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| Yang,Yanhua; Zhu,Keming; Xia,Hengchuan; Chen,Liang; Chen,Keping. |
| Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterized. In this study, proteomic tools, including two-dimensional electrophoresis and mass spectrometry, were used to globally identify proteins that differed between two sequenced rice varieties (93-11 and Nipponbare). In all, 47 proteins that differed significantly between 93-11 and Nipponbare were identified using mass spectrometry and database searches. Interestingly, seven proteins were expressed only in Nipponbare and one protein was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Indica and japonica rice; Molecular marker; Proteomics; Quantitative real-time PCR; Unique proteins. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500008 |
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| Zhu,Keming; Tao,Huimin; Xu,Shuo; Li,Kaixia; Zafar,Sundus; Cao,Wei; Yang,Yanhua. |
| Abstract Senescence, a highly programmed process, largely determines yield and quality of crops. However, knowledge about the onset and progression of leaf senescence in crop plants is still limited. Here, we report that salt-induced protein (salT), a new gene, may be involved in leaf senescence. Overexpressing salT could prolong the duration of leaves with higher concentrations of chlorophyll compared with the wild type. Moreover, overexpression of salT could delay the senescence of rice leaves though the inhibition of senescence associated genes (SAGs). Overall, the characterization of salT suggested that it is a new gene affecting the leaf senescence induced by natural and dark conditions. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leaf senescence; Salt-induced protein (salT); Rice; Stay-green. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100080 |
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| Yang,Yanhua; Dai,Li; Xia,Hengchuan; Zhu,Keming; Liu,Haijun; Chen,Keping. |
| Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mass spectrometry; Proteomic analysis; Rice seed; Two-dimensional electrophoresis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012 |
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| Zhu,Keming; Zhang,Weiwei; Sarwa,Rehman; Xu,Shuo; Li,Kaixia; Yang,Yanhua; Li,Yulong; Wang,Zheng; Cao,Jun; Li,Yaoming; Tan,Xiaoli. |
| Abstract Rapeseed is one of important oil crops in China. Better understanding of the regulation network of main agronomic traits of rapeseed could improve the yielding of rapeseed. In this study, we obtained an influrescence mutant that showed a fusion phenotype, similar with the Arabidopsis clavata-like phenotype, so we named the mutant as Bnclavata-like (Bnclv-like). Phenotype analysis illustrated that abnormal development of the inflorescence meristem (IM) led to the fused-inflorescence phenotype. At the stage of protein abundance, major regulators in metabolic processes, ROS metabolism, and cytoskeleton formation were seen to be altered in this mutant. These results not only revealed the relationship between biological processes and inflorescence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brassica napus; Proteomic; Inflorescence meristem (IM); Bnclavata-like (Bnclv-like); Quantitative real-time PCR. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100308 |
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