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DNA barcoding in Mucorales: an inventory of biodiversity Naturalis
Walther, G.; Pawłowska, J.; Alastruey-Izquierdo, A.; Wrzosek, M.; Rodriguez-Tudela, J.L.; Dolatabadi, S.; Chakrabarti, A.; Hoog, G.S. de.
The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and...
Tipo: Article / Letter to the editor Palavras-chave: Backusella; Biodiversity; Clinical relevance; DNA barcoding; Intraspecific variability; ITS; LSU; Mucor; Mucorales; Nomenclature; Rhizomucor; Taxonomy; Zygorhynchus.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/532353
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The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies Naturalis
Hoffmann, K.; Pawłowska, J.; Walther, G.; Wrzosek, M.; Hoog, G.S. de; Benny, G.L.; Kirk, P.M.; Voigt, K..
The Mucorales (Mucoromycotina) are one of the most ancient groups of fungi comprising ubiquitous, mostly saprotrophic organisms. The first comprehensive molecular studies 11 yr ago revealed the traditional classification scheme, mainly based on morphology, as highly artificial. Since then only single clades have been investigated in detail but a robust classification of the higher levels based on DNA data has not been published yet. Therefore we provide a classification based on a phylogenetic analysis of four molecular markers including the large and the small subunit of the ribosomal DNA, the partial actin gene and the partial gene for the translation elongation factor 1-alpha. The dataset comprises 201 isolates in 103 species and represents about one...
Tipo: Article / Letter to the editor Palavras-chave: Mucorales; Families; Phylogeny.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/532035
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