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Registros recuperados: 6
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Detection of SNPs in the BMP6 Gene and Their Association with Carcass and Bone Traits in Chicken 89
Cui,C; Ye,F; Li,Y; Yin,H; Ye,M; He,L; Zhao,X; Xu,H; Li,D; Qiu,M; Zhu,Q; Wang,Y.
ABSTRACT BMP6, a member of the subfamilies of the morphogenetic proteins (BMPs), plays a crucial role in osteogenic and chondrocyte differentiation in vitro and stimulates chondrogenesis, making chondrocytes differen-tiate on their terminal stage. The objective of this study is to explore the relationship between polymorphism of BMP6 gene and slaughter traits in chicken respectively. We screened the exonic and intronic regions of BMP6 gene by DNA pool construction and amplified DNA fragment by PCR, and finally, we got nine SNPs. Association analysis revealed that BMP6 had no significant association among all slaughter traits in Yellow bantam chicken. However, BMP6 had a significant difference with femur weight, tibia weight, femur length (p<0.05), and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BMP6 gene; Bone traits; Carcass traits; Chicken; Polymorphisms.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400673
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Detection of Snps in the Melanocortin 1-Receptor (MC1R) and Its Association with Shank Color Trait in Hs Chicken 89
Shen,X; Wang,Y; Cui,C; Zhao,X; Li,D; Zhu,Q; Jiang,X; Yang,C; Qiu,M; Yu,C; Li,Q; Du,H; Zhang,Z; Yin,H.
ABSTRACT The melanocortin 1 receptor (MC1R) gene plays a key role in controlling the deposition of melanin. In mammals, the MC1Rgene is regarded as a major candidate gene in the control of melanin formation. In domestic animals, the MC1R gene mainly controls the expression of coat, skin, and plumage color in mammals and birds. In order to breed chickens with dark-green shank faster, we screened the molecular markers for shank color in a HS chicken population by exploring the relationship between polymorphism of the MC1R gene and three different shank colors (light green, dark green and yellow). Two primer pairs for code region of the MC1R gene were designed in the basic of chicken genomic sequence. DNA sequencing was performed to detect the polymorphisms...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HS chicken; MC1R gene; Polymorphisms; Shank color..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300314
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Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis 89
Yang,F; He,L; Luo,B; Ye,F; Cui,C; Yu,X; Xu,H; Zhao,X; Yin,H; Li,D; Zhu,Q; Wang,Y.
ABSTRACT There is increasing evidence that bone morphogenetic protein 6 (BMP6) plays critical roles in regulating various stages of ovarian follicle development in mammals. However, the mechanisms of regulation of BMP6 in the chicken ovary remain unclear. In this study, mRNA and protein expression level of BMP6 in chicken ovarian follicles at different development stages were determined by qRT-PCR and western blot separately. Different concentrations of BMP6 protein and FSH were added to the culture medium, and the effects to proliferation of granulose cells were detected, further effect on expression pattern of progesterone synthesis associated genes were also analyzed by qRT-PCR and Western blotting and the secretion of progesterone was detected by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; BMP6; Granulosa cells; Progesterone; FSH.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200310
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Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle 89
Gao,G; Wang,H; Zhao,X; Li,Q; Wang,C; Li,J; Zhang,K; Zhong,H; Wang,Q.
ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feeding conditions; Chicken; FATP1; Meat quality; Intramuscular fat.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055
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Expression Pattern of Sulf1 and Sulf2 in Chicken Tissues and Characterization of Their Expression During Different Periods in Skeletal Muscle Satellite Cells 89
He,L; Xu,H; Ye,F; Yu,H; Lu,Y; Yin,H; Zhao,X; Zhu,Q; Wang,Y.
ABSTRACT Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. HSPGs interact with growth factors and receptors through heparan sulfate (HS) chains. The sulfation pattern of heparan sulfate chains influences signaling events mediated by heparan sulfate proteoglycans located on the cell surface. SULF1 and SULF2 are two endo-sulfatases that can cleave specific 6-O-sulfate groups within the heparan chains. To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. The SULF1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; SULF1; SULF2; Satellite cell; Immunocytochemistry.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000300307
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Molecular Authentication of Meats from Three Terrestrial Birds Based on Pcr-Rflp Analysis of the Mitochondrial 12S rRNA Gene 89
He,H; Wang,Y; Qing,Y; Li,D; Zhao,X; Zhu,Q; Yin,H.
ABSTRACT In this study, a method utilizing PCR-restriction fragment length polymorphism (PCR-RFLP) of a mitochondrial gene was developed for the identification of chicken (Gallus gallus), quail (Coturnix coturnix), and common pigeon (Columba livia) meat. PCR products of ~440 bp were obtained from the 12S rRNA gene of these three birds using a pair of universal primers. The three terrestrial birds can be distinguished using one restriction endonuclease, Alu I, which was selected based on species-specific variations in the mt 12S rRNA gene sequence using 9 newly-obtained and 44 published chicken, quail and pigeon sequences. This method was also successfully used to identify commercial quail and pigeon meat products, which were found to be adulterated with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 12S rRNA gene; PCR-RFLP; Terrestrial bird; Meat identification; Species-specific variations.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400651
Registros recuperados: 6
Primeira ... 1 ... Última
 

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