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| Mirhosseini,Seyed Ziaeddin; Seidavi,Alireza; Shivazad,Mahmoud; Chamani,Mohammad; Sadeghi,Ali Asghar; Pourseify,Reza. |
| The objective of this study was to establish a specific, sensitive and rapid PCR approach for the detection of Clostridium sp. at the genus level. Clostridium sp. in the duodenum, jejunum, ileum and cecum of broiler chickens were analyzed by 16S rRNA genes. The PCR detected the presence of Clostridium spp. in naturally contaminated intestinal samples. For the total gastrointestinal segments, 53.125, 65.625 and 59.375% samples were positive for naturally occurring Clostridium spp. at the ages 4, 14 and 30d, respectively. Analysis of the microbial contents indicated that Clostridium sp. was not consistently detected in all intestinal segments. These results can put in evidence the hypothesis that Clostridium spp. may be interfering in health and performance... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium spp.; Broiler; Gastrointestinal; Age; 16S rRNA genes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100009 |
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| Rachedi,Kounouz; Zermane,Ferial; Tir,Radja; Ayache,Fatima; Duran,Robert; Lauga,Béatrice; Karama,Solange; Simon,Maryse; Boulahrouf,Abderrahmane. |
| ABSTRACT Repeated application of pesticides disturbs microbial communities and cause dysfunctions on soil biological processes. Granstar® 75 DF is one of the most used sulfonylurea herbicides on cereal crops; it contains 75% of tribenuron-methyl. Assessing the changes on soil microbiota, particularly on the most abundant bacterial groups, will be a useful approach to determine the impact of Granstar® herbicide. For this purpose, we analyzed Actinobacteria, which are known for their diversity, abundance, and aptitude to resist to xenobiotic substances. Using a selective medium for Actinobacteria, 42 strains were isolated from both untreated and Granstar® treated soils. The number of isolates recovered from the treated agricultural soil was fewer than that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Actinobacteria; Granstar® herbicide; Resistance; 16S rRNA genes. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100079 |
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| Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne. |
| Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences... |
| Tipo: Text |
Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf |
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