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Chatel, Amelie; Faucet-marquis, Virginie; Perret, Marine; Gourlay-france, Catherine; Uher, Emmanuelle; Pfohl-leszkowicz, Annie; Vincent-hubert, Francoise. |
The use of DNA adduct analysis has previously focused on the use of marine organisms for biomonitoring, whereas similar investigations in freshwater organisms are sparse. In that context, we have investigated the relevance of DNA adducts as biomarkers of genotoxicity in the freshwater mussels Dreissena polymorpha. The objective of the present study is to determine the stability of DNA adducts induced by benzo[a]pyrene (B[a]P) in zebra mussels. Mussels were exposed to dissolved B[a]P (10100 mu g/l) for 4 days. Afterwards, mussels were kept in clean water for 28 days and DNA adduct levels were subsequently measured in two different organs, the digestive glands and the gills, using the P-32-postlabelling technique. In parallel, the expression of genes... |
Tipo: Text |
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Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00184/29566/27912.pdf |
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Gourlay France, Catherine; Vincent-hubert, Francoise; Tusseau-vuillemin, Marie-helene; Sanchez, Wilfried; Geffard, Alain; Levi, Yves; Oziol, Lucie; Labadie, Pierre; Mouchel, Jean-marie; Raguet, Mireille; Thery, Sylvain. |
Le PIREN-Seine s’intéresse à la présence dans le bassin versant de la Seine de contaminants d’origines agricole, industrielle et domestique depuis une vingtaine d’années. Ces polluants, qui se retrouvent parfois à des concentrations très faibles dans l’eau, peuvent avoir des impacts sur l’homme et l’environnement qui restent peu connus. La présence de ces substances toxiques suscite un intérêt majeur auprès des gestionnaires de la ressource comme des consommateurs. Les perturbations endocriniennes, les effets cancérigènes, la présence de résidus de médicaments sont autant de sujets d’inquiétude largement médiatisés et débattus. Pour garantir la protection de la santé humaine et du milieu aquatique, la réglementation a défini des normes de qualité... |
Tipo: Text |
Palavras-chave: POLLUTION DE L'EAU; PCB; DIOXINE; PRODUIT PHYTOSANITAIRE; ORGANOCHLORE; ECOTOXICOLOGIE. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00185/29580/27924.pdf |
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Vincent-hubert, Francoise; Chatel, Amelie; Gourlay-france, Catherine. |
We have previously shown that cadmium (Cd) and Benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), Aryl Hydrocarbon Receptor (AHR), P-gP, catalase, glutathione S-transferase and Heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gP and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase... |
Tipo: Text |
Palavras-chave: Benzo[a]pyrene; Cadmium; Metallothionein; Aryl hydrocarbon receptor; Antioxidant enzymes; RT-qPCR. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29547/27873.pdf |
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