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Registros recuperados: 58 | |
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SOUZA, M. M. de; ZERLOTINI NETO, A.; GEISTLINGER, L.; TIZIOTO, P. C.; TAYLOR, J. F.; ROCHA, M. I. P.; DINIZ, W. J. S.; COUTINHO, L. L.; REGITANO, L. C. de A.. |
Transcription factors (TFs) are pivotal regulatory proteins that control gene expression in a contextdependent and tissue-specific manner. In contrast to human, where comprehensive curated TF collections exist, bovine TFs are only rudimentary recorded and characterized. In this article, we present a manually-curated compendium of 865 sequence-specific DNA-binding bovines TFs, which we analyzed for domain family distribution, evolutionary conservation, and tissue-specific expression. In addition, we provide a list of putative transcription cofactors derived from known interactions with the identified TFs. Since there is a general lack of knowledge concerning the regulation of gene expression in cattle, the curated list of TF should provide a basis for an... |
Tipo: Artigo de periódico |
Palavras-chave: DNA bovino; Expressão gênica; Gene expression in cattle; Gene expression regulation. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102174 |
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SOUZA, M. M. de; ZERLOTINI NETO, A.; GEISTLINGER, L.; TIZIOTO, P. C.; TAYLOR, J. F.; ROCHA, M. I. P.; DINIZ, W. J. S.; COUTINHO, L. L.; REGITANO, L. C. de A.. |
Transcription factors (TFs) are pivotal regulatory proteins that control gene expression in a contextdependent and tissue-specific manner. In contrast to human, where comprehensive curated TF collections exist, bovine TFs are only rudimentary recorded and characterized. In this article, we present a manually-curated compendium of 865 sequence-specific DNA-binding bovines TFs, which we analyzed for domain family distribution, evolutionary conservation, and tissue-specific expression. In addition, we provide a list of putative transcription cofactors derived from known interactions with the identified TFs. Since there is a general lack of knowledge concerning the regulation of gene expression in cattle, the curated list of TF should provide a basis for an... |
Tipo: Separatas |
Palavras-chave: DNA bovine; Gene expression in cattle; Gene expression regulation. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101203 |
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VERARDO, L. L.; STAFUZZA, N. B.; MUNARI, D. P.; ZERLOTINI NETO, A.; CHUD, T. C. S.; GARRICK, D. J.; COLE, J. B.; PANETTO, J. C. do C.; MACHADO, M. A.; MARTINS, M. F.; SILVA, M. V. G. B.. |
The aim of this study was to analyze whole-genome re-sequencing data focusing on SNVs and InDels identified in Gir, Girolando and Holstein cattle breeds related to RFI. Thus, genes showing SNVs/InDels in TF binding sites (5' UTR variants) were used to search for TF related to feed intake and to generate a gene-TF network for RFI across two purebred and one admixed dairy cattle breeds. Moreover, we were able to perform a comparative analysis accessing similarities and dissimilarities at the genomic level across these breeds. |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Sequência de dados; Single Nucleotide Variations (SNVs); Insertions/deletions (InDels); Whole-genome; Whole-genome re-sequencing data; Gado de Corte; Bos Indicus; Variação Genética; Cattle breeds. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105518 |
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SOUZA, M. M.; MOKRY, F. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A.; CESAR, A. S. M.; MORÉ, D.; MOURÃO, G.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; ZERLOTINI NETO, A.; REGITANO, L. C.. |
Imprinted genes have been target of many studies, mainly in human and mouse, and lately in bovines due to the interest of understanding the epigenetic mechanisms underlying important meat quality phenotypes and the possibility of applying it in animal breeding programs in the future. Genomic DNA from 146 steers was genotyped using the Illumina BovineHD BeadChip in order to identify heterozygous individuals with known allele origin. |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Bioinformática; Expressão gênica; Genoma; Bioinformatics; Gene expression; Genome. |
Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035220 |
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SOUZA, M. M. de; ZERLOTINI NETO, A.; ROCHA, M. I. P.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; CARDOSO, T. F.; CESAR, A. S. M.; AFONSO, J.; ANDRADE, B. G. N.; MUDADU, M. de A.; MOKRY, F. B.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; NICIURA, S. C. M.; COUTINHO, L. L.; REGITANO, L. C. de A.. |
Differences between the expression of the two alleles of a gene are known as allele-specific expression (ASE), a common event in the transcriptome of mammals. Despite ASE being a source of phenotypic variation, its occurrence and effects on genetic prediction of economically relevant traits are still unexplored in bovines. Furthermore, as ASE events are likely driven by cis-regulatory mutations, scanning them throughout the bovine genome represents a significant step to elucidate the mechanisms underlying gene expression regulation. To address this question in a Bos indicus population, we built the ASE profile of the skeletal muscle tissue of 190 Nelore steers, using RNA sequencing data and SNPs genotypes from the Illumina BovineHD BeadChip (770 K bp).... |
Tipo: Artigo de periódico |
Palavras-chave: Expressão gênica; Regulação da expressão gênica; Allelic expression; Allele-specific expression; Bos Indicus; Gene expression regulation. |
Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125395 |
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ROCHA, M. I. P.; SOUZA, M. M. de; ZERLOTINI NETO, A.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; LIMA, A. O. de; AFONSO, J.; COUTINHO, L. L.; REGITANO, L. C. de A.; NICIURA, S. C. M.. |
Tipo: Separatas |
Palavras-chave: Allele-specific expression; Genome regulation.; Genetic improvement.. |
Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1073455 |
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OLIVEIRA, P. S. N. de; COUTINHO, L. L.; TIZIOTO, P. C.; CESAR, A. S. M.; OLIVEIRA, G. B. de; DINIZ, W, J. da S.; LIMA, A. O. de; REECY, J. M.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REGITANO, L. C. de A.. |
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21,... |
Tipo: Artigo de periódico |
Palavras-chave: RFI; MiRNA; Gado Nelore; Beef cattle; Nellore; Skeletal muscle. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099755 |
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OLIVEIRA, P. S. N. de; COUTINHO, L. L.; TIZIOTO, P. C.; CESAR, A. S. M.; OLIVEIRA, G. B. de; DINIZ, W, J. da S.; LIMA, A. O. de; REECY, J. M.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REGITANO, L. C. de A.. |
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21,... |
Tipo: Artigo de periódico |
Palavras-chave: MicroRNAs; Residual Feed Intake; Gado Nelore; Beef cattle; Nellore; Skeletal muscle. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102149 |
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VIEIRA, F. D.; MOURA, D. G. de; SILVA, D. F.; HIGA, R. H.; ZERLOTINI NETO, A.. |
Nos últimos anos, o uso de genotipagem em grande escala de dezenas ou centenas de milhares de polimorfismos de nucleotídeo único (SNP) para estimar o perfil genômico de animais permitiu o desenvolvimento de estudos de associação genótipo-fenótipo em escala genômica (GWAS) e a introdução da tecnologia de seleção genômica em programas de melhoramento genético. No entanto, esta situação implica na necessidade de armazenamento de grande volume de dados de genotipagem, fenotipagem e pedigree de um elevado número de animais. Para integrar esse volume de dados distintos, é primordial utilizar uma estrutura de armazenamento robusta, como um SGBD. Assim, uma questão importante a considerar é o equilíbrio entre normalização e desempenho durante o estágio de... |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Tecnologias Java EE; Sistema Gerenciador de Banco de Dados; PostgreSQL; JavaScript Object Notation; JSON; Polimorfismo de nucleotídeo único; Banco de Dados de Genótipos e Fenótipos; Databases; Single nucleotide polymorphism; Genotype; Phenotype. |
Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1083373 |
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OLIVEIRA, P. S. N. de; COUTINHO, L. L.; CESAR, A. S. M.; DINIZ, W. J. da S.; SOUZA, M. de S.; ANDRADE, B. G.; KOLTES, J. E.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; REGITANO, L. C. de A.. |
Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were... |
Tipo: Artigo de periódico |
Palavras-chave: Ácido linoleico conjugado; Ácido oleico; Ácidos graxos; Genômica; Redes de co-expressão; Integrative genomics; MRNA; MiRNA; Bos Indicus; Gado de Corte; Beef cattle; Conjugated linoleic acid; Oleic acid. |
Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110820 |
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CHUD, T. C. S.; BICKHART, D. M.; ZERLOTINI NETO, A.; COLE, J. B.; SILVA, M. V. G. B.; MUNARI, D. P.. |
Gene copy number variants (CNV) have been shown to be associated with several production traits in dairy cattle; however, the detection and validation of CNVs in crossbred cattle is currently lacking. In order to provide a basis for future association studies, we sought to identify CNV regions (CNVRs) within the Girolando composite breed resulting from a mating of the Holstein (taurine) and Gir (indicine) breeds. A read depth method was performed using CNVnator software on NGS data from two Girolando, two Gir and ten Holstein bulls. The individual CNVs were merged into CNVRs based on genomic regions overlapping by at least 1 bp. In total, we identified a composite of 1,286 CNVRs (520 deletions, 255 duplications, 511 mixed) on the genomes of all samples. We... |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Copy number variants; Gado; Cattle; Composite breeds. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1086860 |
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HONGO, J. A.; ZERLOTINI NETO, A.; LOBO, F. P.. |
A relação ecológica de parasitismo é uma constante corrida armamentista entre os organismos parasitas e seus hospedeiros. A infecção por parasitas diminui a aptidão evolutiva dos hospedeiros e, conseqüentemente, mecanismos anti-parasitismo são positivamente selecionados continuamente dentre o conjunto de genes que compõem o genoma do organismo hospedeiro. Entretanto, a seleção positiva de mecanismos anti-parasitismo por parte dos hospedeiros impõe novas pressões seletivas aos organismos parasitas. Dessa maneira, genes de parasitas que permitam o escape dos mecanismos anti-parasitismo do hospedeiro aumentam a aptidão evolutiva do organismo parasita, sendo também selecionados positivamente. Esse fenômeno acaba por causar uma espiral de eventos coevolutivos... |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Bioinformática; Genômica; Software.; Bioinformatics; Genomics.. |
Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/933206 |
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ZERLOTINI NETO, A.; STAFUZZA, N. B.; LOBO, F. P.; YAMAGISHI, M. E. B.; CHUD, T. C. S.; CAETANO, A. R.; MUNARI, D. P.; GARRICK, D. J.; MACHADO, M. A.; MARTINS, M. F.; CARVALHO, M. R.; SILVA, M. V. G. B.. |
Guzerat is a dual-purpose breed recognized for important traits to its adaptation to adverse tropical environments such as resistance to parasites, heat tolerance and ability to intake forage with low nutritional value. Once genetic variation responsible for this traits has so far not been well characterized, the aim of this study was to identity single nucleotide variants (SNVs) and insertion/deletions (Indels) in Guzerat cattle breed from whole genome re-sequencing in order to characterize loss-of-function variants which could be associated with complex traits in this cattle breed. |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Bioinformática; Single nucleotide variants; Bioinformatics; Cattle. |
Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1067540 |
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CESAR, A. S. M.; REGITANO, L. C. de A.; POLETI, M. D.; ANDRADE, S. C. da S.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; FELICIO, A. M.; NASCIMENTO, M. L. do; CHAVES, A. S.; LANNA, D. P. D.; TULLIO, R. R.; NASSU, R. T.; KOLTES, J. E.; WATERS, E. F.; MOURAO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; COUTINHO, L. L.. |
Tipo: Artigo de periódico |
Palavras-chave: Bos Indicus; Human health; Lipids. |
Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1060542 |
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BRUSCADIN, J. J.; CARDOSO, T. F.; DINIZ, W. J. da S.; SOUZA, M. M. de; AFONSO, J.; VIEIRA, D.; MALHEIROS, J. M.; ANDRADE, B. G. N.; PETRINI, J.; FERRAZ, J. B. S.; ZERLOTINI NETO, A.; MOURÃO, G. B.; COUTINHO, L. L.; REGITANO, L. C. de A.. |
Abstract: Traditional transcriptomics approaches have been used to identify candidate genes affecting economically important livestock traits. Regulatory variants affecting these traits, however, remain under covered. Genomic regions showing allele-specific expression (ASE) are under the effect of cis-regulatory variants, being useful for improving the accuracy of genomic selection models. Taking advantage of the better of these two methods, we investigated single nucleotide polymorphisms (SNPs) in regions showing differential ASE (DASE SNPs) between contrasting groups for beef quality traits. For these analyses, we used RNA sequencing data, imputed genotypes and genomic estimated breeding values of muscle-related traits from 190 Nelore (Bos indicus)... |
Tipo: Artigo de periódico |
Palavras-chave: Polimorfismos de nucleotídeo único; Expressão gênica; Expressão alelo específica; Cis-regulation; ASE; Allele-specific expression; Fenótipo; Single nucleotide polymorphism; Gene expression; Phenotype; Beef. |
Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151168 |
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BRUSCADIN, J. J.; CARDOSO, T. F.; DINIZ, W. J. da S.; SOUZA, M. M. de; AFONSO, J.; VIEIRA, D. da S.; MALHEIROS, J.; ANDRADE, B. G.; PETRINI, J.; FERRAZ, J. B. S.; ZERLOTINI NETO, A.; MOURÃO, G.; COUTINHO, L. L. L; REGITANO, L. C. de A.. |
These findings suggest the impact of cis-regulatory variants on both the gene expression and phenotypic variation of Nelore muscle. These results can guide the investigation of regulatory variants affecting the expression of candidate genes for animal breeding, focusing on the genomic regions that affect the phenotypes of interest. |
Tipo: Resumo em anais e proceedings |
Palavras-chave: Genômica; Epigenética; Bos Indicus; Gado Nelore; Beef; Genomics; Epigenetics. |
Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1157676 |
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Registros recuperados: 58 | |
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