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A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation Biocell
Capani,Francisco; Saraceno,Ezequiel; Boti,Valeria Romina; Aon-Bertolino,Laura; Fernández,Juan Carlos; Gato,Fernando; Krause,Maria Sol; Giraldez,Lisandro; Ellisman,Mark H.; Coirini,Héctor.
Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: F-actin; Phalloidin-eosin phootoxidation; 3-D reconstructions; Electron tomography.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100001
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