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Characterization of a Gelatin/Chitosan/Hyaluronan scaffold-polymer 69
Enrione,Javier; Osorio,Fernando; López,Daniel; Weinstein-Oppenheimer,Caroline; Fuentes,Miguel A; Ceriani,Ricardo; Brown,Donald I; Albornoz,Fernando; Sánchez,Elizabeth; Villalobos,Patricio; Somoza,Rodrigo A; Young,Manuel E; Acevedo,Cristian A.
Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30ºC could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin,...
Tipo: Journal article Palavras-chave: Chitosan; Fibrin; Gelatin; Hyaluronic acid; Scaffold.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500020
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Measuring b-Galactosidase activity at pH 6 with a differential pH sensor 69
Acevedo,Cristian; Stach,Matthias H; Amtmann,Anette; Young,Manuel E; Reyes,Juan G; Huebner,Holger; Buchholz,Rainer.
The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.
Tipo: Journal article Palavras-chave: B-Galactosidase; Delta milli pH; Differential pH.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200012
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The application of polymerized lipid vesicles as colorimetric biosensors for real-time detection of pathogens in drinking water 69
Villalobos,Patricio; Chávez,María Isabel; Olguín,Yusser; Sánchez,Elizabeth; Valdés,Erika; Galindo,Raúl; Young,Manuel E.
The inadequate treatments given to the served waste water which are disposal to the rivers and sea coast are the major sources of faecal Microorganisms and enteric bacterial pathogens. They are among the most serious effects of water pollution bringing risks on public health. None of the current methods for detection of pathogens offer real-time on site solutions, are capable of delivering a simple visual detection signal, or can be easily instrumented as an indicator of the presence of a pathogen in water. The use of lipid vesicles incorporating Polydiacetylenes (PDAs) for the development of biosensors for “real-time” detection of pathogens has become an alternative, due to its potential for simple colorimetric response against harmful...
Tipo: Journal article Palavras-chave: Bacterial detection; Biosensors; Lipid vesicles; PDA.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100004
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