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| Gao,Zhengquan; Meng,Chunxiao; Gao,Hongzheng; Zhang,Xiaowen; Xu,Dong; Su,Yuanfeng; Wang,Yuanyuan; Zhao,Yuren; Ye,Naihao. |
| The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on... |
| Tipo: Journal article |
Palavras-chave: Haematococcus pluvialis astaxanthin 2; 4-Epibrassinolide (EBR) carotenoid genes real-time fluorescence quantitative PCR (qRT-PCR). |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200012 |
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| Wei,Shiping; Hurley,James; Jiang,Zhenglong; Wang,Siwen; Wang,Yuanyuan. |
| The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Riboflavin; Ashbya gossypii; Ultraviolet irradiation; Medium optimization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200003 |
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