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A PCR-based survey of animal African trypanosomosis and selected piroplasm parasites of cattle and goats in Zambia OAK
Musinguzi, Simon Peter; Suganuma, Keisuke; Asada, Masahito; Laohasinnarong, Dusit; Sivakumar, Thillaiampalam; Yokoyama, Naoaki; Namangala, Boniface; Sugimoto, Chihiro; Suzuki, Yasuhiko; Xuan, Xuenan; Inoue, Noboru.
We screened cattle and goats from the districts of Chama, Monze and Mumbwa in Zambia for animal African trypanosomes, Babesia bigemina and Theileria parva using PCRs; 38.1% of the samples tested positive for at least one of the parasite species. The most common parasite was Trypanosoma vivax (19.8%). Its incidence was significantly higher in goats than in cattle, (P<0.05). B. bigemina was found in samples from all the three areas, making it the most widespread of the parasites in Zambia. Among the tested samples, 12.0% of the positive samples were mixed infections. There were significant differences in the infection rates of T. vivax (Mumbwa had a significantly higher infection rate [39.6%, P<0.0001]), Th. parva (Monze had the only cases...
Palavras-chave: Animal African trypanosomosis; Cattle; Goat; Piroplasmosis; Zambia.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4389
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Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites OAK
Zhang, Houshuang; Compaore, Muller K.A.; Lee, Eung-goo; Liao, Min; Zhang, Guohong; Sugimoto, Chihiro; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan.
The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Apical membrane antigen 1; Cross-reactive; Invasion.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1034
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Artesunate, a potential drug for treatment of Babesia infection OAK
Goo, Youn-Kyoung; Terkawi, M. Alaa; Jia, Honglin; Aboge, G. Oluga; Ooka, Hideo; Kim, Suk; Igarashi, Ikuo; Nishikawa, Yoshifumi; Xuan, Xuenan.
The effect of artesunate, a water-soluble artemisinin derivative, against Babesia species, such as Babesia bovis, Babesia gibsoni, and Babesia microti was studied. Cultures of B. bovis and B. gibsoni were treated with 0.26 μM, 2.6 μM, 26 μM, and 260 μM artesunate, and the growth-inhibitory effect was shown in over 2.6 μM artesunate in day 4 and day 3 post-subculture for B. bovis and B. gibsoni, respectively, in dose-dependent manner. In vivo experiment for B. microti, strong inhibition effects were observed in mouse groups treated with over 1.0 mg/kg body weight of artesunate on day 9 and 10 post-infection. These results suggest that artesunate could be a potential drug for Babesia infection.
Palavras-chave: Artesunate; Babesia bovis; Babesia gibsoni; Babesia microti.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2820
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Babesia gibsoni: An apical membrane antigen-1 homologue and its antibody response in the infected dogs OAK
Zhou, Jinlin; Yang, Jun; Zhang, Guohong; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan.
A cDNA encoding the apical membrane antigen-1 (AMA-1) homologue was obtained by immunoscreening a cDNA expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 2062 bp. Computer analysis suggested that the sequence contains an open reading frame of 1794 bp with a coding capacity of approximately 66 kDa. Based on the homology analysis, this putative protein was designated as B. gibsoni AMA-1 (BgAMA-1). The BgAMA-1 gene was expressed in the Escherichia coli BL21 strain and used as the antigen in Western blotting and the enzyme-linked immunosorbent assay (ELISA). The results indicated that BgAMA-1 was recognized as an immunodominant antigen by the host immune system and that it induced a strong antibody...
Palavras-chave: Babesia gibsoni; AMA-1; Antibody response.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/815
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Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen OAK
Zhou, Jinlin; Jia, Honglin; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan.
A cDNA encoding the rhoptry-associated protein 1 (RAP-1) homologue was obtained by immunoscreening an expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1740bp. Computer analysis suggested that the sequence contains an open reading frame of 1425bp encoding an expected protein with a molecular weight of 52kDa. Based on the sequence similarity, this putative protein was designated as the B. gibsoni RAP-1 (BgRAP-1). The BgRAP-1 gene was expressed in the Escherichia coli BL21 strain, and the recombinant BgRAP-1 was used as the antigen in the enzyme-linked immunosorbent assay (ELISA). The results can differentiate between the B. gibsoni-infected dog sera and the Babesia canis infected dog sera or...
Palavras-chave: Babesia gibsoni; Rhoptry-associated protein; Enzyme-linked immunosorbent assay; Diagnosis.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/811
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Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B-microti infection in mice OAK
Terkawi, M. Alaa; Jia, Honglin; Zhou, Jinlin; Lee, Eung-goo; Igarashi, Ikuo; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan.
Babesia gibsoni ribosomal phosphoprotein PO (BgP0) was identified as an immunodominant cross-reactive antigen with B. microti. The BgPO gene is a single copy with a predicted open reading frame of 942 bp and 314 amino acids. The BgP0 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice with the recombinant BgPO showed a specific band with a 34-kDa molecular mass in the extracts of B. gibsoni and B. microti merozoites. Furthermore, the intraperitoneal (i.p.) immunization of rBgP0 and Freund's adjuvant induced strong Immoral response consisting of mixed immunoglobulins IgG1 and IgG2a in BALB/c mice. Following the challenge with B. microti, these mice delayed the onset of parasites and significantly reduced...
Palavras-chave: B. gibsoni; B. microti; Ribosomal phosphoprotein P0; Cross-reactive antigen.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1036
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Characterization of a leucine aminopeptidase from Toxoplasma gondii OAK
Jia, Honglin; Nishikawa, Yoshifumi; Luo, Yuzi; Yamagishi, Junya; Sugimoto, Chihiro; Xuan, Xuenan.
The M17 family leucine aminopeptidase (LAP) hydrolyze amino acids from the N-terminus of peptides. Many LAPs from parasitic protozoa including Plasmmodium, Trypanosoma and Leishmania, have been intensely investigated because of their crucial roles in parasite biology. In this study, a functional recombinant Toxoplasma gondii LAP (rTgLAP) was expressed in Escherichia coli and its enzymatic activity against synthetic substrates for aminopeptidase, as well as the cellular localization was determined. Our results indicated that TgLAP is a functional aminopeptidase in the cytoplasma of T. gondii.
Palavras-chave: Toxoplasma gondii; Leucine aminopeptidase; Enzymatic activity.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2822
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Characterization of a novel thrombospondin-related protein in Toxoplasma gondii OAK
Kawase, Osamu; Nishikawa, Yoshifumi; Bannai, Hiroshi; Matsuo, Tomohide; Xuan, Xuenan.
Toxoplasma gondii is an obligate intracellular protozoan parasite that invades a wide range of host cells. Upon encountering host cells, the parasite releases a large variety of proteins from secretory organelles, such as micronemes, rhoptries and dense granules. The secretion of microneme protein is essential for parasite invasion. We found that a secreted protein with an altered thrombospondin repeat of Toxoplasma gondii (TgSPATR) was a novel microneme protein, which was different from known microneme proteins that carry thrombospondin repeat domains. TgSPATR was secreted in response to an intra-parasitic elevation of Ca2+ and probably secreted during early stages of parasite invasion. Thus, we suggested that TgSPATR, new member of microneme secretory...
Palavras-chave: Ca2+-dependent secretion; Microneme protein; Secreted protein with an alteredthrombospondin repeat; Toxoplasma gondii.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2819
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Characterization of P15 Antigen of Cryptosporidium parvum Expressed by a Recombinant Vaccinia Virus OAK
Xuan, Xuenan; Zhang, Sofa; Kamio, Tsugihiko; Tsushima, Y.; Kamada, Takenori; Nishikawa, Yoshifumi; Otsuka, Haruki; Karanis, Panagiotis; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; 玄, 学南; 西川, 義文; 五十嵐, 郁男.
Palavras-chave: C. parvum; P15; Vaccinia virus; Subunit vaccine.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/314
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Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 OAK
Miyama, Takako; Inokuma, Hisashi; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; 猪熊, 壽; 玄, 学南.
The clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 was examined in dogs in an area where B. gibsoni infection was endemic. Only 8 among 14 dogs with acute type B. gibsoni infection without a previous history of infection were positive. This high percentage of false-negative results is thought to be a weak point of ELISA as a diagnostic tool. However, 14 other anemic dogs with a confirmed history of B. gibsoni infection were all positive, thus confirming the higher sensitivity of ELISA in detecting a history of infection.
Palavras-chave: Babesia gibsoni; Diagnosis; ELISA.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/925
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CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS OAK
Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624
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Construction of Recombinant Feline Herpesvirus Type 1 Expressing Toxoplasma gondii SRS1 OAK
Mishima, Masayuki; Xuan, Xuenan; Takeiri, Akira; Makala, Levi H. C; Igarashi, Ikuo; Fujisaki, Kozo; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Toxoplasma; Vaccine; SRS1; FHV.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/343
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Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23 OAK
Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男.
To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the...
Palavras-chave: Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/129
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CURRENT SERODIAGNOSTIC MEASURES FOR EQUINE BABESIOSIS OAK
Hirata, Haruyuki; Yokoyama, Naoaki; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; Babesia caballi; Diagnosis; ELISA; ICT.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/638
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Detection of Antibodies to Babesia equi by The Latex Agglutination Test with Recombinant Merozoite Antigen-2 OAK
Tanaka, Tetsuya; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; EMA-2; Baculovirus; Latex agglutination test.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/329
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Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle OAK
Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%)....
Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142
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Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction OAK
Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction(PCR)method.The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001%. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281
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DNA vaccine coding a p23 protein of Cryptosporidium parvum fused with Fc portion of immunogloblin G induces higher level of interferon-γ expression in mice OAK
Takashima, Yasuhiro; Xuan, Xuenan; Shirafuji, Hiroaki; Zhang, Guohong; Otsuka, Haruki.
To develop DNA vaccines against Cryptosporidiosis, a plasmid coding an immunodominant protein of Cryptosporidium parvum sporozoite, p23 (pCX-p23) and another plasmid coding a fusion protein containing whole the p23 and the Fc portion of mouse immunogloblin G1 (pCX-p23Fc). Vaccination of BALB/c mice with the plasmid, pCX-p23 and pCX-p23Fc induced the production of antibodies against p23. Although both of splenocytes of mice immunized with the plasmids pCX-p23 and pCX-p23Fc expressed interleukin-4 and interferon-8, after the in vitro stimulation by p23 antigen, the interferon-8 expression level of pCX-p23Fc immunized mice was much higher than that of pCX-p23 immunized mice. These results suggest a possibility of the plasmid pCX-p23Fc as a DNA vaccine...
Palavras-chave: C. parvum; DNA vaccine; Fc; Interferon-8.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/147
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Emergence of multi-acaricide resistant Rhipicephalus ticks and its implication on chemical tick control in Uganda OAK
Vudriko, Patric; Okwee-Acai, James; Tayebwa, Dickson Stuart; Byaruhanga, Joseph; Kakooza, Steven; Wampande, Edward; Omara, Robert; Muhindo, Jeanne Bukeka; Tweyongyere, Robert; Owiny, David Okello; Hatta, Takeshi; Tsuji, Naotoshi; Umemiya-Shirafuji, Rika; Xuan, Xuenan; Kanameda, Masaharu; Fujisaki, Kozo; Suzuki, Hiroshi.
Background: Acaricide failure has been on the rise in the western and central cattle corridor of Uganda. In this study, we identified the tick species associated with acaricide failure and determined their susceptibility to various acaricide molecules used for tick control in Uganda. Methods: In this cross sectional study, tick samples were collected and identified to species level from 54 purposively selected farms (from 17 districts) that mostly had a history of acaricide failure. Larval packet test was used to screen 31 tick populations from 30 farms for susceptibility at discriminating dose (DD) and 2 x DD of five panels of commercial acaricide molecules belonging to the following classes; amidine, synthetic pyrethroid (SP), organophosphate (OP) and...
Palavras-chave: Ticks; Rhipicephalus appendiculatus; Rhipicephalus (Boophilus) decoloratus; Acaricide; Resistance; Amitraz; Synthetic pyrethroids; Organophosphates.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4437
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Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey OAK
Zhou, Mo; Cao, Shinuo; Sevinc, Ferda; Sevinc, Mutlu; Ceylan, Onur; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Suzuki, Hiroshi; Nishikawa, Yoshifumi; Xuan, Xuenan.
Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T gondii and N. caninum infections are prevalent in Turkish domestic animals.
Palavras-chave: ELISA; Neospora caninum; Toxoplasma gondii; Turkey.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4422
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