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Bacillus Coagulans Enhance the Immune Function of the Intestinal Mucosa of Yellow Broilers 89
Xu,L; Fan,Q; Zhuang,Y; Wang,Q; Gao,Y; Wang,C.
ABSTRACT This experiment was conducted to investigate the effects of Bacillus coagulans on the growth performance and immune functions of the intestinal mucosa of yellow broilers. Three hundred and sixty one-day-old yellow chicks were randomly allocated to four treatments groups with six replicates of 15 chicks each. The broilers were randomly subjected to one of the following treatments for 28 days: control group (group1, fed a basal diet) and three treatments (group 2, 3, 4) fed the basal diet supplemented with 100, 200, or 300 mg/kg Bacillus coagulans , respectively). The results showed that for 28 days, compared with the control diet, the dietary addition of 200 mg/kg Bacillus coagulans significantly decreased the feed/gain ratio (F/G) (p<0.05),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus coagulans; Yellow broilers; Growth performance; Intestinal mucosa immune function..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100115
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Comparison of isozyme transformation in maize as a result of insertion of the chitinase gene 85
Yan,PM; Zhang,HF; Wang,Q; Yan,XY; Sun,Y.
Isozymes of peroxidase (POD), catalase (CAT), esterase (EST) and superoxide dismutase (SOD) were analyzed on transgenic maize (with external chitinase gene) and its parent by vertical polyacrylamide gel electrophoresis (PAGE). This study was made using shoots at the fourth leaf stage. Results showed that: POD and EST were detected in 6 bands. POD-2 and POD-3 were present at the bud and seedling stages. POD-1, POD-4, POD-5 and POD-6 were only present at the seedling stage. POD-6 expressed stronger in the transgenic maize with chitinase than in its parent. EST-2 was present only at the bud stage, and its expression in transgenic maize was stronger than that in its parent. EST-5 only existed at the seedling stage. EST-4 did not exist in the parent maize...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Isozyme; Maize; Antioxidant enzyme system.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200002
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Distribution of Ifitm3 in Yellow-Feathered Broilers and Inhibition of Avian Reovirus Multiplication by Ifitm3 89
Wang,Q; Yuan,X; Li,C; Deng,H; Wang,C.
ABSTRACT This study was carried to express the interferon-induced transmembrane protein 3 (IFITM3) in vitro and examine its function in inhibition of avian reovirus (ARV) replication. The recombinant prokaryotic vector expressing yellow-feathered broiler IFITM3 was successfully constructed, and the recombinant protein was expressed in competent Escherichia coli BL21 cells. New Zealand white rabbits were immunized with the purified recombinant protein to prepare a polyclonal antibody, with a titer of 1:128,000. Immunohistochemistry, reverse transcription-PCR, and real-time fluorescence quantitative PCR showed that IFITM3 was distributed in the yellow-feathered broiler immune organs, and the expression of IFITM3 in bursa of Fabricius was more than in spleen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yellow-feathered broiler; Interferon; Interferon-induced transmembrane protein 3; Gene cloning; Prokaryotic expression; Avian Reovirus.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200377
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Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle 89
Gao,G; Wang,H; Zhao,X; Li,Q; Wang,C; Li,J; Zhang,K; Zhong,H; Wang,Q.
ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feeding conditions; Chicken; FATP1; Meat quality; Intramuscular fat.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055
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Effects of Dietary Energy Concentration on Reproductive Hormone Secretion and Gene Expression in the Hypothalamus-Pituitary-Gonad Axis in Laying Geese 89
Liu,Z; Xue,J; Luo,Y; Wang,Q; Zhong,H; Liang,M; Wang,C.
ABSTRACT The objective of the study was to investigate the mechanism by which dietary energy concentration regulates laying performance in geese. Eighty 558-day-old female Sichuan White geese were randomly allotted to two dietary treatments, each treatment was fed 1 of 2 experimental diets containing 10.00 (deficient) or 11.80MJ/kg metabolizable energy (sufficient) for 30 days. Laying performance, hormone concentration and gene expressions in hypothalamus-pituitary-gonadal axis were examined in geese. Birds fed the sufficient-energy diet had significantly higher average egg weight, daily laying rate, and lower feed to egg ratio than those fed the deficient-energy (p<0.05). The birds fed sufficient-energy diet had higher concentration of serum insulin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Energy concentration; Laying geese; Reproductive hormone; Gene expression.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300306
Registros recuperados: 5
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