0.48 +/- 0.15 (Viscera%) to 0.87 +/- 0.23 (Head%); the estimate...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Communal testing; Microsatellites markers; Carcass quality; Heritability.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6355.pdf
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Individual growth monitoring of European sea bass larvae by image analysis and microsatellite genotyping ArchiMer
De Verdal, Hugues; Vandeputte, Marc; Pepey, Elodie; Vidal, Marie-odile; Chatain, Beatrice.
The aims of the present study were to develop non-lethal methods to identify individual fish larvae and post-larvae before tagging and accurately follow their growth characteristics. European sea bass (Dicentrarchus labrax) was used as a model species at four different ages ranging from 71 to 100 days post fertilization (dpf). Two different methods were tested for non-lethal tissue sampling from each larva for DNA analysis: 1) using a sterile absorbent paper to sample mucus and/or epithelial cells by rubbing the fish skin and 2) fin-clip of the bottom part of the caudal fin. Whatever the age of the larvae, the genotyping rate (at 12 microsatellite markers) was low with the use of sterile absorbent paper but relatively high with fin-clip sampling at 80 and...
Tipo: Text Palavras-chave: Individual identification; Image analysis; Body weight; Surface area; Perimeter; European sea bass larvae.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00211/32195/30748.pdf
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Probiotic treatment of live food organisms for Atlantic bluefin tuna larvae: microbiological & immunological criteria ArchiMer
Coves, Denis; De Vogue, Benoist; Desbruyeres, Elisabeth; Dhormes, Benoit; Fievet, Julie; Huelvan, Christine; Lallement, Stephane; Le Gall, Marie-madeleine; Ruelle, Francois; Vidal, Marie-odile; Castex, M.; Mazurais, David; Cahu, Chantal; Gatesoupe, François-joel.
Tipo: Text
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00051/16189/13702.pdf
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Response to domestication and selection for growth in the European sea bass (Dicentrarchus labrax) in separate and mixed tanks ArchiMer
Vandeputte, Marc; Dupont Nivet, M; Haffray, P; Chavanne, H; Cenadelli, S; Parati, K; Vidal, Marie-odile; Vergnet, Alain; Chatain, Beatrice.
Selective breeding of European sea bass (Dicentrarchus labrax) receives a growing interest, as the estimated heritability of growth is medium to high. In this study, we compared the offspring of four groups of sea bass sires, mated with the same wild dams: wild (W), first generation of domestication (D), first generation of mass selection for length (M), first generation of PROSPER-like selection for length (P). The comparison was done both in replicated tanks (separate rearing) and in mixed tanks (mixed rearing) where sire origins were recovered by genotyping of eight microsatellite markers. Weight, length and growth rate were measured from day 238 post-fertilization (69 g mean weight) to day 611 post-fertilization (390 g mean weight). Both in mixed and...
Tipo: Text Palavras-chave: Nephrocalcinosis; Competition; Microsatellites; Parentage assignment; Realized heritability; Selection response; Growth; Selective breeding; Dicentrarchus labrax; European sea bass.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-5303.pdf
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Transcriptomic Profiling of Egg Quality in Sea Bass (Dicentrarchus labrax) Sheds Light on Genes Involved in Ubiquitination and Translation ArchiMer
Zarski, Daniel; Thaovi Nguyen,; Le Cam, Aurelie; Montfort, Jerome; Dutto, Gilbert; Vidal, Marie-odile; Fauvel, Christian; Bobe, Julien.
Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed. An Agilent oligo microarray experiment was performed on labelled mRNA extracted from 16 batches of eggs (each batch obtained from a different female) of sea bass, in which over 24,000 published probe arrays were...
Tipo: Text Palavras-chave: Microarray; Genomics; Transcriptomics; Aquaculture; Controlled reproduction.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48220/48345.pdf
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Unpredictability in food supply during early life influences growth and boldness in European Seabass, Dicentrarchus labrax ArchiMer
Ferrari, Sebastien; Leguay, Didier; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice; Begout, Marie-laure.
Biological variability is no longer considered as statistical noise, but rather as an adaptive benefit. This variability comes from consistent differences in behavioral and physiological responses among individuals to a changing/challenging environment, named “coping style”, “temperament” or “personality”. Many studies have described how to characterize personality traits and how to assess their consistency over time and between different contexts; however, little is known about the environmental factors shaping personality development. Because contrasting personalities are maintained with evolution, this lead to the widespread assumption that genes play a predominant role in personality. In many cases, personality traits are however also likely to be...
Tipo: Text
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00333/44433/44100.pdf
Registros recuperados: 9
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